FlyBase Allele Report: Dmel\eEF5[01296]

General Information

Symbol

Dmel\eEF5⁰¹²⁹⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0007969

Feature type

allele

Associated gene

Dmel\eEF5

Associated Insertion(s)

P{PZ}eEF5⁰¹²⁹⁶

Carried in Construct

Also Known As

l(2)01296

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{PZ}eEF5⁰¹²⁹⁶

Cytology

Description

P{PZ} insertion in intron 1 of the CT10685 transcript of eIF-5A, 493bp downstream of the 5' end of the transcript.

(Misra, 2000.8.25)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:24,058,335..24,058,335 [+]

(Misra, 2000.8.25, Spradling et al., 1999, Harvie et al., 1998, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell size

(Patel et al., 2009)

lethal | recessive

(Spradling et al., 1999, Harvie et al., 1998)

Phenotype Manifest In

embryonic/larval fat body

(Patel et al., 2009)

Detailed Description

Statement

Reference

Homozygous mutant eIF-5A⁰¹²⁹⁶ larvae exhibit a severe larval growth defect and are much smaller than wild-type larvae, although they live for 8 days.

eIF-5A⁰¹²⁹⁶ mutant larval fat bodies undergo constitutive autophagy under fed conditions.

(Patel et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

eEF5⁰¹²⁹⁶ has decreased cell size phenotype, enhanceable by nero¹

(Patel et al., 2009)

Suppressor of

Statement

Reference

eEF5⁰¹²⁹⁶ is a suppressor | partially of abnormal mitotic cell cycle | maternal effect phenotype of png¹⁰⁵⁸/png³³¹⁸

(Lee et al., 2001)

eEF5⁰¹²⁹⁶/eIF-5A[+] is a suppressor of abnormal mitotic cell cycle | maternal effect phenotype of png¹⁰⁵⁸

(Lee et al., 2001)

Phenotype Manifest In

Enhancer of

Statement

Reference

eEF5⁰¹²⁹⁶/eIF-5A[+] is an enhancer of wing margin phenotype of Bx²

(Bronstein et al., 2010)

NOT Enhancer of

Statement

Reference

eEF5⁰¹²⁹⁶/eIF-5A[+] is a non-enhancer of scutellar bristle | increased number phenotype of Chi^e5.5

(Bronstein et al., 2010)

eEF5⁰¹²⁹⁶/eIF-5A[+] is a non-enhancer of scutellar bristle | increased number phenotype of Ssdp^L7

(Bronstein et al., 2010)

NOT Suppressor of

Statement

Reference

eEF5⁰¹²⁹⁶/eIF-5A[+] is a non-suppressor of scutellar bristle | increased number phenotype of Chi^e5.5

(Bronstein et al., 2010)

eEF5⁰¹²⁹⁶/eIF-5A[+] is a non-suppressor of scutellar bristle | increased number phenotype of Ssdp^L7

(Bronstein et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

eIF-5A⁰¹²⁹⁶; nero¹ double mutant larvae display a slightly more severe larval growth defect than eIF-5A⁰¹²⁹⁶ mutants alone.

(Patel et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

eEF5¹⁰⁵³⁰

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11039

cn¹ P{PZ}eEF5⁰¹²⁹⁶/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: tsr⁰⁰⁰²⁶. Complements: ken⁰⁰⁶²⁸. Complements: l(2)05006⁰⁵⁰⁰⁶. Complements: gek⁰⁹³⁷³. Complements: tsr^k00508. Complements: tsr^k01208. Complements: esg^k03012a. Complements: tsr^k03101. Complements: Adk2-60B^k04209. Complements: Chi^k04405. Complements: crp^k05211a. Complements: tsr^k05403. Complements: l(2)k05419a^k05419a. Complements: tsr^k05633. Complements: Nop60B^k06308. Complements: l(2)k06608b^k06608b. Complements: Phm^k07623. Complements: tsr^k09317. Complements: l(2)rQ313^rQ313.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

eEF5⁰¹²⁹⁶

eIF-5A⁰¹²⁹⁶

(Bronstein et al., 2010)

eIF5A^P01296

(Patel et al., 2009)

l(2)01296

(Lee et al., 2001, Misra, 2000.8.25, Spradling et al., 1999)

l(2)01296⁰¹²⁹⁶

(BDGP Project Members, 1994-1999)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

Open Close