FlyBase Allele Report: Dmel\br[rbp-2]

General Information

Symbol

Dmel\br^rbp-2

Species

D. melanogaster

Name

FlyBase ID

FBal0014442

Feature type

allele

Associated gene

Dmel\br

Associated Insertion(s)

Carried in Construct

Also Known As

rbp², rbp^t132

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Restifo and Merrill, 1994, Demakova et al., 1988, Belyaeva et al., 1982)

Mutagen

ethyl methanesulfonate

(Restifo and Merrill, 1994, Belyaeva et al., 1982)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile (with br^npr-6)

(Tzolovsky et al., 1999)

fertile

(Sandstrom et al., 1997)

flightless | partially

(Sandstrom et al., 1997)

lethal (with br^npr-5)

(Belyaeva et al., 1980)

lethal (with br^npr-6)

(Belyaeva et al., 1980)

partially lethal | recessive

(Tzolovsky et al., 1999)

some die during P-stage (with br^npr-3)

(Wilson et al., 2006)

viable

(Wilson et al., 2006, Sandstrom et al., 1997, Emery et al., 1994, Restifo and Merrill, 1994)

viable (with Df(1)br²⁵)

(Belyaeva et al., 1980)

Phenotype Manifest In

abdomen

(Belyaeva et al., 1982)

coxal tergal remotor muscle cell

(Sandstrom et al., 1997)

dorsal appendage

(Tzolovsky et al., 1999)

dorsal appendage (with br^npr-6)

(Tzolovsky et al., 1999)

eye

(Restifo and Merrill, 1994)

lateral oblique dorsal muscle cell

(Sandstrom et al., 1997)

maxillary palp bristle

(Restifo and Merrill, 1994, Belyaeva et al., 1980)

maxillary palp bristle (with br^npr-5)

(Belyaeva et al., 1980)

maxillary palp bristle (with br^npr-6)

(Belyaeva et al., 1980)

maxillary palp bristle (with Df(1)br²⁵)

(Belyaeva et al., 1980)

maxillary palpus & macrochaeta

(Belyaeva et al., 1982)

ovary

(Wilson et al., 2006)

ovary (with br^npr-6)

(Tzolovsky et al., 1999)

scutellar bristle

tergosternal muscle cell

(Sandstrom et al., 1997)

wing

(Belyaeva et al., 1982)

Detailed Description

Statement

Reference

100% of br^rbp-2/br^npr-3 larvae are able to pupate, but ~15% die as early pupae. 80% of these survive to late pharate adult stage, with ~20% failing to eclose. br^rbp-2 females show slightly reduced levels of oviposition compared to wild-type females. br^rbp-2 flies show the same susceptibility to methoprene as wild-type flies.

(Wilson et al., 2006)

Eggs dissected from homozygous br^rbp-2 mothers have shortened dorsal appendages. br^rbp-2/br^npr-6 females live for two days before dying having laid no eggs. Their ovaries are not completely developed, and the few late stage oocytes that were formed had no appendages.

(Tzolovsky et al., 1999)

DVM number is reduced (a deficit in thoracic muscle precursors), as is the flight performance.

(Sandstrom et al., 1997)

Heterozygotes with br^rbp-5 display reduced mechanosensory bristles on the maxillary palps and indentations on the surface of the eye.

(Restifo and Merrill, 1994)

Morphological abnormalities: 15--100% penetrance of bristle abnormalities under T(1;2)dor^var7 variegation.

(Demakova et al., 1988)

Reduced bristle number on palpus, faded wings and swollen abdomen. Rare survivors when heterozygous with Df(1)RA19, lethal phase is after the formation of imaginal organs.

(Belyaeva et al., 1982)

Homozygotes exhibit reduction of the palpal bristles. br^rbp-2/br^npr-5 and br^rbp-2/br^npr-6 combination is lethal, rare adult escapers show a reduction of the bristle number on the palpus. Heterozygotes with Df(1)br²⁵ have normal viability and reduced bristle number on the palpus.

(Belyaeva et al., 1980)

Homozygotes and males survive with fewer than normal bristles on the palpus; when raised at 29^oC, females exhibit faded wings and swollen abdomens; when raised at 18^oC males have faded wings. br^rbp-2/Df(1)RA19 females virtually lethal; females carrying br^rbp-2 and any of the other 'rbp' alleles are completely viable when reared at 18^oC, but at higher temperatures most die and escapers have reduced bristles on the palpus, shortened bristles on the scutellum, shrivelled or swollen abdomen shrivelled wings and eyes with crumpled surface.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

br^rbp-2/br^npr-3 has lethal - all die during P-stage phenotype, enhanceable by Met³/Met³

(Wilson et al., 2006)

Enhancer of

Statement

Reference

br^rbp-2 is an enhancer of visible phenotype of Met^W3

(Wilson et al., 2006)

Other

Statement

Reference

Met²⁷, br^rbp-2/br⁵ has lethal phenotype

(Wilson et al., 2006)

Met²⁷, br^rbp-2/br^2Bc-1 has viable phenotype

(Wilson et al., 2006)

Met²⁷, br^rbp-2 has lethal - all die during P-stage phenotype

(Wilson et al., 2006)

Met²⁷, br^rbp-2/br^rbp-1 has lethal - all die during P-stage phenotype

(Wilson et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

br^rbp-2 has ovary phenotype, enhanceable by Met²⁷/Met²⁷

(Wilson et al., 2006)

Enhancer of

Statement

Reference

br^rbp-2/br^rbp-2 is an enhancer of ovary phenotype of Met²⁷

(Wilson et al., 2006)

br^rbp-2 is an enhancer of eye phenotype of Met^W3

(Wilson et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The lethality is enhanced in br^rbp-2/br^npr-3 Rst(1)JH³/Rst(1)JH³ double mutants compared to br^rbp-2/br^npr-3 mutants, with no flies surviving beyond the pupal stage. While 86% of br^rbp-2/br^rbp-2, Rst(1)JH²⁷/+ flies survive to adulthood, <2% of br^rbp-2/br^rbp-2, Rst(1)JH²⁷/Rst(1)JH²⁷ flies survive this far, with most dying during the pharate adult stage. br^rbp-2/br^rbp-1, Rst(1)JH²⁷/Rst(1)JH²⁷ flies are also prepupal/early pupal lethals. Expression of the Rst(1)JH^+tSt-H.hs transgene can rescue the lethality of br^rbp-2/br^rbp-2, Rst(1)JH²⁷/Rst(1)JH²⁷ or br^rbp-2/br^rbp-1, Rst(1)JH²⁷/Rst(1)JH²⁷ flies. br^rbp-2/br^rbp-2, Rst(1)JH²⁷/Rst(1)JH²⁷ female escapers show strong reductions in oogenesis.

(Wilson et al., 2006)

The expressivity of the defective eye phenotype seen in Met^W3 and Met²⁷ flies is increased by br^rbp-2.

(Wilson et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

br^npr-6

(Tzolovsky et al., 1999)

Comments

Complementation is based on lethality.

(Tzolovsky et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

br^rbp-2

rbp²

(Wilson et al., 2006, Wilson et al., 2006, Tzolovsky et al., 1999, Sandstrom et al., 1997, Belyaeva et al., 1982)

rbp^t132

(Mazina et al., 1991, Mazina et al., 1990, Solovyeva and Belyaeva, 1989, Demakova et al., 1988, Belyaeva et al., 1980)

t132

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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