FlyBase Allele Report: Dmel\Ack[UAS.cSb]

General Information

Symbol

Dmel\Ack^UAS.cSb

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct b of Schoenherr

FlyBase ID

FBal0270230

Feature type

allele

Associated gene

Dmel\Ack

Associated Insertion(s)

Carried in Construct

P{UAS-Ack.Sb}

Key Links

Transgenic product class

wild_type

(Schoenherr et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Schoenherr et al., 2012)

Mutagen

in vitro construct

(Schoenherr et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-Ack.Sb}

Cytology

Description

UASt regulatory sequences drive expression of wild-type Ack.

(Schoenherr et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Ack

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^GMR.PU

(Schoenherr et al., 2012)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PU

(Schoenherr et al., 2012)

interommatidial bristle | increased number, with Scer\GAL4^GMR.PU

(Schoenherr et al., 2012)

Detailed Description

Statement

Reference

Flies expressing one copy of Ack^Scer\UAS.cSb under the control of Scer\GAL4^GMR.PU have slightly larger eyes than normal, while flies expressing two copies of Ack^Scer\UAS.cSb under the control of Scer\GAL4^GMR.PU have a highly disorganised eye.

Expression of Ack^Scer\UAS.cSb under the control of Scer\GAL4^GMR.PU results in an increase in the number of interommatidial bristles without significantly affecting the number of pigment cells when assessed at 42% pupa development.

(Schoenherr et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Ack^UAS.cSb, Scer\GAL4^GMR.PU is a suppressor of lethal | pharate adult stage phenotype of Scer\GAL4^GMR.PU, hid^GMR.PG, yki^KK109756

(Schoenherr et al., 2012)

Ack^UAS.cSb/Scer\GAL4^GMR.PU is a suppressor of increased cell death phenotype of hid^GMR.PG

(Schoenherr et al., 2012)

Ack^UAS.cSb/Scer\GAL4^GMR.PU is a suppressor of visible phenotype of rpr^GMR.PH

(Schoenherr et al., 2012)

Ack^UAS.cSb/Scer\GAL4^GMR.PU is a suppressor of visible phenotype of hid^Ala5.GMR

(Schoenherr et al., 2012)

aos[+], aos^Δ7, Ack^UAS.cSb, Scer\GAL4^GMR.PU is a suppressor of visible phenotype of hid^Ala5.GMR

(Schoenherr et al., 2012)

RasGAP1^mip-w+, Ack^UAS.cSb, Gap1[+], Scer\GAL4^GMR.PU is a suppressor of visible phenotype of hid^Ala5.GMR

(Schoenherr et al., 2012)

Ack^UAS.cSb/Scer\GAL4^GMR.PU is a suppressor of visible phenotype of hid^GMR.PG

(Schoenherr et al., 2012)

Other

Statement

Reference

Ack^UAS.cSb, Hsp83^GD1202, Scer\GAL4^GMR.PU, hid^Ala5.GMR has lethal phenotype

(Schoenherr et al., 2012)

Ack^UAS.cSb, Scer\GAL4^GMR.PU, hid^Ala5.GMR, yki^KK109756 has lethal phenotype

(Schoenherr et al., 2012)

Ack^UAS.cSb, Hsap\YAP1^{S94A.S127A.UAS}, Scer\GAL4^GMR.PU has visible phenotype

(Schoenherr et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

Ack^UAS.cSb/Scer\GAL4^GMR.PU is a suppressor of eye phenotype of hid^Ala5.GMR

(Schoenherr et al., 2012)

Ack^UAS.cSb/Scer\GAL4^GMR.PU is a suppressor of eye phenotype of rpr^GMR.PH

(Schoenherr et al., 2012)

aos[+], aos^Δ7, Ack^UAS.cSb, Scer\GAL4^GMR.PU is a suppressor of eye phenotype of hid^Ala5.GMR

(Schoenherr et al., 2012)

RasGAP1^mip-w+, Ack^UAS.cSb, Gap1[+], Scer\GAL4^GMR.PU is a suppressor of eye phenotype of hid^Ala5.GMR

(Schoenherr et al., 2012)

Ack^UAS.cSb/Scer\GAL4^GMR.PU is a suppressor of eye phenotype of hid^GMR.PG

(Schoenherr et al., 2012)

Ack^UAS.cSb/Scer\GAL4^GMR.PU is a suppressor of eye disc phenotype of hid^GMR.PG

(Schoenherr et al., 2012)

Other

Statement

Reference

Ack^UAS.cSb, Hsap\YAP1^{S94A.S127A.UAS}, Scer\GAL4^GMR.PU has eye phenotype

(Schoenherr et al., 2012)

Ack^UAS.cSb, Scer\GAL4^GMR.PU, aos[+]/aos^Δ7 has pigment cell | increased number phenotype

(Schoenherr et al., 2012)

Ack^UAS.cSb, RasGAP1^mip-w+/Gap1[+], Scer\GAL4^GMR.PU has pigment cell | increased number phenotype

(Schoenherr et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Ack^Scer\UAS.cSb under the control of Scer\GAL4^GMR.PU greatly suppresses the reduced eye size caused by W^GMR.PG.

Expression of Ack^Scer\UAS.cSb under the control of Scer\GAL4^GMR.PU suppresses the reduced eye size caused by rpr^GMR.PH.

Co-expression of Ack^Scer\UAS.cSb under the control of Scer\GAL4^GMR.PU suppresses the increased number of apoptotic cells seen posterior to the second mitotic wave and in the posterior region of the eye disc in the zone of compensatory proliferation in eye discs expressing W^GMR.PG.

Expression of Ack^Scer\UAS.cSb under the control of Scer\GAL4^GMR.PU suppresses the small eye phenotype caused by W^Ala5.GMR. Further suppression is seen if the flies are also heterozygous for either aos^Δ7/+ or Gap1^mip-w+/+.

Animals expressing Ack^Scer\UAS.cSb under the control of Scer\GAL4^GMR.PU in a aos^Δ7/+ or Gap1^mip-w+/+ background show a 25% increase in pigment cell number when assessed at 42% pupa development.

Co-expression of either Hsp26^KK106347 or Magi^GD10066 further reduces the size of the eye in a W^Ala5.GMR, Ack^Scer\UAS.cSb, Scer\GAL4^GMR.PU background.

Co-expression of drk^KK109233 in a W^Ala5.GMR, Ack^Scer\UAS.cSb, Scer\GAL4^GMR.PU background results in flies with eyes that are greatly reduced in size.

Co-expression of Ack^Scer\UAS.cSb and yki^Scer\UAS.cHa under the control of Scer\GAL4^GMR.PU results in a massively overgrown eye.Co-expression of Ack^Scer\UAS.cSb and yki^Scer\UAS.cHa under the control of Scer\GAL4^GMR.PU results in a massively overgrown eye.

(Schoenherr et al., 2012)

Xenogenetic Interactions

Statement

Reference

Co-expression of Ack^Scer\UAS.cSb and Hsap\YAP1^{S94A.S127A.Scer\UAS} under the control of Scer\GAL4^GMR.PU results in an increased eye size.

(Schoenherr et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ack^Scer\UAS.cSb

Ack^UAS.cSb

Name Synonyms

Saccharomyces cerevisiae UAS construct b of Schoenherr

Secondary FlyBase IDs

References (1)

Report Sections

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