FlyBase Allele Report: Dmel\tws[aar-1]

General Information

Symbol

Dmel\tws^aar-1

Species

D. melanogaster

Name

FlyBase ID

FBal0032972

Feature type

allele

Associated gene

Dmel\tws

Associated Insertion(s)

P{}tws^aar-1

Carried in Construct

Also Known As

aar¹, tws^aar1

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

PM hybrid dysgenesis

(Mayer-Jaekel et al., 1993)

P-element activity

(Gomes et al., 1993)

Progenitor genotype

Associated Insertion(s)

P{}tws^aar-1

Cytology

Description

P element insertion in an intron, in the 5' intron consensus sequence.

(Mayer-Jaekel et al., 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:10,133,210..10,133,210 [+]

(Mayer-Jaekel et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

The 2.5kb, 3.7kb, and 5.0kb tws transcripts are nearly absent in brain and imaginal disc RNA from tws^aar-1 mutants. Higher molecular weight transcripts are observed which are thought to result from incorrect splicing.

(Mayer-Jaekel et al., 1993)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

chromatin & mitotic anaphase

(Williams and Goldberg, 1994)

eye

(Mayer-Jaekel et al., 1994)

macrochaeta

(Mayer-Jaekel et al., 1994)

mitotic spindle | third instar larval stage

(Mayer-Jaekel et al., 1993)

neuroblast | larval stage

(Gomes et al., 1993)

nuclear chromosome & mitotic anaphase

(Williams and Goldberg, 1994)

wing disc | larval stage

(Mayer-Jaekel et al., 1994)

Detailed Description

Statement

Reference

Homozygous or tws^j11C8/tws^aar-1 flies die as pharate adults or young adults which have duplicated bristles. tws^aar-1/Df(3R)by62 flies die as early pupae with duplicated wing imaginal discs at the third larval instar stage, or die as pharate adults with roughened eyes and duplicated bristles.

(Mayer-Jaekel et al., 1994)

Lagging chromatids and chromatin bridging are often observed during anaphase in larval brain cells.

(Williams and Goldberg, 1994)

Larval neuroblasts have a high mitotic index and chromosome abnormalities at division.

(Gomes et al., 1993)

Orcein stained brain preparations reveal mitotic abnormalities: cells, frequently hyperploid have overcondensed chromosomes, anaphases have lagging, bridged or stretched chromosomes. Whole mount preparations revealed abnormal mitotic spindles. In situ hybridizations with centromere and telomere probes showed that DNA replication was completed and sister chromatid separation occurred in lagging chromosomes, but the chromatids had not moved to the spindle poles.

(Mayer-Jaekel et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

polo[+]/polo¹¹, tws^aar-1 has female semi-sterile | dominant phenotype, suppressible | partially by endos[+]/endos^EY01105

(Rangone et al., 2011)

Enhancer of

Statement

Reference

tws^aar-1/tws[+] is an enhancer | maternal effect of female semi-sterile | dominant phenotype of gwl^scant, polo[+]/polo¹

(Rangone et al., 2011)

Suppressor of

Statement

Reference

tws^aar-1/tws[+] is a suppressor | partially of visible | adult stage phenotype of HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

tws^aar-1/tws[+] is a suppressor | partially of abnormal size phenotype of HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

tws^aar-1/tws⁶⁰ is a suppressor | partially of visible | adult stage phenotype of HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

tws^aar-1/tws⁶⁰ is a suppressor | partially of abnormal size phenotype of HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

tws^aar-1/tws[+] is a suppressor | partially of visible | adult stage phenotype of HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^en.PU

(Pechkovsky et al., 2013)

tws^aar-1/tws[+] is a suppressor | partially of abnormal cell death | third instar larval stage phenotype of HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^en.PU

(Pechkovsky et al., 2013)

Other

Statement

Reference

polo[+]/polo¹, tws^aar-1 has female fertile phenotype

(Rangone et al., 2011)

polo[+]/polo¹¹, tws^aar-1 has female semi-sterile | dominant phenotype

(Rangone et al., 2011)

gwl[+]/gwl^scant, tws^aar-1 has female semi-sterile | dominant phenotype

(Rangone et al., 2011)

gwl^scant, polo[+]/polo¹¹, tws^aar-1/tws[+] has female sterile | dominant phenotype

(Rangone et al., 2011)

gwl^scant, polo[+]/polo¹, tws^aar-1/tws[+] has female sterile | dominant phenotype

(Rangone et al., 2011)

polo¹¹, tws^aar-1/tws[+] has lethal | dominant | maternal effect | embryonic stage phenotype

(Wang et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

tws^aar-1/tws[+] is a suppressor | partially of eye phenotype of HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

tws^aar-1/tws⁶⁰ is a suppressor | partially of eye phenotype of HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

tws^aar-1/tws[+] is a suppressor | partially of wing phenotype of HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^en.PU

(Pechkovsky et al., 2013)

tws^aar-1/tws[+] is a suppressor | partially of wing disc phenotype of HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^en.PU

(Pechkovsky et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

tws^aar-1/+ enhances the reduced fertility phenotype of polo¹/+ gwl^scant/+ double heterozygous females, such that the triple heterozygotes are completely sterile.

(Rangone et al., 2011)

The embryos resulting from the cross between polo¹¹/+, tws^aar-1 mothers and wild-type fathers fail to hatch.

(Wang et al., 2011)

Xenogenetic Interactions

Statement

Reference

One copy of tws^aar-1 partially suppresses the reduction in eye size seen when Zzzz\E4orf4^Scer\UAS.cPa is expressed under the control of Scer\GAL4^GMR.PF. Eyes are 83% of wild type size in females and 74% in males.

One copy each of tws^aar-1 and tws⁶⁰ completely suppresses the reduction in eye size seen when Zzzz\E4orf4^Scer\UAS.cPa is expressed under the control of Scer\GAL4^GMR.PF.

One copy of tws^aar-1 partially suppresses the wing phenotype seen when Zzzz\E4orf4^Scer\UAS.cPa is expressed under the control of Scer\GAL4^en.PU at 29[o]C. Loss of the posterior part of the wing is seen in 8% of flies, compared to 100% when Zzzz\E4orf4^Scer\UAS.cPa is expressed alone. Many wings exhibit more minor phenotypes and in 25% of flies the wings appear wild type.

One copy of tws^aar-1 partially suppresses the wing disc phenotype seen when Zzzz\E4orf4^Scer\UAS.cPa is expressed under the control of Scer\GAL4^en.PU at 29[o]C. Only 30% of wing discs contain a cluster of dead cells, compared to 100% when Zzzz\E4orf4^Scer\UAS.cPa is expressed alone.

(Pechkovsky et al., 2013)

Complementation and Rescue Data

Rescued by

tws^aar-1 is rescued by tws^+tMa

(Mayer-Jaekel et al., 1993)

Not rescued by

tws^aar-1 is not rescued by tws^HN142

(Mayer-Jaekel et al., 1993)

Comments

Rescued by P{tws⁺=HN137}? but not P{tws^HN142}?.

(Mayer-Jaekel et al., 1993)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

aar¹

(Pechkovsky et al., 2013, Craig and Brink, 1996, Mayer-Jaekel and Hemmings, 1995, Mayer-Jaekel and Hemmings, 1994, Mayer-Jaekel et al., 1994, Gomes et al., 1993, Mayer-Jaekel et al., 1993, Mayer-Jaekel et al., 1993)

tws^aar-1

(Wang et al., 2011)

tws^aar1

(Larouche et al., 2021, Garrido et al., 2020, Rangone et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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