FlyBase Allele Report: Dmel\lncRNA:Hsrω[05241]

General Information

Symbol

Dmel\lncRNA:Hsrω⁰⁵²⁴¹

Species

D. melanogaster

Name

FlyBase ID

FBal0009533

Feature type

allele

Associated gene

Dmel\lncRNA:Hsrω

Associated Insertion(s)

P{PZ}lncRNA:Hsrω⁰⁵²⁴¹

Carried in Construct

Also Known As

hsrω⁰⁵²⁴¹

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Fernandez-Funez et al., 2000, Spradling, 1999.12.9, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{PZ}lncRNA:Hsrω⁰⁵²⁴¹

Cytology

Description

This insertion contains a 240bp deletion in the 5' UTR of the CG4408 gene.

(Verma et al., 2012)

P{PZ} insertion at -130bp in the promoter region of Hsrω.

(Lakhotia et al., 2001)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:21,296,502..21,296,502 [+]

(Verma et al., 2012, Mallik and Lakhotia, 2009, Akanksha et al., 2008, Sengupta and Lakhotia, 2006, Lakhotia et al., 2001, Rajendra et al., 2001, Fernandez-Funez et al., 2000, Spradling, 1999.12.9, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates neurodegenerative disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Sengupta and Lakhotia, 2006)

modeled by Hsap\HTT^Q93.ex1.UAS

(Sengupta and Lakhotia, 2006)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

male fertile

(Akanksha et al., 2008)

male fertile (with Df(3R)e-Gp4)

(Rajendra et al., 2001)

male fertile (with Df(3R)e-P)

(Rajendra et al., 2001)

male fertile (with Df(3R)GC14)

(Rajendra et al., 2001)

viable

(Lakhotia et al., 2001)

visible

(Sengupta and Lakhotia, 2006)

Phenotype Manifest In

rhabdomere

(Sengupta and Lakhotia, 2006)

Detailed Description

Statement

Reference

Homozygous males do not show any significant clustering of omega speckles in cyst cells of the testes.

(Akanksha et al., 2008)

FlyBase curator comment: the male sterile and omega speckle phenotypes described in FBrf0199067 as being due to the Hsrω⁰⁵²⁴¹ mutation have subsequently been found to be due to a second site mutation on the chromosome (ms(3)21²¹) and are not due to an effect on Hsrω (see FBrf0205520).

Eyes of Hsrω⁰⁵²⁴¹ homozygous flies appear normal externally. However, pseudopupil analysis of adult eyes reveals significant disorganisation of rhabdomeres in the majority of the ommatidial units. The ommatidia and axonal projections in Hsrω⁰⁵²⁴¹ homozygous mutants are organised as in wild-type.

(Sengupta and Lakhotia, 2006)

FlyBase curator comment: the male sterile phenotype described in FBrf0144961 as being due to the Hsrω⁰⁵²⁴¹ mutation has subsequently been found to be due to a second site mutation on the chromosome (ms(3)21²¹) and is not due to an effect on Hsrω (see FBrf0205520).

(Rajendra et al., 2001)

No obvious phenotype.

(Spradling et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

lncRNA:Hsrω⁰⁵²⁴¹/Hsromega[+] is an enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Akanksha et al., 2008, Sengupta and Lakhotia, 2006)

lncRNA:Hsrω⁰⁵²⁴¹/lncRNA:Hsrω⁰⁵²⁴¹ is an enhancer of abnormal neuroanatomy phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^GMR.PF

(Sengupta and Lakhotia, 2006)

lncRNA:Hsrω⁰⁵²⁴¹/lncRNA:Hsrω⁰⁵²⁴¹ is an enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Sengupta and Lakhotia, 2006)

Other

Statement

Reference

l(3)05241⁰⁵²⁴¹, lncRNA:Hsrω⁰⁵²⁴¹ has female sterile | germline clone phenotype

(Perrimon et al., 1996)

l(3)05241⁰⁵²⁴¹, lncRNA:Hsrω⁰⁵²⁴¹ has lethal | recessive | larval stage phenotype

(Perrimon et al., 1996)

Phenotype Manifest In

Enhancer of

Statement

Reference

lncRNA:Hsrω⁰⁵²⁴¹/Hsromega[+] is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Akanksha et al., 2008, Sengupta and Lakhotia, 2006)

lncRNA:Hsrω⁰⁵²⁴¹/lncRNA:Hsrω⁰⁵²⁴¹ is an enhancer of eye phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^GMR.PF

