FlyBase Allele Report: Dmel\srp[2]

General Information

Symbol

Dmel\srp²

Species

D. melanogaster

Name

FlyBase ID

FBal0016081

Feature type

allele

Associated gene

Dmel\srp

Associated Insertion(s)

Carried in Construct

Also Known As

srp^6G, srp^6G54

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Rehorn et al., 1996, Sam et al., 1996)

Mutagen

ethyl methanesulfonate

(Rehorn et al., 1996, Sam et al., 1996, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Nonsense mutation, translation of a truncated protein lacking the zinc finger domain.

(Rehorn et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:15,999,444..15,999,444 [+]

Nucleotide change:

C15999444T

Amino acid change:

Reported amino acid change:

Q226term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Rehorn et al., 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration | embryonic stage 15

(Renault et al., 2010)

abnormal cell migration | embryonic stage 16

(Renault et al., 2010)

decreased cell number | embryonic stage

(Rehorn et al., 1996)

decreased cell number | embryonic stage (with srp^neo45)

(Rehorn et al., 1996)

decreased occurrence of cell division | embryonic stage

(Rehorn et al., 1996)

lethal | recessive

(Rehorn et al., 1996)

Phenotype Manifest In

Detailed Description

Statement

Reference

In srp² mutant embryos, prospective anterior and posterior midgut domains transform into a portion of the ectodermal foregut and hindgut, respectively.

(Uddin et al., 2011)

In stage 15/16 srp² mutant embryos, most of the germ cells are tightly clumped in a ball inside the posterior midgut pocket; however a few germ cells move through and are found in a loose cluster on the other side of the gut.

(Renault et al., 2010)

In srp² mutants, the lymph-gland primordium is transformed to a pericardial cell-like fate.

(Mandal et al., 2004)

Crystal cell marker expression is lost in srp² homozygous embryos.

(Waltzer et al., 2003)

srp²/srp² embryos lack crystal cells and plasmatocytes.

(Waltzer et al., 2002)

The development of mutant embryos is arrested; they do not complete germband retraction and dorsal closure.

(Tingvall et al., 2001)

The development of the tracheal dorsal trunk appears to be normal.

(Franch-Marro and Casanova, 2000)

Homozygous and srp²/srp^neo45 transheterozygous embryos are devoid of any mature haemocytes. Fat body precursors are present in homozygous embryos but the cells do not proliferate and do not arrange to form the continuous sheet of cells, early events of fat body differentiation do not take place.

(Rehorn et al., 1996)

The number of precursor fat cells is reduced by stage 14/15 compared to wild-type and they are missing by late stage 15 in homozygous embryos. Homozygous embryos have an increased amount of cell death in the regions corresponding to the developing fat cells.

(Sam et al., 1996)

Mutants become distinguishable from wild type during stage 10 of embryogenesis. Cells of the prospective posterior midgut fail to lose their epithelial character and form a mesenchyme. Instead cells form a large cavity that is contiguous with the hindgut, composed of a columnar epithelium resembling the epithelium of the hindgut. No prospective anterior midgut cells, normally derived from the tip of the ventral furrow, attach to the posterior side of the stomodeum, and the anterior part of the midgut is not formed. The anterior part of the digestive tract becomes a blind-ended tube of ectodermal foregut. The endoderm fails to differentiate. The prospective anterior midgut acquires properties of the ectodermal foregut. Rudimentary Malpighian tubules develop at the correct position. In some older embryos uric acid is detectable within the lumen of the hindgut and epithelial cavity.

(Reuter, 1994)

Defective in gonad assembly.

