FlyBase Allele Report: Dmel\RasGAP1[mip-w+]

General Information

Symbol

Dmel\RasGAP1^mip-w+

Species

D. melanogaster

Name

FlyBase ID

FBal0029796

Feature type

allele

Associated gene

Dmel\RasGAP1

Associated Insertion(s)

P{lacW}RasGAP1^mip-w+

Carried in Construct

Also Known As

Gap1^mip

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Buckles et al., 1992)

Mutagen

P-element activity

(Buckles et al., 1992)

Progenitor genotype

Associated Insertion(s)

P{lacW}RasGAP1^mip-w+

Cytology

Description

Insertion of a P{lacW} element.

(Buckles et al., 1992)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | recessive

(Buckles et al., 1992)

lethal | maternal effect

(Chou et al., 1993)

lethal | recessive

(Dworkin and Gibson, 2006)

visible | dominant

(Dworkin and Gibson, 2006)

visible | recessive

(Buckles et al., 1992)

Phenotype Manifest In

Detailed Description

Statement

Reference

Heterozygotes show a significant effect on wing centroid size (using Fisher's method for combining probabilities). Heterozygotes show a direct effect on the shape of the wing.

(Dworkin and Gibson, 2006)

Mosaic analysis reveals that the extra R7s in each mutant ommatidium terminate together at common sites in the wild type medulla. Gap1^mip-w+, sev¹ ommatidia still develop extra R7 cells thought the normal R7 cell is missing. When axons of Gap1^mip-w+, sev¹ mosaics reach the largely unoccupied medulla, axons will invaded surrounding sites if they are under pressure for their own sites. Thus in sev¹ mosaics, an empty section in the R7 map of the medulla results, but R7 axons do not invade from the wild type, uncrowded section of the medulla.

(Ashley and Katz, 1994)

Homozygous females lay eggs with a weak dorsalized egg shell morphology. The embryos are also partially dorsalized.

(Chou et al., 1993)

The optic column contains extra axons in homozygotes. Instead of a single terminal field, multiple smaller terminals clustered in rosettes are present. These extra endings originate from separate axons and are not the result of excessive sprouting by single axons in the synaptic zone. The eyes have a rough appearance, with occasional fused ommatidia and extra bristles with random orientations. Every ommatidium contains excess small rhabdomeres which have the characteristics of R7 cells. This excess of inner photoreceptors is still seen in flies also mutant for ort¹ or sev^unspecified. Homozygous Gap1^mip-w+ flies show a preference for UV light in a colour-choice test, similar to wild-type flies. Approximately half of the ommatidia in homozygous pupal eye discs contain extra cone cells and extra primary pigment cells. Secondary and tertiary pigment cells are usually absent, although in some cases extra secondary and tertiary pigment cells are present on one side of an eye disc and absent on the other. Homozygous wings have extra vein material and often have deltas at the junctions of the veins and wing margin. The triple row appears somewhat disordered. The radius has 1 or 2 extra sensilla on the dorsal surface of its second most proximal region, and the number of sensilla in a ventral group in this region is somewhat variable.

(Buckles et al., 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

RasGAP1^mip-w+, Ack^UAS.cSb, Gap1[+], Scer\GAL4^GMR.PU is a suppressor of visible phenotype of hid^Ala5.GMR

(Schoenherr et al., 2012)

NOT Suppressor of

Statement

Reference

RasGAP1^mip-w+/Gap1[+] is a non-suppressor of visible phenotype of hid^Ala5.GMR

(Schoenherr et al., 2012)

RasGAP1^mip-w+, Gap1[+], Ack^K156A.UAS, Scer\GAL4^GMR.PU is a non-suppressor of visible phenotype of hid^Ala5.GMR

(Schoenherr et al., 2012)

Phenotype Manifest In

Enhancer of

Statement

Reference

RasGAP1^mip-w+/Gap1[+] is an enhancer of phenotype of aop^pok-15

(Yamamoto et al., 1996)

RasGAP1^mip-w+/Gap1[+] is an enhancer of photoreceptor cell R7 | ectopic phenotype of aop^pok-15

(Yamamoto et al., 1996)

Suppressor of

Statement

Reference

RasGAP1^mip-w+, Ack^UAS.cSb, Gap1[+], Scer\GAL4^GMR.PU is a suppressor of eye phenotype of hid^Ala5.GMR

(Schoenherr et al., 2012)

NOT Suppressor of

Statement

Reference

RasGAP1^mip-w+/Gap1[+] is a non-suppressor of eye phenotype of hid^Ala5.GMR

(Schoenherr et al., 2012)

RasGAP1^mip-w+, Gap1[+], Ack^K156A.UAS, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of hid^Ala5.GMR

(Schoenherr et al., 2012)

Other

Statement

Reference

Ack^UAS.cSb, RasGAP1^mip-w+/Gap1[+], Scer\GAL4^GMR.PU has pigment cell | increased number phenotype

(Schoenherr et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygosity for Gap1^mip-w+ does not suppress the small eye phenotype caused by W^Ala5.GMR.

Expression of Ack^{K156A.Scer\UAS} under the control of Scer\GAL4^GMR.PU in conjunction with or Gap1^mip-w+/+ does not suppress the small eye phenotype caused by W^Ala5.GMR.

Expression of Ack^Scer\UAS.cSb under the control of Scer\GAL4^GMR.PU suppresses the small eye phenotype caused by W^Ala5.GMR. Further suppression is seen if the flies are also heterozygous for Gap1^mip-w+/+.

Animals expressing Ack^Scer\UAS.cSb under the control of Scer\GAL4^GMR.PU in a Gap1^mip-w+/+ background show a 25% increase in pigment cell number when assessed at 42% pupa development.

(Schoenherr et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

RasGAP1^unspecified

(Dworkin and Gibson, 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6372

w¹¹¹⁸; P{lacW}RasGAP1^mip-w+/TM6C, Sb¹ Tb¹

Notes on Origin

Discoverer

Comments

Excision of the P{lacW} element reverts the eye and wing phenotypes.

(Buckles et al., 1992)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

Gap1^mip-w+

(Schoenherr et al., 2012)

Gap1^mip

(Yamamoto et al., 1996)

RasGAP1^mip-w+

gap1^mip

(Ashley and Katz, 1994)

mip(w⁺)

(Buckles et al., 1992)

mip-w⁺

(Dworkin et al., 2011)

mip-w[+]

(Dworkin and Gibson, 2006)

mip^w+

(Chou et al., 1993)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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