FlyBase Allele Report: Dmel\Antp[UAS.cBa]

General Information

Symbol

Dmel\Antp^UAS.cBa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Bello

FlyBase ID

FBal0104893

Feature type

allele

Associated gene

Dmel\Antp

Associated Insertion(s)

Carried in Construct

P{UAS-Antp.B}

Also Known As

UAS-Antp

Key Links

Transgenic product class

cDNA

(Bello, 2000.3.15)

wild_type

(Bello, 2000.3.15)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Bello, 2000.3.15)

wild_type

(Bello, 2000.3.15)

Mutagen

in vitro construct

(Bello, 2000.3.15)

Progenitor genotype

Carried in construct

P{UAS-Antp.B}

Cytology

Description

A 2.2kb NotI fragment which contains the Antp cDNA is driven by UAS sequences.

(Bello, 2000.3.15)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Antp

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of pupal stage, with Scer\GAL4^ey-OK107

(Plaza et al., 2008)

some die during pupal stage, with Scer\GAL4^ey-OK107

(Plaza et al., 2008)

visible, with Scer\GAL4^dpp.blk1

(Plaza et al., 2008, Percival-Smith et al., 2005)

visible, with Scer\GAL4^ey-OK107

(Plaza et al., 2008)

visible, with Scer\GAL4^rn.PU

(Percival-Smith et al., 2013)

Phenotype Manifest In

antenna, with Scer\GAL4^dpp.blk1

(Plaza et al., 2008, Plaza et al., 2001)

antenna, with Scer\GAL4^ey.PH

(Prince et al., 2008)

arista, with Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

eye, with Scer\GAL4^dpp.blk1

(Plaza et al., 2008, Plaza et al., 2001)

eye, with Scer\GAL4^ey.PH

(Plaza et al., 2001)

eye, with Scer\GAL4^ey-OK107

(Plaza et al., 2008, Prince et al., 2008, Plaza et al., 2001)

eye, with Scer\GAL4^GMR.PF

(Plaza et al., 2001)

leg | ectopic, with Scer\GAL4^dpp.blk1

(Plaza et al., 2008, Plaza et al., 2001)

leg | ectopic, with Scer\GAL4^ey.PH

(Prince et al., 2008)

pretarsus | ectopic, with Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

thoracic neuroblast | larval stage, with Scer\GAL4^αTub84B.PL

(Bello et al., 2003)

wing, with Scer\GAL4^rn.PU

(Percival-Smith et al., 2013)

wing | ectopic, with Scer\GAL4^ey-OK107

(Prince et al., 2008)

Detailed Description

Statement

Reference

Expression of Antp^Scer\UAS.cBa under the control of Scer\GAL4^rn.PU suppresses wing formation.

(Percival-Smith et al., 2013)

Expression of Antp^Scer\UAS.cBa under the control of Scer\GAL4^dpp.blk1 results in antenna-to-leg transformation and a reduction in the eye.

Expression of Antp^Scer\UAS.cBa under the control of Scer\GAL4^ey-OK107 results in approximately 95% pupal lethality. 75-100% of escapers show a reduction in the eye compared to wild type.

(Plaza et al., 2008)

Misexpression of Antp^Scer\UAS.cBa under the control of Scer\GAL4^ey.PH promotes antenna-to-leg transformation but does not induce wing development.

Ectopic expression of Antp^Scer\UAS.cBa in the eye disc using the eye-specific driver Scer\GAL4^OK107 results in 8% of flies exhibiting an eye-to-wing transformation, in addition to an eye reduction phenotype.

(Prince et al., 2008)

Expression of Antp^Scer\UAS.cBa under the control of Scer\GAL4^ey.PB induces ectopic wings with antero-posterior and dorsal ventral axes.

(Katsuyama et al., 2005)

Clones in the arista expressing Antp^Scer\UAS.cBa under the control of Scer\GAL4^Act5C.PP are wild type (they are not transformed to tarsus or leg identity).

Expression of Antp^Scer\UAS.cBa under the control of Scer\GAL4^dpp.blk1 results in transformation of the arista to tarsus.

(Percival-Smith et al., 2005)

Postembryonic neuroblast (pNB) clones in the larval thorax expressing Antp^Scer\UAS.cBa under the control of Scer\GAL4^αTub84B.PL are reduced in size compared to wild-type clones and the clones show loss of the pNB in at least 82% of cases.