(Warrior, 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

srp²/srp² is a suppressor of increased cell death | germline clone | maternal effect | embryonic stage phenotype of wun2^EP2650ex34, wun⁴⁹

(Renault et al., 2010)

srp²/srp² is a suppressor of decreased cell number | germline clone | maternal effect | embryonic stage 15 phenotype of wun2^EP2650ex34, wun⁴⁹

(Renault et al., 2010)

srp²/srp² is a suppressor of decreased cell number | germline clone | maternal effect | embryonic stage 16 phenotype of wun2^EP2650ex34, wun⁴⁹

(Renault et al., 2010)

NOT Suppressor of

Statement

Reference

srp[+]/srp² is a non-suppressor of decreased cell number | germline clone | maternal effect | embryonic stage 15 phenotype of wun2^EP2650ex34, wun⁴⁹

(Renault et al., 2010)

srp[+]/srp² is a non-suppressor of decreased cell number | germline clone | maternal effect | embryonic stage 16 phenotype of wun2^EP2650ex34, wun⁴⁹

(Renault et al., 2010)

srp[+]/srp² is a non-suppressor of increased cell death | germline clone | maternal effect | embryonic stage phenotype of wun2^EP2650ex34, wun⁴⁹

(Renault et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

srp² has midgut phenotype, enhanceable by Kr²

(Uddin et al., 2011)

NOT suppressed by

Statement

Reference

srp² has embryonic/larval crystal cell phenotype, non-suppressible by lz^UAS.cBa/Scer\GAL4^twi.PG

(Waltzer et al., 2003)

Enhancer of

Statement

Reference

srp² is an enhancer of Malpighian tubule phenotype of Kr²

(Uddin et al., 2011)

Suppressor of

Statement

Reference

srp²/srp² is a suppressor of germline cell | germline clone | maternal effect | embryonic stage 15 phenotype of wun2^EP2650ex34, wun⁴⁹

(Renault et al., 2010)

srp²/srp² is a suppressor of germline cell | germline clone | maternal effect | embryonic stage 16 phenotype of wun2^EP2650ex34, wun⁴⁹

(Renault et al., 2010)

NOT Suppressor of

Statement

Reference

srp[+]/srp² is a non-suppressor of germline cell | germline clone | maternal effect | embryonic stage 15 phenotype of wun2^EP2650ex34, wun⁴⁹

(Renault et al., 2010)

srp[+]/srp² is a non-suppressor of germline cell | germline clone | maternal effect | embryonic stage 16 phenotype of wun2^EP2650ex34, wun⁴⁹

(Renault et al., 2010)

Other

Statement

Reference

Kr², srp² has embryonic small intestine phenotype

(Uddin et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Kr² srp² double mutants exhibit a loss of midgut, malpighian tubules and small intestine tissue.

(Uddin et al., 2011)

Homozygous srp² rescues the germ cell death seen in embryos derived from females carrying wun⁴⁹ wun2^EP2650ex34 germline clones. The surviving germ cells are found in a tight clump inside the gut but also frequently in loosely associated groups outside of the gut. These germ cells are of normal size and morphology. Heterozygous srp² is unable to rescue the phenotype.

Homozygous srp² suppresses the germ cell death seen at embryonic stage 15/16 when wun2^{Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^twi.PG.

(Renault et al., 2010)

The loss of crystal cell marker expression in srp² homozygous embryos is not suppressed by lz^Scer\UAS.cBa; Scer\GAL4^twi.PG.

(Waltzer et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

srp² is partially rescued by Scer\GAL4^twi.PG/srp^NC.UAS

(Waltzer et al., 2002)

srp² is partially rescued by Scer\GAL4^twi.PG/srp^C.UAS

(Waltzer et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

srp²

(Uddin et al., 2011, Okumura et al., 2007)

srp^6G54

(Franch-Marro and Casanova, 2000, Sam et al., 1996)

srp^6G

(Moussalem et al., 2021, Crozatier et al., 2004, Mandal et al., 2004, Waltzer et al., 2003, Waltzer et al., 2002, Tingvall et al., 2001, Rehorn et al., 1996, Reuter, 1994, Warrior, 1994, Tearle and Nusslein-Volhard, 1987)

srp^6g

(Renault et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

Open Close