(Sengupta and Lakhotia, 2006)

lncRNA:Hsrω⁰⁵²⁴¹/lncRNA:Hsrω⁰⁵²⁴¹ is an enhancer of embryonic/larval optic stalk phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Sengupta and Lakhotia, 2006)

lncRNA:Hsrω⁰⁵²⁴¹ is an enhancer of eye phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

lncRNA:Hsrω⁰⁵²⁴¹ is an enhancer of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

Other

Statement

Reference

l(3)05241⁰⁵²⁴¹, lncRNA:Hsrω⁰⁵²⁴¹ has oocyte | germline clone phenotype

(Perrimon et al., 1996)

Additional Comments

Genetic Interactions

Statement

Reference

Germline clones homozygous for a 'l(3)05241' chromosome that carries both l(3)05241⁰⁵²⁴¹ and lncRNA:Hsrω⁰⁵²⁴¹ produce abnormal eggs due to abnormal oogenesis: few eggs are laid, oocytes show vitellogenic phenotype. Lethality of the 'l(3)05241' chromosome carrying both l(3)05241⁰⁵²⁴¹ and lncRNA:Hsrω⁰⁵²⁴¹ occurs during the L1 and L2 stages.

(Perrimon et al., 1996)

Xenogenetic Interactions

Statement

Reference

The neurodegeneration phenotype caused by expression of Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF is enhanced by Hsrω⁰⁵²⁴¹/+.

(Akanksha et al., 2008)

The severity of the neurodegeneration phenotypes caused by expression of Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF are enhanced by Hsrω⁰⁵²⁴¹. In the adult eye, in a Hsrω⁰⁵²⁴¹/+ background, the eyes are significantly reduced in size and in some cases necrotic lesions are also seen. In the developing eye disc, in a Hsrω⁰⁵²⁴¹ homozygous background, a more severe disorganisation of the ommatidial units and their projections is seen; individual ommatidial cellular integrity is lost and axonal connections to the optic stalk appear highly irregular.

The eye degeneration phenotype caused by expression of Hsap\HD^{Q93.ex1p.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced by Hsrω⁰⁵²⁴¹/Hsrω⁰⁵²⁴¹.

(Sengupta and Lakhotia, 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11654

ry⁵⁰⁶ P{PZ}lncRNA:Hsrω⁰⁵²⁴¹, l(3)05241⁰⁵²⁴¹/TM3, ry^RK Sb¹ Ser¹

Notes on Origin

Discoverer

Separable from: ms(3)21²¹.

(Akanksha et al., 2008)

Separable from: l(3)05241⁰⁵²⁴¹.

(Akanksha et al., 2008)

The P{PZ}Hsrω⁰⁵²⁴¹ insertion in the promoter of Hsrω is separable from a mutation causing recessive lethality (l(3)05241⁰⁵²⁴¹) and also from the ms(3)21²¹ second site mutation.

(Akanksha et al., 2008)

In FBrf0144961, it was reported that the P{PZ}Hsrω⁰⁵²⁴¹ insertion caused male sterility and an excessive clustering of omega speckles in cyst cells in the testes. However, these phenotypes are in fact caused by the second site ms(3)21²¹ mutation and are not due to an effect on Hsrω.

(Akanksha et al., 2008)

Induced with: l(3)05241⁰⁵²⁴¹.

(Spradling, 1999.12.9, BDGP Project Members, 1994-1999)

Comments

Complements: mod(mdg4)⁰⁴¹⁴³ Complements: mod(mdg4)⁰⁷⁰³⁸ Complements: E2f⁰⁷¹⁷² Complements: mod(mdg4)^L3101 Complements: l(3)neo55¹

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

05241

(Lakhotia et al., 2001)

Hsr93D⁰⁵²⁴¹

Hsrω⁰⁵²⁴¹

(Mallik and Lakhotia, 2009)

Hst-ω⁰⁵²⁴¹

(Fernandez-Funez et al., 2000)

hsrω<up>05241

(Lakhotia et al., 1999)

hsrω⁰⁵²⁴¹

(Verma et al., 2012, Akanksha et al., 2008, Sengupta and Lakhotia, 2006, Rajendra et al., 2001)

hsrω^05241P*

(Akanksha et al., 2008)

l(3)05241

(Perrimon et al., 1996, BDGP Project Members, 1994-1999)

l(3)05241⁰⁵²⁴¹

lncRNA:Hsrω⁰⁵²⁴¹

n(3)05241

(Spradling, 1999.12.9, Spradling et al., 1999)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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