FlyBase Allele Report: Dmel\CycB[hs.PK]

General Information

Symbol

Dmel\CycB^hs.PK

Species

D. melanogaster

Name

heat shock construct of Knoblich

FlyBase ID

FBal0029136

Feature type

allele

Associated gene

Dmel\CycB

Associated Insertion(s)

Carried in Construct

P{hs-CycB}

Also Known As

hs-cycB

Key Links

Transgenic product class

cDNA

(Knoblich and Lehner, 1993)

wild_type

(Knoblich and Lehner, 1993)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Knoblich and Lehner, 1993)

wild_type

(Knoblich and Lehner, 1993)

Mutagen

in vitro construct

(Sprenger et al., 1997, Knoblich and Lehner, 1993)

Progenitor genotype

Carried in construct

P{hs-CycB}

Cytology

Description

Expression of CycB cDNA driven from a heat shock promoter.

(Knoblich and Lehner, 1993)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

CycB

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

wild-type

(Knoblich and Lehner, 1993)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of CycB^hs.PK does not induce S phase. S phase is not induced in embryos when cdc2^AF.hs is co-expressed with CycB^hs.PK using heat shock.

(Sprenger et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

CycB^hs.PK is a suppressor | partially of abnormal mitotic cell cycle | maternal effect phenotype of png¹⁰⁵⁸/png³³¹⁸

(Lee et al., 2001)

CycB^hs.PK is a suppressor of abnormal mitotic cell cycle | maternal effect phenotype of png¹⁰⁵⁸

(Lee et al., 2001)

CycB^hs.PK is a suppressor of decreased occurrence of cell division | larval stage phenotype of trol^sd

(Caldwell and Datta, 1998)

NOT Suppressor of

Statement

Reference

Cdk1^AF.hs, CycB^hs.PK, CycB3^hs.PS is a non-suppressor of abnormal mitotic cell cycle | embryonic stage phenotype of CycA^C8LR1

(Reber et al., 2006)

Other

Statement

Reference

Cdk1^AF.hs, CycB^hs.PK has increased occurrence of cell division | embryonic stage phenotype

(Su et al., 1998)

Phenotype Manifest In

Suppressor of

Statement

Reference

CycB^hs.PK is a suppressor of mitotic cell cycle phenotype of CycA³

(Knoblich and Lehner, 1993)

NOT Suppressor of

Statement

Reference

Cdk1^AF.hs, CycB^hs.PK, CycB3^hs.PS is a non-suppressor of embryonic epidermis phenotype of CycA^C8LR1

(Reber et al., 2006)

Other

Statement

Reference

Cdk1^AF.hs, CycB^hs.PK has mitotic cell cycle phenotype

(Su et al., 2000)

Cdk1^AF.hs, CycB^hs.PK has mitotic domain 4 phenotype

(Su et al., 2000)

Cdk1^AF.hs, CycB^hs.PK has primordial germ cell phenotype

(Su et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Coexpression of cdc2^AF.hs, CycB^hs.PK and CycB3^hs.PS does not suppress this phenotype in CycA^C8LR1 mutant embryos fails to restore mitosis 16 in the epidermis.

(Reber et al., 2006)

Many cells of heatshocked embryos carrying cdc2^AF.hs and CycB^hs.PK fail to delay mitosis after irradiation.

(Su et al., 2000)

Neuroblasts in trol^sd larval brains show a proliferation defect. Expression of CycB^hs.PK in trol^sd larvae using heat shock is unable to rescue this defect; there is no change in the number of S phase neuroblasts in the brain.

(Caldwell and Datta, 1998)

Heat induced coexpression of CycB^hs.PK and Cdk1^AF.hs induces proliferation in quiescent pole cells. Ectopic mitoses are followed by cell division in pole cells.

(Su et al., 1998)

Rescues mutant phenotype of embryos double mutant for CycA³.

(Knoblich and Lehner, 1993)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

CycB^hs.PK rescues Df(2R)59AB

(Knoblich and Lehner, 1993)

Comments

Images (0)