FlyBase Allele Report: Dmel\dap[04454]

General Information

Symbol

Dmel\dap⁰⁴⁴⁵⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0008062

Feature type

allele

Associated gene

Dmel\dap

Associated Insertion(s)

P{PZ}dap⁰⁴⁴⁵⁴

Carried in Construct

Also Known As

dap⁴⁴⁵⁴, l(2)04454

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Berger et al., 2005, de Nooij et al., 1996)

Mutagen

P-element activity

(de Nooij et al., 1996)

Progenitor genotype

Associated Insertion(s)

P{PZ}dap⁰⁴⁴⁵⁴

Cytology

Description

P{PZ} insertion within the 5' untranslated region.

(de Nooij et al., 1996)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:9,712,596..9,712,596 [+]

(Baumgardt et al., 2014, Dui et al., 2013, Spradling et al., 1999, de Nooij et al., 1996, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle

(de Nooij et al., 1996)

abnormal neuroanatomy

(Berger et al., 2005)

increased occurrence of cell division | embryonic stage

(Monedero Cobeta et al., 2017)

lethal (with Df(2R)BSC132)

(Kahsai et al., 2016)

lethal | recessive

(Spradling et al., 1999, de Nooij et al., 1996)

Phenotype Manifest In

embryo

(de Nooij et al., 1996)

embryonic/larval tracheal dorsal trunk

(Beitel and Krasnow, 2000)

embryonic/larval tracheal transverse connective

(Beitel and Krasnow, 2000)

embryonic abdomen & glial cell | ectopic

(Berger et al., 2005)

embryonic epidermis

(Shibutani et al., 2007)

embryonic longitudinal glial cell

(Griffiths and Hidalgo, 2004)

larval neuroblast | embryonic stage

(Monedero Cobeta et al., 2017)

ventral nerve cord primordium | embryonic stage

(Monedero Cobeta et al., 2017)

Detailed Description

Statement

Reference

dap⁰⁴⁴⁵⁴ mutant embryos show a significant increase in the mitotic index of neuroblast daughter cells, but not of neuroblasts, in the A6 to A10 ventral nerve cord segment, as compared to controls.

(Monedero Cobeta et al., 2017)

Homozygous embryos have an ectopic S₁₇ phase in the epidermis.

(Shibutani et al., 2007)

In dap⁰⁴⁴⁵⁴ null mutants an additional glial cell is observed in the NB6-4a lineage in approximately 66% of abdominal segments, although no neuron-like cells are observed. Thoracic segments are not affected.

(Berger et al., 2005)

Mutant stage 16 longitudinal glial cells are rounded (in contrast to wild-type longitudinal glia, which have spread out by this stage). Mutant longitudinal glial cells show an excess of mitosis at stage 15 (a time when no longitudinal glia divide in wild-type embryos).

(Griffiths and Hidalgo, 2004)

Some branches of the tracheal system have nearly twice the normal number of tracheal cells in mutant embryos. Despite the increased cell number, primary branching occurs normally and branch sizes do not increase.

(Beitel and Krasnow, 2000)

Homozygous embryos fail to arrest their cell cycles at cycle 17 and progress through another complete cell cycle. Embryos may contain as many as 70% more cells than wild-type (the number of additional cells shows considerable variation among individual embryos). dap^2X10/dap⁰⁴⁴⁵⁴ transheterozygous and dap⁰⁴⁴⁵⁴/Df(2R)B5 hemizygous combination is almost fully lethal.

