FlyBase Allele Report: Dmel\pho[1]

General Information

Symbol

Dmel\pho¹

Species

D. melanogaster

Name

FlyBase ID

FBal0011501

Feature type

allele

Associated gene

Dmel\pho

Associated Insertion(s)

Doc{}pho¹

Carried in Construct

Also Known As

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Brown et al., 1998)

Mutagen

natural population

(Hochman et al., 1964)

spontaneous

(Hochman, 1976, Hochman, 1974, Hochman, 1971)

Progenitor genotype

Associated Insertion(s)

Doc{}pho¹

Cytology

Description

4.5kb Doc insertion into the coding region at codon 272, upstream of the zinc finger region of the protein.

(Brown et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

4:1,174,346..1,174,348 [-]

(Brown et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

antenna (with pho^cv)

(Atchison et al., 2003)

sex comb (with pho^cv)

(Atchison et al., 2003)

unguis (with pho^cv)

(Atchison et al., 2003)

Detailed Description

Statement

Reference

Heterozygotes do not have extra sex combs on the 2nd or 3rd leg and do not show transformation of antenna to leg.

(Busturia et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

pho¹ has lethal | pharate adult stage | recessive phenotype, enhanceable by Spps¹/Spps¹

(Brown and Kassis, 2010)

Enhancer of

Statement

Reference

pho[+]/pho¹ is an enhancer of visible phenotype of E2f1^UAS.cNa, Scer\GAL4^Act88F.PD

(Ji et al., 2012)

pho¹ is an enhancer of visible phenotype of Trl^R85, Ubx¹³⁰

(Bejarano and Busturia, 2004)

NOT Enhancer of

Statement

Reference

pho[+]/pho¹ is a non-enhancer of visible | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

pho[+]/pho¹ is a non-enhancer of visible | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

pho[+]/pho¹ is a non-enhancer of visible phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.PU

(Ji et al., 2012)

Suppressor of

Statement

Reference

pho[+]/pho¹ is a suppressor of visible phenotype of E2f1^RNAi.UAS.cJa, Scer\GAL4^GMR.PU

(Ji et al., 2012)

pho[+]/pho¹ is a suppressor of visible phenotype of E2f1^RNAi.UAS.cJa, Scer\GAL4^ptc-559.1

(Ji et al., 2012)

NOT Suppressor of

Statement

Reference

pho[+]/pho¹ is a non-suppressor of visible | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

pho[+]/pho¹ is a non-suppressor of visible | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

pho[+]/pho¹ is a non-suppressor of visible phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.PU

(Ji et al., 2012)

Other

Statement

Reference

Spps¹, pho¹ has abnormal body size | larval stage phenotype

(Brown and Kassis, 2010)

pho¹, phol^81A has lethal | larval stage phenotype

(Brown et al., 2003)

Trl^R85, pho¹ has visible | homeotic phenotype

(Busturia et al., 2001)

Trl^R85, pho[+]/pho¹ has visible | homeotic phenotype

(Busturia et al., 2001)

Phenotype Manifest In

Enhanced by

Statement

Reference

pho¹ has phenotype, enhanceable by E(Pc)¹

(Lindsley and Zimm, 1992)

Suppressed by

Statement

Reference

pho^cv/pho¹ has unguis phenotype, suppressible | partially by Hsap\YY1^G4DBD.Hsp70

(Atchison et al., 2003)

pho^cv/pho¹ has antenna phenotype, suppressible by Hsap\YY1^G4DBD.Hsp70

(Atchison et al., 2003)

pho^cv/pho¹ has sex comb phenotype, suppressible by Hsap\YY1^G4DBD.Hsp70

(Atchison et al., 2003)

NOT suppressed by

Statement

Reference

pho^cv/pho¹ has antenna phenotype, non-suppressible by Hsap\YY1^arm.PA

(Atchison et al., 2003)

pho^cv/pho¹ has sex comb phenotype, non-suppressible by Hsap\YY1^arm.PA

(Atchison et al., 2003)

pho^cv/pho¹ has unguis phenotype, non-suppressible by Hsap\YY1^arm.PA

(Atchison et al., 2003)

Enhancer of

Statement

Reference

pho[+]/pho¹ is an enhancer of wing phenotype of E2f1^UAS.cNa, Scer\GAL4^Act88F.PD

(Ji et al., 2012)

NOT Enhancer of

Statement

Reference

pho[+]/pho¹ is a non-enhancer of wing vein | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

pho[+]/pho¹ is a non-enhancer of wing vein | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

pho[+]/pho¹ is a non-enhancer of eye phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.PU

(Ji et al., 2012)

