FlyBase Allele Report: Dmel\wg[spd-fg]

General Information

Symbol

Dmel\wg^spd-fg

Species

D. melanogaster

Name

flag

FlyBase ID

FBal0015993

Feature type

allele

Associated gene

Dmel\wg

Associated Insertion(s)

Carried in Construct

Also Known As

spd^flg, spd^fg

Key Links

Allele class

Mutagen

spontaneous

Nature of the Allele

Allele class

Mutagen

spontaneous

(Neumann and Cohen, 1996)

Progenitor genotype

Cytology

Description

Lesion in an enhancer element.

(Buratovich et al., 1997)

A small deletion in the 5' regulatory region of wg.

(Neumann and Cohen, 1997)

Regulatory mutation.

(Neumann and Cohen, 1996)

1kb deletion located 9kb 5' of the promoter, in the 5' regulatory region, removing an enhancer element.

(Neumann and Cohen, 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | recessive | third instar larval stage

(Dichtel-Danjoy et al., 2009)

viable

(Buratovich et al., 1997, Neumann and Cohen, 1996)

visible

(del Alamo Rodriguez et al., 2002, Neumann and Cohen, 1996)

visible | recessive

(Couso et al., 1994)

Phenotype Manifest In

Detailed Description

Statement

Reference

In wg^spd-fg mutant third instar larval wing imaginal discs, the prospective distal hinge region is reduced in size, however, there is no increase in apoptotic cells compared to wild-type.

(Dichtel-Danjoy et al., 2009)

The wing hinge region is deleted in mutant flies and the wing pouch appears directly joined to more proximal cells.

(del Alamo Rodriguez et al., 2002)

Homozygotes have shortened wings, alula etching (loss of the margin) and occasional etching of the posterior wing margin. Wings are held upward, the 'tent' phenotype. Transheterozygotes with wg alleles show an allele-dependent extent of alular etching and may show the tent phenotype. Transheterozygotes with null wg alleles only show etching of the alar lobe. Loss of Sternopleural function (by wg^Sp-1 or Df(2L)RF) exaggerates the etched wing phenotype of homozygotes.

(Buratovich et al., 1997)

wg^spd-fg/wg^spd-hL2 transheterozygotes exhibit complete loss of anterior and posterior wing margin structures and deletion of wing hinge structures.

(Neumann and Cohen, 1996)

Overall reduction in wing size, reduction of the alula and variable loss of posterior wing margin structures. wg^l-17/wg^spd-fg flies show a milder version of the hinge phenotype seen in wg^spd-fg homozygotes. A deficiency that uncovers wg does not exhibit a hinge phenotype. Heterozygotes with wg^spd-j2 produce a hinge phenotype indistinguishable from wg^spd-fg homozygotes.

(Neumann and Cohen, 1996)

Homozygotes have small wings with severely reduced alula and defects at the wing margin. When placed in trans with lethal alleles of wg, some defects in the wing margin occur and the alula is very reduced.

(Couso et al., 1994)

Wings about two-thirds the length and three-fourths the width of wild type, held tentlike over abdomen. Alulae absent or vestigial; proximal posterior wing margins often irregular with tendency to fold under about vein L4. Venation usually normal with occasional blistering. wg^spd-fg/spd has phenotype varying from slight shortening of wings to a shape midway between the two homozygotes. Excellent viability and fertility. RK1.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

wg^spd-fg has abnormal size | third instar larval stage | recessive phenotype, enhanceable by Sox15^KG09145

(Dichtel-Danjoy et al., 2009)

Suppressor of

Statement

Reference

wg^spd-fg/wg[+] is a suppressor of visible phenotype of Scer\GAL4^Bx-MS1096, hng1^KK103642

(Shukla et al., 2014)

wg^spd-fg is a suppressor | partially of hyperplasia phenotype of ft⁸/ft^G-rv

(Cho and Irvine, 2004)

Other

Statement

Reference

Sox15^KG09145, wg^spd-fg has abnormal size | recessive | third instar larval stage phenotype

