FlyBase Allele Report: Dmel\sc[10-1]

General Information

Symbol

Dmel\sc^10-1

Species

D. melanogaster

Name

FlyBase ID

FBal0015199

Feature type

allele

Associated gene

Dmel\sc

Associated Insertion(s)

Carried in Construct

Also Known As

sc^10.1

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

X ray

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Villares and Cabrera, 1987)

Mutagen

X ray

(Lindsley and Zimm, 1992)

Progenitor genotype

In(1)ac³

(Lindsley and Zimm, 1992)

Caused by aberration

Df(1)sc10-1

Cytology

Originally thought to be associated with a minute deficiency (Schultz); not confirmed by molecular analysis.

(Lindsley and Zimm, 1992)

Description

Nonsense mutation in the sc coding sequence, 162 amino acids of 345 are translated.

(Parks et al., 1997)

Point mutation that creates a stop codon within sc.

(Botella et al., 1996)

Nucleotide substitution: C1147T.

(Deshpande et al., 1995, Lindsley and Zimm, 1992, Villares and Cabrera, 1987)

Amino acid replacement: ?163term.

(Deshpande et al., 1995, Villares and Cabrera, 1987)

Amino acid replacement: Y660term.

(Lindsley and Zimm, 1992)

C669T mutation is silent. See Villares and Cabrera, 1987.

(Lindsley and Zimm, 1992)

Nucleotide substitution: C669T.

(Lindsley and Zimm, 1992, Villares and Cabrera, 1987)

Nucleotide substitution: C1143G.

(Lindsley and Zimm, 1992, Villares and Cabrera, 1987)

Amino acid replacement: S161R.

(Lindsley and Zimm, 1992, Villares and Cabrera, 1987)

Distal deficiency breakpoint is near the transcriptional start of the ac gene and the proximal breakpoint causes a nonsense mutation in the sc coding sequence.

(Rodriguez et al., 1990)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:396,663..396,663 [+]

Nucleotide change:

C396663T

Reported nucleotide change:

C1147T

Amino acid change:

Q163term | sc-PA

Reported amino acid change:

?163term

(Villares and Cabrera, 1987)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult head bristle

(Hsiao et al., 2014)

adult thoracic sensillum

(Jafar-Nejad et al., 2006)

anterior notal wing process sensillum campaniformium & glial cell

(Giangrande, 1995)

anterior notopleural bristle

(Marcellini et al., 2005)

anterior postalar bristle

(Marcellini et al., 2005)

dorsal row & glial cell

(Giangrande, 1995)

dorsal row of wing sensilla

(Jafar-Nejad et al., 2006)

eo neuron

(Jafar-Nejad et al., 2006)

macrochaeta

(Usui and Kimura, 1992, Erickson and Cline, 1991, Villares and Cabrera, 1987)

medial row of triple row of wing sensilla

(Jafar-Nejad et al., 2006)

mesothoracic leg sensillum

(Held, 1990)

mesothoracic metatarsus

(Held, 1990)

mesothoracic tarsal bristle

(Held, 1990)

microchaeta

(Usui and Kimura, 1992, Villares and Cabrera, 1987)

posterior notopleural bristle

(Marcellini et al., 2005)

posterior supraalar bristle

(Marcellini et al., 2005)

sense organ

(Rodriguez et al., 1990, Romani et al., 1989)

sensillum campaniformium of anterior crossvein & glial cell

(Giangrande, 1995)

sensillum campaniformium of dorsal radius & glial cell

(Giangrande, 1995)

sensory mother cell

(Usui and Kimura, 1992)

tormogen cell

(Jafar-Nejad et al., 2006)

ventral row & glial cell

(Giangrande, 1995)

ventral row of wing sensilla

(Jafar-Nejad et al., 2006)

wing | P-stage

(Jack et al., 1991)

wing disc

(Johnston and Edgar, 1998)

wing sensillum

(Hsiao et al., 2014)

Detailed Description

Statement

Reference

Approximately 5.5% of sc^10-1 heterozygous flies lose their post-vertical head bristles.

Removing one copy of sc in sc^10-1 heterozygous flies results in the loss of campaniform sensilla in 15% of flies.

