FlyBase Allele Report: Dmel\InR[93Dj-4]

General Information

Symbol

Dmel\InR^93Dj-4

Species

D. melanogaster

Name

FlyBase ID

FBal0010755

Feature type

allele

Associated gene

Dmel\InR

Associated Insertion(s)

Carried in Construct

Also Known As

InR^GC25, GC25, InR^93Dj4

Key Links

Allele class

Mutagen

gamma ray

Nature of the Allele

Allele class

Mutagen

gamma ray

(Mohler and Pardue, 1984)

Progenitor genotype

Caused by aberration

In(3R)GC25

Cytology

Description

Inversion breakpoint 5kb to 7kb upstream of the start of transcription.

(Chen et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of diabetic neuropathy

CEA with InR^E19

(Wang et al., 2023)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | dominant | prepupal stage

(Green and Extavour, 2012)

abnormal cell number | first instar larval stage (with InR^E19)

(Green and Extavour, 2012)

abnormal cell size | dominant | prepupal stage

(Green and Extavour, 2012)

abnormal cell size | prepupal stage (with InR^E19)

(Green and Extavour, 2012)

abnormal developmental rate | third instar larval stage | heat sensitive (with InR^E19)

(Shingleton et al., 2005)

abnormal pain response | larval stage (with InR^E19)

(Wang et al., 2023)

abnormal size | heat sensitive (with InR^E19)

(Shingleton et al., 2005)

chemical sensitive | dominant

(McClure et al., 2011)

decreased body size (with InR^E19)

(Clancy et al., 2001, Tatar et al., 2001)

decreased body size | adult stage (with InR^sP1)

(Neuman and Bashirullah, 2018)

decreased body size | heat sensitive (with InR^E19)

(Shingleton et al., 2005)

decreased body weight | pupal stage (with InR^E19)

(Kapali et al., 2022)

decreased cell number | heat sensitive (with InR^E19)

(Shingleton et al., 2005)

female sterile (with InR^E19)

(Tatar et al., 2001)

lethal | heat sensitive (with InR^E19)

(Shingleton et al., 2005)

lethal | recessive

(Chen et al., 1996, Mohler and Pardue, 1984)

lethal - all die before end of larval stage (with InR³⁰⁴)

(Oldham et al., 2002)

partially lethal - majority die (with InR^sP1)

(Neuman and Bashirullah, 2018)

short lived (with InR^E19)

(Tatar et al., 2001)

some die during larval stage (with InR³⁰⁴)

(Oldham et al., 2002)

viable (with InR^E19)

(Clancy et al., 2001)

visible

(Chen et al., 1996)

Phenotype Manifest In

Detailed Description

Statement

Reference

InR^93Dj-4/InR^93Dj-4 pupae are small compared to controls; but unlike controls, their size is not affected by hypoxia (10% O[[2]]).

(Kapali et al., 2022)

InR^E19/InR^93Dj-4 mutant embryos contain a reduced number of somatic gonad precursor (SGP) cell number at hatching (L0) compared to wild type. Transheterozygotes contain significantly fewer terminal filaments (TFs) and TF cells at the larval-pupal transition stage compared to controls, and TF cell size is also reduced. While each of the heterozygotes displays reduced SGP cell number and TF cell size, neither displays differences in either TF number or TF cell number.

Swarm cell migration is unaffected in InR^E19/InR^93Dj-4 mutants, and the proportion of cells anterior/posterior to the germline is also similar. However, the proportion of anterior cells that differentiate into TF cells is elevated in InR^E19/InR^93Dj-4 larvae compared to controls.

(Green and Extavour, 2012)

Heterozygous InR^93Dj-4 mutant flies reared on food containing 7.5% ethanol exhibit reduced adult viability compared to controls. Viability is comparable with controls when flies are raised on conventional media.

(McClure et al., 2011)

InR^E19/InR^93Dj-4 transheterozygotes show a temperature sensitive growth phenotype. The wing size of InR^E19/InR^93Dj-4 transheterozygotes is similar to that of wild-type controls when flies are raised at 18^oC. However, when flies are raised at 25^oC, the wing area of InR^E19/InR^93Dj-4 is much smaller than that of controls. Additionally, very few InR^E19/InR^93Dj-4 flies survive to adulthood when raised at 25^oC and none survive when raised at 29^oC.

The adult wing area of InR^E19/InR^93Dj-4 flies is only sensitive to temperature during late third instar and early pupation. Switching InR^E19/InR^93Dj-4 flies from 17^oC to 24^oC changes adult wing size between day 9 and 20 with earlier shifts resulting in smaller wings. The difference in wing size between flies raised at 17^oC and 24^oC is due to fewer, but not smaller, cells.

Total developmental time for InR^E19/InR^93Dj-4 transheterozygotes is sensitive to temperature only before the middle of the third larval instar. Switching InR^E19/InR^93Dj-4 flies from 17^oC to 24^oC changes the time to reach adult eclosion during the first 9 days of development with earlier switches causing greater delays in eclosion. A switch after the ninth day does not delay adult eclosion. At 17^oC, InR^E19/InR^93Dj-4 flies show a slight delay in eclosion relative to controls.

