FlyBase Allele Report: Dmel\Dfd[UAS.cLa]

General Information

Symbol

Dmel\Dfd^UAS.cLa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Li

FlyBase ID

FBal0094850

Feature type

allele

Associated gene

Dmel\Dfd

Associated Insertion(s)

Carried in Construct

P{UAS-Dfd.L}

Also Known As

UAS-Dfd

Key Links

Transgenic product class

wild_type

(Li et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Li et al., 1999)

Mutagen

in vitro construct

(Li et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-Dfd.L}

Cytology

Description

UASt regulatory sequences drive expression of the entire Dfd open reading frame.

(Li et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Dfd

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^arm.PS

(Lohmann et al., 2002)

lethal - all die during embryonic stage, with Scer\GAL4^arm.PS

(Li et al., 1999)

some die during embryonic stage, with Scer\GAL4^arm.PS

(Li et al., 1999)

visible, with Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005, Yao et al., 1999)

visible, with Scer\GAL4^rn.PU

(Percival-Smith et al., 2013)

Phenotype Manifest In

antennal disc, with Scer\GAL4^dpp.blk1

(Yao et al., 1999)

arista, with Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

cirri | ectopic, with Scer\GAL4^arm.PS

(Li et al., 1999)

embryonic abdominal segment, with Scer\GAL4^arm.PS

(Lohmann et al., 2002, Li et al., 1999)

embryonic head segment, with Scer\GAL4^arm.PS

(Lohmann et al., 2002)

embryonic labial segment, with Scer\GAL4^arm.PS

(Li et al., 1999)

embryonic thoracic segment, with Scer\GAL4^arm.PS

(Li et al., 1999)

eye, with Scer\GAL4^GMR.PF

(Lohmann et al., 2002)

mouth hook | ectopic, with Scer\GAL4^arm.PS

(Li et al., 1999)

pretarsus | ectopic, with Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

third segment of antenna, with Scer\GAL4^dpp.blk1

(Yao et al., 1999)

wing, with Scer\GAL4^rn.PU

(Percival-Smith et al., 2013)

Detailed Description

Statement

Reference

Expression of Dfd^Scer\UAS.cLa under the control of Scer\GAL4^rn.PU suppresses wing formation.

(Percival-Smith et al., 2013)

Expression of Dfd^Scer\UAS.cLa under the control of Scer\GAL4^dpp.blk1 results in transformation of the arista to tarsus.

(Percival-Smith et al., 2005)

When Dfd^Scer\UAS.cLa is driven by Scer\GAL4^arm.PS the number of apoptotic cells seen in the embryonic head and abdominal segments increases substantially. When Dfd^Scer\UAS.cLa is driven in the eye by Scer\GAL4^GMR.PF a mild eye ablation phenotype is seen.

(Lohmann et al., 2002)

Embryos expressing Dfd^Scer\UAS.cLa under the control of Scer\GAL4^arm.PS have ectopic mouth hook fragments and cirri. When the Scer\GAL4^arm.PS driver is provided paternally, development of these ectopic maxillary structures is restricted largely to the labial and thoracic segments. When the Scer\GAL4^arm.PS driver is provided maternally, development of these ectopic maxillary structures occurs throughout the trunk, including the abdominal segments. The number and segment-specific features of the thoracic and abdominal cuticle develop nearly normally in both cases.

(Li et al., 1999)

Strong Dfd^Scer\UAS.cLa expression in the antenna disc driven by Scer\GAL4^dpp.blk1 causes ingrowth of antennal disc tissue and produces adults with antennal third segments that are enlarged but not transformed into leg structures.

(Yao et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Dfd^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, suppressible by pb^UAS.EGFP, Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

Dfd¹⁶, Dfd^UAS.cLa has mouth hook phenotype, enhanceable by Scer\GAL4^arm.PS/cnc^B.hs

(Veraksa et al., 2000)

Dfd¹⁶, Dfd^UAS.cLa has cirri phenotype, enhanceable by Scer\GAL4^arm.PS/cnc^B.hs

(Veraksa et al., 2000)

Dfd¹⁶, Dfd^UAS.cLa, Scer\GAL4^arm.PS has mouth hook phenotype, enhanceable by cnc^B.hs

(Veraksa et al., 2000)

Suppressed by

Statement

Reference

Dfd^UAS.cLa, Scer\GAL4^dpp.blk1 has arista phenotype, suppressible by pb^UAS.EGFP, Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

Dfd^UAS.cLa, Scer\GAL4^dpp.blk1 has pretarsus | ectopic phenotype, suppressible by pb^UAS.EGFP, Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

Dfd^UAS.cLa, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Diap1^GMR.PH

(Lohmann et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of pb^{Scer\UAS.T:Avic\GFP-EGFP} suppresses the arista-to-tarsus transformation seen in flies expressing Dfd^Scer\UAS.cLa under the control of Scer\GAL4^dpp.blk1.

(Percival-Smith et al., 2005)

When th^GMR.PH is added to Dfd^Scer\UAS.cLa, Scer\GAL4^GMR.PF animals the eye phenotype is almost completely suppressed.

(Lohmann et al., 2002)

The addition of cnc^B.hs suppresses the rescuing effect seen by Dfd^Scer\UAS.cLa (when driven by Scer\GAL4^arm.PS) on Dfd¹⁶ mutants.

(Veraksa et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^arm.PS/Dfd^UAS.cLa rescues Dfd¹⁶

(Veraksa et al., 2000)

Comments

When Dfd^Scer\UAS.cLa driven by Scer\GAL4^arm.PS is added to Dfd¹⁶ mutants, the maxillary mouth hooks and cirri are restored to near-normal morphology.

(Veraksa et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

98450

w^*; P{UAS-Dfd.L}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Dfd^Scer\UAS.cLa

Dfd^UAS.cLa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Li

(Li et al., 1999)

Secondary FlyBase IDs

References (13)

Report Sections

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