(Hsiao et al., 2014)

The anterior wing margin of sc^10-1 pupae males is missing chemosensory bristles and shows a small but statistically significant reduction in the number of stout bristles compared to wild-type wing margins. At the pupal stage there is an absence of chemosensory clusters but there is no significant reduction of mechanosensory precursors. However, the number of neurons that develop from the mechanosensory precursors is severely reduced in sc^10-1 pupae and there is a decrease in the number of socket cells.

sc^10-1 mutants show no thoracic bristles.

(Jafar-Nejad et al., 2006)

sc^10-1 mutants show loss of the posterior supra-alar, posterior notopleural, anterior notopleural and anterior postalar thoracic bristles to varying degrees.

(Marcellini et al., 2005)

In the absence of ac and sc all cells in the ZNC of the developing wing arrest in G1 arrest.

(Johnston and Edgar, 1998)

Homozygous third instar larvae show a normal response to UVC radiation.

(Baden et al., 1996)

sc^10-1/sisA¹ female transheterozygotes have reduced viability, they are fully viable when they carry P{sc^sub}.

(Botella et al., 1996)

Glial cells of wing nerves are absent in adult wing and almost completely absent in the developing wing.

(Giangrande, 1995)

The nota of sc^10-1 flies, which are normally devoid of bristles, develop sense organs when ase^hs.PB is expressed.

(Brand et al., 1993)

Developmental capacities of germ cells homozygous for this allele were studied in pole cell transplants: fertile females and males result.

(Steinmann-Zwicky, 1993)

Like sc³ but more extreme; most extreme viable sc allele. Viability low. RK2A.

(Lindsley and Zimm, 1992)

Neither macrochaetes nor microchaetes form on the notum: no sensory mother cells develope. BrdU labelling shows that arrangement of mitotically quiescent cells is wild type.

(Usui and Kimura, 1992)

sc is expressed in a similar pattern as wild type. sc mRNA is absent from the sites where expression is originally driven by ac.

(Cubas et al., 1991)

Flies (mutant for ac³ as well as sc^10-1) show an extreme bristle phenotype.

(Erickson and Cline, 1991)

Dorsal and ventral rows of chemosensory organs are absent in pupal wings.

(Jack et al., 1991)

80% of female sc^sisB-1/sc^10-1 embryos die. All female sc^sisB-1/sc^10-1 embryos heterozygous for Sxl^f1 die. Approximately 85% of homozygous female sc^10-1 embryos die. This lethality is completely rescued by one copy of Sxl^M1. These flies survive to adulthood and show an extreme sc^10-1 phenotype. Female embryos heterozygous for sc^10-1 and deficient for one copy of sisA show 30% lethality.

(Torres and Sanchez, 1991)

The basitarsi of the second legs are reduced in width and length and have no bristles. The 8.5p sensillum is missing.

(Held, 1990)

Lack sense organs on the notum and other areas.

(Rodriguez et al., 1990)

Flies have extreme ac and sc phenotypes and are devoid of almost all sensory organs.

(Romani et al., 1989)

The adults that occasionally emerge from the pupal case lack all microchaetae and macrochaetae, except those of the eye and wing margin. The phenotype very similar to that shown by the deficiency Df(1)sc19 that lacks the sc locus.

(Villares and Cabrera, 1987)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

fl(1)35³⁵, sc^10-1 has partially lethal | female phenotype

(Anand et al., 1998)

fl(1)35⁴⁶, sc^10-1 has partially lethal | female phenotype

(Anand et al., 1998)

l(1)43⁴³, sc^10-1 has partially lethal | female phenotype

(Anand et al., 1998)

Phenotype Manifest In

Enhanced by

Statement

Reference

sc^10-1 has adult head bristle phenotype, enhanceable by Act5C^EY11969

(Hsiao et al., 2014)

sc^10-1 has adult head bristle phenotype, enhanceable by da[+]/da¹⁰

(Hsiao et al., 2014)

sc^10-1 has wing sensillum phenotype, enhanceable by Act5C^GD1351/Scer\GAL4^dpp.blk1

(Hsiao et al., 2014)

sc^10-1 has macrochaeta phenotype, enhanceable by Act5C^GD1351/Scer\GAL4^pnr-MD237

(Hsiao et al., 2014)

sc^10-1 has medial row of triple row of wing sensilla phenotype, enhanceable by sens^E2/sens[+]