The adult body mass of InR^E19/InR^93Dj-4 flies is sensitive to a temperature change from 17^oC to 24^oC between day 9 and day 13. Shifting flies to the restrictive temperature after pupariation has no effect on adult body mass.

InR^E19/InR^93Dj-4 flies reared at 17^oC up to pupariation then switched to 24^oC results in an approximate doubling of free-sugar concentration compared to flies that are not switched to 24^oC. Lipid levels, however, are elevated in InR^E19/InR^93Dj-4 flies whether or not they are raised at the restrictive temperature either after pupariation or for the whole of their development time.

InR^E19/InR^93Dj-4 male flies raised at 17^oC show a reduction in the size of the maxillary palp, wing, and genital arch compared to wild-type flies. The size of the maxillary palp and wing is further reduced when these males are raised at 24^oC, but the genital arch size is not further decreased by the restrictive temperature.

(Shingleton et al., 2005)

InR^93Dj-4/InR^E19 adults are dwarf in size and are short-lived.

(Clancy et al., 2001)

InR^93Dj-4/InR^E19 adults are dwarf in size and are short-lived; life expectancy of both males and females is reduced compared to controls (from 32.3 to 1.7 and from 35.5 to 1.7 days respectively).

(Tatar et al., 2001)

Heterozygous adults have slightly reduced body size. Many InR^93Dj-4/InR^E19 animals survive to adulthood. They eclose with a delay of 8 to 9 days at 25^oC, having prolonged second and third larval instar stages compared to control siblings. The number of cells in the wing, eye-antennal, haltere, mesothoracic leg and metathoracic leg discs is reduced compared to wild-type. Pupae and adult flies are reduced in size compared to wild-type. InR^93Dj-4/InR^E19 males and females are fertile. Ovaries of InR^93Dj-4/InR^E19 females are reduced in size due to the small size of many underdeveloped ovarioles. The rate of ovariole maturation is slower than wild-type.

(Chen et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

InR^93Dj-4/InR³⁰⁴ has lethal | larval stage phenotype, suppressible | partially by Pten^2L100/Pten¹¹⁷

(Oldham et al., 2002)

Enhancer of

Statement

Reference

InR[+]/InR^93Dj-4 is an enhancer of increased cell number | adult stage phenotype of sl²

(Murillo-Maldonado et al., 2011)

InR[+]/InR^93Dj-4 is an enhancer of increased cell number | adult stage phenotype of sl¹

(Murillo-Maldonado et al., 2011)

NOT Enhancer of

Statement

Reference

InR[+]/InR^93Dj-4 is a non-enhancer of increased cell number | adult stage phenotype of sl⁹

(Murillo-Maldonado et al., 2011)

Other

Statement

Reference

InR^93Dj-4/InR³⁰⁴, Pten^2L100/Pten¹¹⁷ has lethal | pupal stage phenotype

(Oldham et al., 2002)

Phenotype Manifest In

Enhancer of

Statement

Reference

InR[+]/InR^93Dj-4 is an enhancer of photoreceptor cell R7 | adult stage phenotype of sl²

(Murillo-Maldonado et al., 2011)

InR[+]/InR^93Dj-4 is an enhancer of photoreceptor cell R7 | adult stage phenotype of sl¹

(Murillo-Maldonado et al., 2011)

NOT Enhancer of

Statement

Reference

InR[+]/InR^93Dj-4 is a non-enhancer of photoreceptor cell R7 | adult stage phenotype of sl⁹

(Murillo-Maldonado et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of InR^93Dj-4 enhances the percentage of sl² mutant ommatidia that contain extra R7 photoreceptors. The number of R7 cells per ommatidium is also increased.

One copy of InR^93Dj-4 enhances the increase in R7 photoreceptor cells per ommatidium seen in sl¹ mutant males.

One copy of InR^93Dj-4 does not enhance the increase in R7 photoreceptor cells per ommatidium that is seen in sl⁹ mutant males.

(Murillo-Maldonado et al., 2011)

for^R, InR^93Dj-4 double mutant flies show more sitter-like 'food-leaving' behaviour compared to for^R single mutants.

(Kent et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

InR^E19

(Chen et al., 1996)

Fails to complement

InR^E16

(Chen et al., 1996)

InR^E21

(Chen et al., 1996)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9554

In(3R)GC25, InR^93Dj-4/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

GC25

(Chen et al., 1996, Mohler and Pardue, 1984)

InR-GC25

(McClure et al., 2011)

InR²⁵

(Oldham et al., 2002)

InR^93Dj-4

(Banzai and Nishimura, 2023, Neuman and Bashirullah, 2018, Kent et al., 2009)

InR^93Dj4

(Wang et al., 2023, Luo et al., 2020)

InR^93dj-4

(Kent et al., 2009)

InR^G25

(Cong et al., 2015)

InR^GC25

(Kapali et al., 2022, Wei et al., 2016, McClure et al., 2011, Murillo-Maldonado et al., 2011, Shingleton et al., 2005, Tatar et al., 2001)

InRGC25

(Cong et al., 2015)

Inr^GC25

(Green and Extavour, 2012, Clancy et al., 2001)

l(3)93Dj⁴

l(3)er10^GC25

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

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