(Dichtel-Danjoy et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

wg^spd-fg has wing hinge primordium phenotype, enhanceable by Sox15^KG09145

(Dichtel-Danjoy et al., 2009)

Suppressed by

Statement

Reference

wg^spd-fg/wg^spd-hL2 has wing phenotype, suppressible | partially by dsh^UAS.cNa/Scer\GAL4^vg.PM

(Neumann and Cohen, 1996)

Suppressor of

Statement

Reference

wg^spd-fg/wg[+] is a suppressor of wing hinge phenotype of Scer\GAL4^Bx-MS1096, hng1^KK103642

(Shukla et al., 2014)

wg^spd-fg is a suppressor | partially of wing disc phenotype of ft⁸/ft^G-rv

(Cho and Irvine, 2004)

Other

Statement

Reference

Sox15^KG09145, wg^spd-fg has wing hinge primordium phenotype

(Dichtel-Danjoy et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of wg^spd-fg suppresses the wing hinge phenotype seen when hng1^KK103642 is expressed under the control of Scer\GAL4^Bx-MS1096. No changes in cellular proliferation are detected in these animals.

(Shukla et al., 2014)

In wg^spd-fg, Sox15^KG09145 double mutant third instar larval wing imaginal discs, the distal hinge region is reduced in size compared to either single mutants. The rate of hinge-specific apoptosis is similar in wg^spd-fg, Sox15^KG09145 double mutant and Sox15^KG09145 single mutant L3 wing discs.

(Dichtel-Danjoy et al., 2009)

Overgrowth of the proximal wing disc in ft⁸/ft^G-rv animals is suppressed by wg^spd-fg/wg^spd-fg.

(Cho and Irvine, 2004)

Wing phenotype can be partially rescued by Scer\GAL4^vg.PM induced expression of dsh^Scer\UAS.cNa, rescue of the anterior wing margin and the posterior wing blade between vein 5 and the posterior margin.

(Neumann and Cohen, 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

wg^spd-fg/wg^spd-hL2 is partially rescued by wg^UAS.cLa/Scer\GAL4^vg.PM

(Neumann and Cohen, 1996)

Comments

Wing phenotype can be partially rescued by Scer\GAL4^vg.PM induced expression of wg^Scer\UAS.cLa rescue of the anterior wing margin and the posterior wing blade between vein 5 and the posterior margin.

(Neumann and Cohen, 1996)

Images (0)

Mutant

Wild-type

Stocks (4)

Bloomington

1005

wg^spd-fg

4963

Gpdh1^nSP4 ifc¹ wg^spd-fg pr¹/CyO, cl⁴

Kyoto

106163

wg^spd-fg

103253

cl¹ Gpdh1^B wg^spd-fg

Notes on Origin

Discoverer

Doane, 14th June 1960.

Comments

wg^spd-fg behaves as a specific regulatory allele of wg in the third instar.

(Couso et al., 1994)

Regulatory mutation of wg that specifically abolishes wg function in the hinge.

(Neumann and Cohen, 1996)

Null mutations only cause etching of the alar lobe when in combination with wg^spd-fg, but a mutation that affects the upstream sequences (Df(2L)DE) completely fails to complement wg^spd-fg demonstrating the lesion responsible is in the upstream sequences.

(Buratovich et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

Wg^spadeflag

(Shukla et al., 2014)

spd^fg

(Neumann and Cohen, 1997, Neumann and Cohen, 1996, Neumann and Cohen, 1996)

spd^flag

(del Alamo Rodriguez et al., 2002)

spd^flg

(Perea et al., 2013, Kolzer et al., 2003, Buratovich et al., 1997, Couso et al., 1994)

wg^spd-fg

(Rosales-Vega et al., 2023, Dichtel-Danjoy et al., 2009)

wg^spd

(de Celis et al., 1999)

Name Synonyms

flag

spade-flag

(Whitworth and Russell, 2000)

Secondary FlyBase IDs

References (21)

Report Sections

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