(Bello et al., 2003)

When Antp^Scer\UAS.cBa is driven by Scer\GAL4^ey.PH a reduction in eye size is seen. When Antp^Scer\UAS.cBa is driven by Scer\GAL4^dpp.blk1 a reduction in eye size is seen as well as antenna to leg transformations. When Antp^Scer\UAS.cBa is driven by Scer\GAL4^GMR.PF only a minor defect in eye size is seen. When Antp^Scer\UAS.cBa is driven by Scer\GAL4^OK107 an eyeless phenotype is seen.

(Plaza et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Antp^UAS.cBa, Scer\GAL4^ey-OK107 has visible phenotype, suppressible by ey^UAS.cHa, Scer\GAL4^ey-OK107

(Plaza et al., 2008)

Antp^UAS.cBa, Scer\GAL4^ey-OK107 has visible phenotype, suppressible by ey^ΔPD.UAS, Scer\GAL4^ey-OK107

(Plaza et al., 2008)

Antp^UAS.cBa, Scer\GAL4^ey-OK107 has visible phenotype, suppressible by ey^ΔCT.UAS, Scer\GAL4^ey-OK107

(Plaza et al., 2008)

Antp^UAS.cBa, Scer\GAL4^ey-OK107 has visible phenotype, suppressible by ey^ΔHD.UAS, Scer\GAL4^ey-OK107

(Plaza et al., 2008)

Antp^UAS.cBa, Scer\GAL4^dpp.blk1 has visible phenotype, suppressible by pb^UAS.EGFP, Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

NOT suppressed by

Statement

Reference

Antp^UAS.cBa, Scer\GAL4^ey-OK107 has lethal phenotype, non-suppressible by ey^ΔHD.UAS, Scer\GAL4^ey-OK107

(Plaza et al., 2008)

Other

Statement

Reference

Antp^UAS.cBa, N^ICN.UAS, Scer\GAL4^ey.PH has lethal phenotype

(Kurata et al., 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

Antp^UAS.cBa, Scer\GAL4^ey-OK107 has eye phenotype, enhanceable by Taf3^UAS.cPa/Scer\GAL4^ey.PH

(Prince et al., 2008)

Antp^UAS.cBa, Scer\GAL4^ey-OK107 has wing | ectopic phenotype, enhanceable by Taf3^UAS.cPa/Scer\GAL4^ey.PH

(Prince et al., 2008)

Antp^UAS.cBa, N^act.sev, Scer\GAL4^ey.PH has wing | ectopic phenotype, enhanceable by Taf3^UAS.cPa, Scer\GAL4^ey.PH

(Prince et al., 2008)

Antp^UAS.cBa, N^act.sev, Scer\GAL4^ey.PH has eye phenotype, enhanceable by Taf3^UAS.cPa, Scer\GAL4^ey.PH

(Prince et al., 2008)

Suppressed by

Statement

Reference

Antp^UAS.cBa, Scer\GAL4^ey-OK107 has eye phenotype, suppressible by ey^ΔCT.UAS, Scer\GAL4^ey-OK107

(Plaza et al., 2008)

Antp^UAS.cBa, Scer\GAL4^ey-OK107 has eye phenotype, suppressible by ey^UAS.cHa, Scer\GAL4^ey-OK107

(Plaza et al., 2008)

Antp^UAS.cBa, Scer\GAL4^ey-OK107 has eye phenotype, suppressible by ey^ΔPD.UAS, Scer\GAL4^ey-OK107

(Plaza et al., 2008)

Antp^UAS.cBa, Scer\GAL4^dpp.blk1 has arista phenotype, suppressible by pb^UAS.EGFP, Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

Antp^UAS.cBa, Scer\GAL4^dpp.blk1 has pretarsus | ectopic phenotype, suppressible by pb^UAS.EGFP, Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

Antp^UAS.cBa, Scer\GAL4^dpp.blk1 has eye phenotype, suppressible by ey^UAS.cHa/Scer\GAL4^ey.PH

(Plaza et al., 2001)

Antp^UAS.cBa, Scer\GAL4^dpp.blk1 has leg | ectopic phenotype, suppressible by ey^UAS.cHa, Scer\GAL4^dpp.blk1

(Plaza et al., 2001)

Antp^UAS.cBa, Scer\GAL4^dpp.blk1 has antenna phenotype, suppressible by ey^UAS.cHa, Scer\GAL4^dpp.blk1

(Plaza et al., 2001)

