FlyBase Allele Report: Dmel\sti[3]

General Information

Symbol

Dmel\sti³

Species

D. melanogaster

Name

FlyBase ID

FBal0032821

Feature type

allele

Associated gene

Dmel\sti

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl nitrosourea

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(D'Avino et al., 2004, Carpenter, 1994)

Mutagen

ethyl nitrosourea

(Carpenter, 1994)

Progenitor genotype

Cytology

Description

The mutation results in a truncated protein only encoding part of the kinase domain.

(D'Avino et al., 2004)

Amino acid replacement: K348term.

(D'Avino et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:12,513,243..12,513,243 [-]

Nucleotide change:

A12513243T

Amino acid change:

K348term | sti-PA; K352term | sti-PB

Reported amino acid change:

K348term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(D'Avino et al., 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle

(Carpenter, 1994)

abnormal size | larval stage (with sti¹)

(D'Avino et al., 2004)

lethal | heat sensitive (with sti^Z3-5829)

(Sweeney et al., 2008)

lethal - all die before end of larval stage (with Df(3L)F10)

(D'Avino et al., 2004)

lethal - all die before end of prepupal stage (with sti¹)

(D'Avino et al., 2004)

lethal - all die before end of prepupal stage (with sti²)

(D'Avino et al., 2004)

lethal - all die before end of prepupal stage (with sti⁴)

(D'Avino et al., 2004)

partially lethal - majority die | adult stage (with sti⁵)

(D'Avino et al., 2004)

partially lethal - majority die | pharate adult stage (with sti⁵)

(D'Avino et al., 2004)

some die during prepupal stage | recessive (with sti¹)

(D'Avino et al., 2004)

some die during prepupal stage | recessive (with sti²)

(D'Avino et al., 2004)

some die during prepupal stage | recessive (with sti⁴)

(D'Avino et al., 2004)

some die during third instar larval stage (with Df(3L)F10)

(D'Avino et al., 2004)

suppressor of variegation | dominant, with In(1)w^m4h

(Sweeney et al., 2008)

suppressor of variegation | dominant, with P{lacW}50C.x6

(Sweeney et al., 2008)

visible | heat sensitive (with sti^Z3-5829)

(Sweeney et al., 2008)

Phenotype Manifest In

eye, with Scer\GAL4^ey.PB, sti^RNAi.Sym.UAS

(D'Avino et al., 2004)

eye | heat sensitive (with sti^Z3-5829)

(Sweeney et al., 2008)

imaginal disc (with sti¹)

(D'Avino et al., 2004)

imaginal disc (with sti²)

(D'Avino et al., 2004)

imaginal disc (with sti⁴)

(D'Avino et al., 2004)

interommatidial bristle | heat sensitive (with sti^Z3-5829)

(Sweeney et al., 2008)

nucleus & follicle cell | conditional - heat sensitive (with sti^Z3-5829)

(Sweeney et al., 2008)

ommatidium, with Scer\GAL4^ey.PB, sti^RNAi.Sym.UAS

(D'Avino et al., 2004)

Detailed Description

Statement

Reference

The fraction of sti^Z3-5829/sti³ individuals recovered in a cross between sti^Z3-5829/TM6B and sti³/TM6B animals is significantly less than expected at 25[o]C, whereas at 18[o]C, there is no significant difference between the fraction of heterozygous and sti^Z3-5829/sti³ progeny recovered. At 18[o]C, both male and female sti^Z3-5829/sti³ progeny are equally represented, whereas at 25[o]C, male sti^Z3-5829/sti³ progeny are significantly reduced.

The nuclear diameter of stage 12-13 sti^Z3-5829/sti³ follicle cells is normal at 18[o], but is increased compared to wild type at 21[o]C.

In sti^Z3-5829/sti³ flies, 100% have missing eye bristles and 37% have rough eyes when reared at 21[o]C. At 25[o]C, 100% of sti^Z3-5829/sti³ adults have missing eye bristles and rough eyes.

(Sweeney et al., 2008)

The rough eye phenotype of sti^{dsRNA.Sym.Scer\UAS} expression under the regulation of Scer\GAL4^ey.PB is enhanced by the presence of sti³. sti¹/sti³, sti²/sti³, and sti⁴/sti³ flies have very small imaginal discs and are lethal at the prepupal stage.

(D'Avino et al., 2004)

In trans to a deficiency, mitotic chromosomes (both sisters and heterologues) stuck together and show metaphase delay; "successful" anaphase fragments the chromosomes.

(Carpenter, 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

sti³/sti[+] is a suppressor of visible phenotype of AGO1^UAS.cZa, Scer\GAL4^GMR.PF

(Sweeney et al., 2008)

Phenotype Manifest In

Suppressor of

Statement

Reference

sti³/sti[+] is a suppressor of eye phenotype of AGO1^UAS.cZa, Scer\GAL4^GMR.PF

(Sweeney et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

The rough and small eye phenotypes of flies expressing AGO1^Scer\UAS.cZa under the control of Scer\GAL4^GMR.PF are suppressed by sti³/+, with suppression consistently being stronger in females than in males.

(Sweeney et al., 2008)

The sti³ mutation does not modify the RacGAP50C^{dsRNA.Scer\UAS} (under the regulation of Scer\GAL4^ey.PB) rough eye phenotype.

(D'Avino et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

sti^Z3-5829

(Sweeney et al., 2008)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

4871

sti³ rn^roe-1 p^p/TM3, Sb¹ Ser¹

Kyoto

107921

sti³ rn^roe-1 p^p/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

Carpenter.

(Carpenter, 1994)

Comments

Based on lethal phase analysis the following sti alleles can be ranked from the most severe to the weakest as follows: sti³ > sti¹ = sti² = sti⁴ > sti⁵.

(D'Avino et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

sti³

(Tran et al., 2019, Bivik et al., 2015, Sweeney et al., 2008, D'Avino et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (5)

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