Suppressor of

Statement

Reference

pho[+]/pho¹ is a suppressor of eye phenotype of E2f1^RNAi.UAS.cJa, Scer\GAL4^GMR.PU

(Ji et al., 2012)

pho[+]/pho¹ is a suppressor of 1st posterior cell phenotype of E2f1^RNAi.UAS.cJa, Scer\GAL4^ptc-559.1

(Ji et al., 2012)

pho[+]/pho¹ is a suppressor of adult abdominal segment 4 | ectopic phenotype of E(z)^Trm

(Bajusz et al., 2001)

pho[+]/pho¹ is a suppressor of adult abdominal segment 5 phenotype of E(z)^Trm

(Bajusz et al., 2001)

pho[+]/pho¹ is a suppressor of adult mesothoracic segment | ectopic phenotype of E(z)^Trm

(Bajusz et al., 2001)

pho[+]/pho¹ is a suppressor of adult metathoracic segment phenotype of E(z)^Trm

(Bajusz et al., 2001)

pho[+]/pho¹ is a suppressor of adult prothoracic segment phenotype of E(z)^Trm

(Bajusz et al., 2001)

NOT Suppressor of

Statement

Reference

pho[+]/pho¹ is a non-suppressor of wing vein | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

pho[+]/pho¹ is a non-suppressor of wing vein | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

pho[+]/pho¹ is a non-suppressor of eye phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.PU

(Ji et al., 2012)

Other

Statement

Reference

Spps¹, pho¹ has imaginal disc phenotype

(Brown and Kassis, 2010)

pho¹, phol^81A has imaginal disc phenotype

(Brown et al., 2003)

pho¹, phol^81A has embryonic/larval brain phenotype

(Brown et al., 2003)

pho¹, phol^81A has polytene chromosome phenotype

(Brown et al., 2003)

Trl^R85, pho[+]/pho¹ has sex comb | ectopic phenotype

(Busturia et al., 2001)

Trl^R85, pho¹ has mesothoracic leg phenotype

(Busturia et al., 2001)

Trl^R85, pho¹ has metathoracic leg phenotype

(Busturia et al., 2001)

Trl^R85, pho[+]/pho¹ has mesothoracic leg phenotype

(Busturia et al., 2001)

Trl^R85, pho[+]/pho¹ has metathoracic leg phenotype

(Busturia et al., 2001)

Trl^R85, pho¹ has sex comb | ectopic phenotype

(Busturia et al., 2001)

E(Pc)¹, pho¹ has embryonic head phenotype

(Lindsley and Zimm, 1992)

Additional Comments

Genetic Interactions

Statement

Reference

The formation of ectopic wing veins observed in adult flies expressing Marcal1^Scer\UAS.cBa under the control of Scer\GAL4^tub.PU is not affected by combination with a single copy of pho¹.

(Morimoto et al., 2016)

pho¹, Spps¹ double mutants die earlier in development than either single mutant: single mutants die as pharates, whereas double mutants die as third instar larvae or often at earlier stages. Double mutant larvae are often abnormally small and have misshapen imaginal discs.

(Brown and Kassis, 2010)

The eye colour of flies homozygous for w^{GMR.eve.PRE300} is slightly darker in a pho¹/+ background than in a wild-type background.

The eye colour of flies homozygous for w^{eve.RR.PRE300} is considerably darker in a pho¹/+ background than in a wild-type background.

(Fujioka et al., 2008)

The reduced wing phenotype of RYBP^Scer\UAS.cBa; Scer\GAL4^sd-SG29.1 flies is enhanced by pho¹/+.

(Bejarano et al., 2005)

Trl^R85/Ubx¹³⁰; pho¹ flies show an enhancement of the Ubx¹³⁰ phenotype in 18% of flies.

(Bejarano and Busturia, 2004)

phol^81A ; pho¹ double mutants die as third instar larvae and fail to pupate. The brain is smaller than normal, the disks are misshapen and smaller than normal and the salivary gland polytene chromosomes are enlarged in double mutant third instar larvae.

(Brown et al., 2003)

11% of Trl^R85/+ ; pho¹/+ double heterozygotes have extra sex combs (on the 2nd or 3rd leg) but they do not show transformation of antenna to leg.

(Busturia et al., 2001)

pho¹/pho^cv suppresses the pairing-dependent silencing of w^iab-7PRE.860 seen in flies homozygous for P{iab-7PRE-w.860}P24.

(Mishra et al., 2001)

Mutant heterozygotes show a moderate suppression of the eye phenotype see in P{UAS-lacZ.Abd-B.5F24} leading to darker eyes.

