FlyBase Allele Report: Dmel\sti[RNAi.Sym.UAS]

General Information

Symbol

Dmel\sti^RNAi.Sym.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0190047

Feature type

allele

Associated gene

Dmel\sti

Associated Insertion(s)

Carried in Construct

P{Sym-UAS-sti-RNAi}

Key Links

Transgenic product class

RNAi_reagent

(D'Avino et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(D'Avino et al., 2004)

Mutagen

in vitro construct

(D'Avino et al., 2004)

Progenitor genotype

Carried in construct

P{Sym-UAS-sti-RNAi}

Cytology

Description

UAS regulatory sequences drive expression of sti coding sequences corresponding to most of exons 4 and 5 of sti, in a 2.5kb EcoR1 fragment.

(D'Avino et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

sti

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^ey.PB

(D'Avino et al., 2004)

eye, with Scer\GAL4^ey.PB, sti³

(D'Avino et al., 2004)

nucleus, with Scer\GAL4^ey.PB

(D'Avino et al., 2004)

ommatidium, with Scer\GAL4^ey.PB

(D'Avino et al., 2004)

ommatidium, with Scer\GAL4^ey.PB, sti³

(D'Avino et al., 2004)

Detailed Description

Statement

Reference

Expression of two copies of sti^{dsRNA.Sym.Scer\UAS} under the regulation of Scer\GAL4^ey.PB leads to the formation of multinucleate cells. Emerging adults have considerably smaller eyes compared to wild-type, composed of large and disorganised ommatidia. This rough eye phenotype is enhanced by the presence of sti³.

(D'Avino et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ey.PB, sti^RNAi.Sym.UAS has eye phenotype, enhanceable by Rho1^72O/Rho1[+]

(D'Avino et al., 2004)

Scer\GAL4^ey.PB, sti^RNAi.Sym.UAS has ommatidium phenotype, enhanceable by Rho1^72O/Rho1[+]

(D'Avino et al., 2004)

Suppressed by

Statement

Reference

Scer\GAL4^ey.PB, sti^RNAi.Sym.UAS has eye phenotype, suppressible by Rac1^J11/Rac2^Δ/Mtl[+]/Mtl^Δ/Rac2[+]/Rac1[+]

(D'Avino et al., 2004)

Scer\GAL4^ey.PB, sti^RNAi.Sym.UAS has ommatidium phenotype, suppressible by Rac1^J11/Rac2^Δ/Mtl[+]/Mtl^Δ/Rac2[+]/Rac1[+]

(D'Avino et al., 2004)

Scer\GAL4^ey.PB, sti^RNAi.Sym.UAS has eye phenotype, suppressible by sqh^E20.E21

(D'Avino et al., 2004)

Scer\GAL4^ey.PB, sti^RNAi.Sym.UAS has ommatidium phenotype, suppressible by sqh^E20.E21

(D'Avino et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

sti^RNAi.Sym.UAS

sti^{dsRNA.Sym.Scer\UAS}

sti^{dsRNA.Sym.UAS}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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