The frequency of leg-to-wing transdetermination caused by expression of wg^Act5C.PS in clones during the mid-second larval instar is dominantly suppressed by jing⁰¹⁰⁹⁴.

jing⁰¹⁰⁹⁴/spi¹ double heterozygous embryos have a reduced number of midline glia per ventral nerve cord segment compared to wild type. S^IIN/jing⁰¹⁰⁹⁴ double heterozygous embryos have breaks in the dorsal trunk of the tracheal system at stage 15.

In sim², jing⁰¹⁰⁹⁴ double heterozygotes, the axon formation in the embryonic central nervous system (CNS) and midline cell development are perturbed. Over half of embryos exhibit a 'stalled axon' phenotype. About 8% exhibit a 'collapsed axon' phenotype. The midline cells are also displaced ventrally in these embryos. In almost 40% tgo¹, jing⁰¹⁰⁹⁴ double homozygotes fused commissures are seen. 2/3 of tgo¹, jing⁰¹⁰⁹⁴, sim² triple heterozygotes exhibit a 'collapsed axon' phenotype, about 10% have fused commissures. The midline cells are also displaced dorsally and ventrally in these embryos. Half of jing⁰¹⁰⁹⁴, sli¹ double homozygous embryos exhibit a 'collapsed axon' phenotype, about a third a have fused commissures. Midline cells are displaced ventrally in these embryos. trh¹, jing⁰¹⁰⁹⁴ double heterozygous embryos display a loss of about half of their trachea. When tgo¹ is also added, 69% of embryos display a loss of about half of their trachea. jing⁰¹⁰⁹⁴, tgo¹ double heterozygous embryos display a loss of about 1/5 of their trachea. jing⁰¹⁰⁹⁴, btl^H82Δ3 double heterozygotes show an almost complete loss of trachea.