(Cavalli, 1999)

The addition of pho¹/pho^cv strongly suppresses the Pairing sensitive silencing effect seen in w^Mcp.10, w^Mcp.13,w^{Mcp.scs'.12:15} and w^Mcp.scs'.11. The addition of pho¹/pho^cv has no effect on the Pairing sensitive silencing effect seen in w^Mcp.11, and w^Mcp.scs'.14. The addition of pho¹/pho^cv very strongly suppresses the Pairing sensitive silencing effect seen in w^Mcp.scs'.11 (P{Mcp-w#11}16/+, P{Mcp-w#11}102/+) double heterozygous insertions. The addition pho¹/pho^cv very strongly suppresses the Pairing sensitive silencing effect seen in the w^Mcp.scs'.14/ w^Mcp.13 (P{Mcp-w#14}AP-2^stummelbein/+, P{Mcp-w#13}116/+) double heterozygous insertion.

(Muller et al., 1999)

Has little or no effect on the phenotype of E2f^GMR.PD Dp^GMR.PD BacA\p35^GMR.PH flies.

(Staehling-Hampton et al., 1999)

Eye colour encoded by w^en.8 is darker in pho¹/pho¹ or pho¹/pho^cv background.

(Brown et al., 1998)

Xenogenetic Interactions

Statement

Reference

The formation of ectopic wing veins observed in adult flies expressing Hsap\SMARCAL1^Scer\UAS.cBa under the control of Scer\GAL4^Bx-MS1096 is not affected by combination with a single copy of pho¹.

(Morimoto et al., 2016)

When heatshocked twice daily, Hsap\YY1^{Hsp70.T:Scer\GAL4} nearly completely corrects the antennal and sex comb defects in pho¹/pho^cv mutant flies. Segmentation is largely normal, although pigmentation on tergite 5 is not always complete. Correction of claw structures is variable ranging from complete to partial correction.

(Atchison et al., 2003)

Complementation and Rescue Data

Rescued by

pho¹ is rescued by pho^{αTub84B.EGFP}

(Steffen et al., 2013)

pho¹ is rescued by pho^{αTub84B.Tag:TAP}

(Klymenko et al., 2006)

pho¹ is rescued by pho^{lexA.αTub84B}

(Poux et al., 2001)

Partially rescued by

pho¹ is partially rescued by pho^UAS.Tag:FLAG/Scer\GAL4^arm.PS

(Langlais et al., 2012)

pho¹ is partially rescued by pho^lexA.hs

(Poux et al., 2001)

Not rescued by

pho¹ is not rescued by pho^EGFP

(Steffen et al., 2013)

Comments

Expression of pho^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^arm.PS partially rescues pho¹ homozygotes: approximately 10% of rescued adult males show moderate A4-A5 transformations.

(Langlais et al., 2012)

Two copies of pho^{hs.T:Ecol\lexA} produces healthy pho¹/pho¹ adults with one heat shock daily beginning 4 days after egg laying. The rescued pho¹/pho¹ males are fully fertile, but occasionally show sex comb bristles on the mesothoracic legs and excess pigmentation in the 4th abdominal tergite. The rescued pho¹/pho¹ females have severely impaired fertility that cannot be rescued with a paternal pho⁺ allele. Only 10% of embryos derived from pho¹/pho¹ females crossed to pho¹/pho¹ males hatch, even when heat shocked before blastoderm to express pho^{hs.T:Ecol\lexA}. These embryos show frequent and various segmental abnormalities but never show classical homeotic derepression phenotypes. The few larvae that hatch grow slowly and never reach the pupal stage, probably because evident head skeleton abnormalities preclude normal feeding.

(Poux et al., 2001)

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

6212

pho¹/In(4)ci^D, ci^D pan^ciD

16111

y¹ w¹¹¹⁸; PBac{5HPw⁺}Syt7^A426/pho¹

Kyoto

103320

ci^D / pho¹

Notes on Origin

Discoverer

Isolated from: Bountiful, UT population.

(Hochman et al., 1964)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

(Hochman, 1976, Hochman, 1974, Hochman, 1971)

Pho¹

(Bengani et al., 2013)

l(4)102EFc¹

(Lindsley and Zimm, 1992)

l(4)29

(Lindsley and Zimm, 1992)

l(4)BU-2

(Hochman et al., 1964)

pho^1(l(4)29)

(Rusch and Kaufman, 2000)

pho¹

(Elizarev et al., 2021, Domsch et al., 2019, De et al., 2016, Steffen et al., 2013, Ji et al., 2012, Langlais et al., 2012, Brown and Kassis, 2010, Fujioka et al., 2008, González et al., 2008, Goodliffe et al., 2007, Klymenko et al., 2006, Bejarano et al., 2005, Hirose et al., 2001)

Name Synonyms

Secondary FlyBase IDs

References (36)

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