(de Nooij et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

dap⁰⁴⁴⁵⁴ has increased occurrence of cell division | embryonic stage phenotype, enhanceable by Df(3L)ED225

(Monedero Cobeta et al., 2017)

NOT Enhancer of

Statement

Reference

dap⁰⁴⁴⁵⁴ is a non-enhancer of visible phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

dap⁰⁴⁴⁵⁴ is a non-enhancer of visible phenotype of Hsap\TP53^UAS.cYa, Scer\GAL4^GMR.PS

(Yamaguchi et al., 1999)

Suppressor of

Statement

Reference

dap[+]/dap⁰⁴⁴⁵⁴ is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^en-e16E, Skp2^KK108837

(Dui et al., 2013)

dap⁰⁴⁴⁵⁴ is a suppressor | partially of abnormal mitotic cell cycle | maternal effect phenotype of png¹⁰⁵⁸/png³³¹⁸

(Lee et al., 2001)

NOT Suppressor of

Statement

Reference

dap⁰⁴⁴⁵⁴ is a non-suppressor of visible phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

dap⁰⁴⁴⁵⁴ is a non-suppressor of visible phenotype of Hsap\TP53^UAS.cYa, Scer\GAL4^GMR.PS

(Yamaguchi et al., 1999)

Phenotype Manifest In

Enhanced by

Statement

Reference

dap⁰⁴⁴⁵⁴ has ventral nerve cord primordium | embryonic stage phenotype, enhanceable by Df(3L)ED225

(Monedero Cobeta et al., 2017)

dap⁰⁴⁴⁵⁴ has larval neuroblast | embryonic stage phenotype, enhanceable by Df(3L)ED225

(Monedero Cobeta et al., 2017)

NOT Enhancer of

Statement

Reference

dap⁰⁴⁴⁵⁴ is a non-enhancer of eye phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

Suppressor of

Statement

Reference

dap[+]/dap⁰⁴⁴⁵⁴ is a suppressor | partially of wing hair phenotype of Scer\GAL4^en-e16E, Skp2^KK108837

(Dui et al., 2013)

NOT Suppressor of

Statement

Reference

dap⁰⁴⁴⁵⁴ is a non-suppressor of eye phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

dap⁰⁴⁴⁵⁴, Df(3L)ED225 double mutants show a significant increase in the mitotic index of neuroblasts compared to either single mutant, but not an enhancement of the increased mitotic index of neuroblast daughter cells observed in dap⁰⁴⁴⁵⁴ single mutants.

(Monedero Cobeta et al., 2017)

One copy of dap⁰⁴⁴⁵⁴ partially suppresses the wing hair spacing phenotype seen when Skp2^KK108837 is expressed under the control of Scer\GAL4^en-e16E.

(Dui et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

dap^rQ447

(BDGP Project Members, 1994-1999)

dap^k07309

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11377

cn¹ P{PZ}dap⁰⁴⁴⁵⁴/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: Mmp2⁰²³⁵³. Complements: Uba1⁰³⁴⁰⁵. Complements: Uba1⁰³⁸⁷⁶. Complements: Mmp2⁰⁵⁸⁶⁵. Complements: l(2)k03111^k02003a. Complements: l(2)k03111^k03111. Complements: l(2)k06205^k06205. Complements: l(2)k09221^k09221.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

04454

(Mukherjee et al., 2006)

dacapo04454

(Griffiths and Hidalgo, 2004)

dacapo⁰⁴⁴⁵⁴

(Griffiths et al., 2007)

dacapo⁴⁴⁵⁴

(Crack et al., 2002)

dap⁰⁴⁴⁵⁴

(Otsuki and Brand, 2019, Yaghmaeian Salmani et al., 2018, Monedero Cobeta et al., 2017, Bivik et al., 2015, Baumgardt et al., 2014, Dui et al., 2013, Legent et al., 2006, Huet et al., 2002)

dap⁴⁴⁵⁴

(Shibutani et al., 2007, Swanhart et al., 2007, Berger et al., 2005, Frolov et al., 2003, Beitel and Krasnow, 2000, Yamaguchi et al., 1999, de Nooij et al., 1996)

l(2)04454

(Lee et al., 2001, Spradling et al., 1999)

l(2)04454⁰⁴⁴⁵⁴

Name Synonyms

Secondary FlyBase IDs

References (25)

Report Sections

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