larval brain & neuroblast
neuroblast & larval brain
trolsd mutant larvae display morphological defects in the brain: a neural invasion phenotype (neural cells from the brain invade adjacent peripheral structures) as well as protrusions of varied size from the brain and nerve cord, however, the penetrance is relatively low as the larvae typically arrest in the second instar larval stage.
Neuroblasts in trolsd larval brains show a proliferation defect.
Strong allele. Shows a proliferation defect in the larval CNS.
Small misshapen larval brain lobes, complete lack of dividing cells in the ventral ganglion and one third of the normal number in brain lobes of third instar larvae. Failure to activate proliferation of quiescent optic lobe neuroblasts at 16-20 hours posthatching. Thoracic neuroblasts are present in larvae but are not mitotically active.
Abnormal larval brain lobe: vacuolation of cortex primarily in the region of the optic lobe proliferation centres. Gynandromorph analysis suggests that the optic lobe phenotype is independent of the genotype of the compound eye.
trolsd has decreased occurrence of cell division | larval stage phenotype, suppressible by CycBhs.PK
trolsd has decreased occurrence of cell division | larval stage phenotype, suppressible by CycEhs.PR
trolsd has decreased occurrence of cell division | larval stage phenotype, suppressible by eve[+]/eve4
trolsd has decreased occurrence of cell division | larval stage phenotype, suppressible by eve[+]/eve5
trolsd has abnormal developmental rate | larval stage phenotype, non-suppressible by AdamTS-AUAS.RB/Scer\GAL4repo
trolsd/trolsd is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of AdamTS-AUAS.RB, Scer\GAL4repo
trolsd is a non-suppressor of increased occurrence of cell division | third instar larval stage phenotype of ana8
trolsd/trolsd is a non-suppressor of larval ventral nerve cord | third instar larval stage phenotype of AdamTS-AUAS.RB, Scer\GAL4repo
trolsd is a non-suppressor of thoracic neuroblast | third instar larval stage phenotype of ana8
trolsd is a non-suppressor of adult optic lobe | third instar larval stage phenotype of ana8
trolsd is a non-suppressor of neuroblast | third instar larval stage phenotype of ana8
Expression of AdamTS-AUAS.RB under the control of Scer\GAL4repo does not suppress the larval growth defects characteristic for trolsd mutants and the larvae still arrest their development at second instar stage.
The abnormal elongation of ventral nerve cord observed in third instar larvae expressing AdamTS-AUAS.RB under the control of Scer\GAL4repo is not prevented by homozygosity of trolsd.
Expression of CycEhs.PR in trolsd larvae using heat shock is able to rescue the proliferation defect; wild-type levels of S phase neuroblasts are seen in the brain. This rescue is seen when heat shock is given at any time during the first larval instar stage and does not result in premature neuroblast proliferation. Expression of CycBhs.PK in trolsd larvae using heat shock is unable to rescue the proliferation defect; there is no change in the number of S phase neuroblasts in the brain. Expression of both Dphs.PD and E2fhs.PD in trolsd larvae using heat shock is able to rescue the proliferation defect.
Heteroallelic combinations are fully lethal.