(Jafar-Nejad et al., 2006)

sc^10-1 has eo neuron phenotype, enhanceable by sens^E2/sens[+]

(Jafar-Nejad et al., 2006)

sc^10-1 has tormogen cell phenotype, enhanceable by sens^E2/sens[+]

(Jafar-Nejad et al., 2006)

sc^10-1 has anterior notopleural bristle phenotype, enhanceable by ac^cami/ac[+]

(Marcellini et al., 2005)

sc^10-1 has anterior postalar bristle phenotype, enhanceable by ac^cami/ac[+]

(Marcellini et al., 2005)

sc^10-1 has posterior notopleural bristle phenotype, enhanceable by ac^cami/ac[+]

(Marcellini et al., 2005)

sc^10-1 has posterior supraalar bristle phenotype, enhanceable by ac^cami/ac[+]

(Marcellini et al., 2005)

NOT Enhanced by

Statement

Reference

sc^10-1 has adult head bristle phenotype, non-enhanceable by Act5C^G0010/Act5C[+]

(Hsiao et al., 2014)

Suppressed by

Statement

Reference

sc^10-1 has medial row of triple row of wing sensilla phenotype, suppressible by sens^+t11

(Jafar-Nejad et al., 2006)

NOT suppressed by

Statement

Reference

sc^10-1 has phenotype, non-suppressible by su(Hw)²

(Lindsley and Grell, 1968)

Enhancer of

Statement

Reference

sc[+]/sc^10-1 is an enhancer of adult head bristle phenotype of da¹⁰

(Hsiao et al., 2014)

Suppressor of

Statement

Reference

sc^10-1 is a suppressor | partially of macrochaeta | ectopic & antenna phenotype of amos¹/Df(2L)M36F-S6

(zur Lage et al., 2003)

Other

Statement

Reference

Scer\GAL4^Eq, da^UAS.cGa, sc^10-1, sens^UAS.cNa has adult thoracic sensillum phenotype

(Jafar-Nejad et al., 2006)

Scer\GAL4^Eq, da^UAS.cGa, sc^10-1 has adult thoracic sensillum phenotype

(Jafar-Nejad et al., 2006)

Scer\GAL4^Eq, sc^10-1, sens^UAS.cNa has adult thoracic sensillum phenotype

(Jafar-Nejad et al., 2006)

BacA\p35^UAS.cHa, Scer\GAL4^bbg-C96, sc^10-1 has eo neuron phenotype

(Jafar-Nejad et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Approximately 95% of sc^10-1/+; da¹⁰/+ trans-heterozygous flies exhibit missing post-vertical head bristles.

Knockdown of Act5C through expression of Act5C^GD1351 under the control of Scer\GAL4^dpp.blk1 results in a dramatic increase in the percentage of sc^10-1 flies missing campaniform sensilla. Likewise, knockdown of Act5C^GD1351 by Scer\GAL4^pnr-MD237 in the sc^10-1/+ fly notum markedly increased the percentage of flies missing anterior dorso-central mechano-sensory macrochaetae from 30% to 87%, and missing posterior dorso-central macrochaetae from 0% to 24%.

sc^10-1/+; Act5C^EY11969/+ double heterozygotes exhibit some loss of post-vertical head bristles in 45% of flies.

The percentage of the double heterozygous sc^10-1/+; +/+; Act5C^G0010/+ flies missing post-vertical head bristles are no significantly different or only mildly increased compared with sc^10-1/+ flies.

(Hsiao et al., 2014)

sc^10-1; sens^E2/+ flies show a severe loss of stout bristles at the wing margin in comparison to sc^10-1 single mutants, which show a small loss of these bristles. At the pupal level the sc^10-1; sens^E2/+ double mutants are almost completely lacking the neurons that should have developed from the mechanosensory precursors; this is an enhancement of the reduced number of neurons seen in sc^10-1 single mutant pupae. The double mutants also show a greater loss of socket cells than the single mutants.

Expressing one copy of sens^+t11 in a sc^10-1 background restores the number of stout bristles to near wild-type numbers.

Expression of da^Scer\UAS.cGa in the thorax, under the control of Scer\GAL4^Eq1, leads to the generation of numerous bristles on the thorax of sc^10-1 mutants. These mutants have no thoracic bristles without the transgene expression.