Suppressor of

Statement

Reference

Antp^UAS.cBa, Scer\GAL4^dpp.blk1 is a suppressor of eye | ectopic phenotype of Scer\GAL4^dpp.blk1, ey^UAS.cHa

(Plaza et al., 2001)

Other

Statement

Reference

Antp^UAS.cBa, Scer\GAL4^dpp.blk1, ey^UAS.cHa has neuron & dorsal mesothoracic disc phenotype

(Plaza et al., 2001)

Antp^UAS.cBa, N^ICN.UAS, Scer\GAL4^ey.PH has leg | ectopic phenotype

(Kurata et al., 2000)

Antp^UAS.cBa, N^ICN.UAS, Scer\GAL4^ey.PH has leg phenotype

(Kurata et al., 2000)

Antp^UAS.cBa, N^ICN.UAS, Scer\GAL4^ey.PH has head phenotype

(Kurata et al., 2000)

Antp^UAS.cBa, N^ICN.UAS, Scer\GAL4^ey.PH has wing | ectopic phenotype

(Kurata et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of ey^Scer\UAS.cHa rescues eye formation in animals expressing Antp^Scer\UAS.cBa under the control of Scer\GAL4^ey-OK107.

Co-expression of either ey^{ΔPD.Scer\UAS} or ey^{ΔCT.Scer\UAS} is able to rescue the eye loss caused by expression of Antp^Scer\UAS.cBa under the control of Scer\GAL4^ey-OK107.

Animals co-expressing ey^{ΔHD.Scer\UAS} and Antp^Scer\UAS.cBa under the control of Scer\GAL4^ey-OK107 show lethality such that few pupae are obtained. The few escapers show partial rescue of the eye loss caused by expression of Antp^Scer\UAS.cBa under the control of Scer\GAL4^ey-OK107.

(Plaza et al., 2008)

Expression of Antp^Scer\UAS.cBa and N^act.sev under the control of Scer\GAL4^ey.PH induces the ectopic outgrowth of T2-specific appendages (wings and legs) on the adult head the transformation of the anntenna into leg is not complete, owing to the fact that Scer\GAL4^ey.PH is not expressed in the entire antennal disc.

The penetrance of the eye-to-wing transformation found in Scer\GAL4^ey.PH; Antp^Scer\UAS.cBa mutants is increased upon ectopic co-expression of bip2^Scer\UAS.cPa from 8% to 24%.

Ectopic expression of bip2^Scer\UAS.cPa in combination with Antp^Scer\UAS.cBa and N^act.sev, under the control of Scer\GAL4^ey.PH strongly enhances the penetrance of eye-to-wing transformation. About 80% of these flies show eye-to-wing transformation compared with 40% without co-expression of bip2^Scer\UAS.cPa.

(Prince et al., 2008)

Co-expression of pb^{Scer\UAS.T:Avic\GFP-EGFP} suppresses the arista-to-tarsus transformation seen in flies expressing Antp^Scer\UAS.cBa under the control of Scer\GAL4^dpp.blk1.

(Percival-Smith et al., 2005)

The antenna to leg transformation phenotype in Antp^Scer\UAS.cBa, Scer\GAL4^dpp.blk1 flies is suppressed by ey^Scer\UAS.cHa. Co-expression of ey^Scer\UAS.cHa and Antp^Scer\UAS.cBa when driven by Scer\GAL4^dpp.blk1 leads to a inhibition of neuronal differentiation in the wing disc. Co-expression of ey^Scer\UAS.cHa and Antp^Scer\UAS.cBa when driven by Scer\GAL4^ey.PH represses the Antp^Scer\UAS.cBa eye phenotypes.

(Plaza et al., 2001)

Expression of Antp^Scer\UAS.cBa and N^ICN.Scer\UAS driven by Scer\GAL4^ey.PH is lethal. About 1/4 of those few escapers that do survive are found to have ectopic wings on their heads that usually consisted of dorsal and ventral wing blades bordered by bristles of the wing margin, but lacking wing veins. About 17% of these flies showed ectopic leg structures induced by secondary transformation of the ectopic antennal tissue into leg structures, an additional 10% showed a transformation of the original antenna to leg structures.

(Kurata et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Antp^Scer\UAS.cBa

Antp^UAS.cBa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Bello

(Bello, 2000.3.15, Kurata et al., 2000)

Secondary FlyBase IDs

References (11)

Report Sections

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