FB2024_03 , released June 25, 2024
Allele: Dmel\plu3
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General Information
Symbol
Dmel\plu3
Species
D. melanogaster
Name
FlyBase ID
FBal0028483
Feature type
allele
Associated gene
Dmel\plu
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Axton et al., 1994)
Mutagen
PM hybrid dysgenesis
(Axton et al., 1994)
Progenitor genotype
plu1
(Axton et al., 1994)
Cytology
Description

Imprecise P-element excision leaving 20bp of the terminal inverted repeat sequence and an additional duplication of genomic sequence to give a total insertion of 48bp.

(Axton et al., 1994)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
2R:20,263,306..20,263,307 [-]
Inserted_sequence:

CATGATCAAATAACATCATGCTACAAGGATTTCAATCATGCTACAAGG

Comment:

Derivative of plu1 which leaves behind 20 bp of P sequence as well as duplicated host DNA, resulting in 48 bp of extraneous DNA between the indicated bases relative to the reference sequence.

(Axton et al., 1994)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Eggs from homozygous females have pronuclei which appear as abnormally large interphase nuclei.

      (Loppin et al., 2001)

      Homozygous females lay eggs in which the meiotic products and the pronucleus undergo extensive DNA replication resulting in a 'giant nuclei' phenotype. dhdJ5 does not suppress the premature initiation of DNA synthesis in double mutants.

      (Pellicena-Palle et al., 1997)

      Embryos undergo extensive DNA replication without nuclear division and form a fused giant polyploid nuclei from the male and female pronuclei and from the polar body nuclei.

      (Axton et al., 1994)

      Transheterozygotes with plu2 are female sterile and embryos produced have a giant polyploid nuclei.

      (Shamanski and Orr-Weaver, 1991)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Suppressor of
      Statement
      Reference

      plu3/plu3 is a non-suppressor of chromosome | maternal effect | cleavage stage phenotype of hd1/Df(3R)3-4, Scer\GAL4c323, hdUAS.cBa

      (Lesly et al., 2017)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      In 94% of eggs derived from Hira185b ; plu3 double homozygous females, the male pronucleus displays a typical Hira mutant phenotype, appearing very small in size and containing condensed chromosomes, whereas the female pronucleus and polar bodies are usually larger than control nuclei in these eggs. In the rest of the mutant eggs, there is limited and heterogeneous decondensation of the paternal chromatin.

      (Loppin et al., 2001)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      plu3 is rescued by plu+t3.8

      (Axton et al., 1994)
      Comments

      Mutant phenotype can be rescued by the plu+t3.8 construct.

      (Axton et al., 1994)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      plu3
      (Larson et al., 2022, Lesly et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (8)