Expression of sens^Scer\UAS.cNa in the thorax, under the control of Scer\GAL4^Eq1, leads to the generation of bristles on the thorax of sc^10-1 mutants. The amount of bristles generated by sens^Scer\UAS.cNa is insertion-dependent; the weak P{UAS-sens.N}C6 insertion leads to the presence of only a few bristles, while the strong P{UAS-sens.N}C5 insertion generates more bristles.

Coexpression of da^Scer\UAS.cGa and sens^Scer\UAS.cNa in the thorax, under the control of Scer\GAL4^Eq1, leads to the generation of multiple bristles on the thorax of sc^10-1 mutants; these bristles are present at a much greater density than those on wild-type thoraces or indeed the density produced by expressing either da^Scer\UAS.cGa or sens^Scer\UAS.cNa alone.

(Jafar-Nejad et al., 2006)

sc^10-1/+, ac^cami/+ mutants experience a greater loss of the posterior supra-alar, posterior notopleural, anterior notopleural and anterior postalar bristles than sc^10-1 single mutants.

(Marcellini et al., 2005)

Does not interact with Sxl^dlf. Females doubly heterozygous for sc^10-1 and either fl(1)35³⁵ or fl(1)35⁴⁶ show reduced viability compared to single heterozygous females. Females doubly heterozygous for l(1)43⁴³ and sc^10-1 show a slight reduction in viability compared to single heterozygous females.

(Anand et al., 1998)

Xenogenetic Interactions

Statement

Reference

Expressing BacA\p35^Scer\UAS.cHa in the wing margin, under the control of Scer\GAL4^C96, in a sc^10-1 background rescues the stout bristle loss seen in sc^10-1 mutants. At the pupal level, BacA\p35^Scer\UAS.cHa, expression also rescues the loss of neurons seen in the single mutants. However, this expression also results in the appearance of ectopic neurons at the posterior wing margin, a place where such neurons are not found in wild type. These neurons send out axons which merge with the marginal nerve that runs along the anterior wing margin towards the thorax.

(Jafar-Nejad et al., 2006)

Complementation and Rescue Data

Rescued by

sc^sisB-1/sc^10-1 is rescued by sc^hs.PR

(Torres and Sanchez, 1991)

Partially rescued by

sc^10-1 is partially rescued by sc^+mDC

(Culi and Modolell, 1998)

sc^10-1 is partially rescued by sc^+mDC.SMC

(Culi and Modolell, 1998)

Comments

In female D.simulans/D.melanogaster hybrids that carry In(1)ac³ and sc^10-1, the bristle phenotype at 18^oC is no different to wild-type D.simulans/D.melanogaster hybrids. At 25^oC a bristle phenotype is seen in simulans/D.melanogaster hybrids that carry In(1)ac³ and sc^10-1 that is not as strong as wild-type D.simulans/D.melanogaster hybrids.

(Skaer and Simpson, 2000)

Bristle phenotype not rescued by sc⁺ transgenes.

(Erickson and Cline, 1991)

Expression of sc^hs.PR during the first two hours of development rescues female sc^sisB-1/sc^10-1 embryos.

(Torres and Sanchez, 1991)

Images (0)

Mutant

Wild-type

Stocks (4)

Bloomington

109

Df(1)sc10-1, sc^10-1/y¹ ac^Hw-1

6016

Df(1)sc10-1, sc^10-1 f^36a/FM6/Dp(1;Y)y²67g19.1

36541

In(1)ac³, sc^10-1 ac³ w¹ Ype^s-1/FM7i, P{ActGFP}JMR3

Kyoto

105739

Df(1)sc10-1, sc^10-1/y¹ ac^Hw-1

Notes on Origin

Discoverer

Sturtevant, 1930.

(Lindsley and Zimm, 1992)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

sc^10-1

(Couturier et al., 2019, Hsiao et al., 2014, Pi et al., 2011, Zhai et al., 2009, Jafar-Nejad et al., 2006, Joshi et al., 2006, Reeves and Posakony, 2005)

sc^10.1

(Johnston and Edgar, 1998, Gomez-Skarmeta et al., 1995, Cubas et al., 1991, Villares and Cabrera, 1987)

scute^10.1

(Garcia-Alonso, 1999)

Name Synonyms

Secondary FlyBase IDs

References (49)

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