FlyBase Allele Report: Dmel\tgo[1]

General Information

Symbol

Dmel\tgo¹

Species

D. melanogaster

Name

FlyBase ID

FBal0082188

Feature type

allele

Associated gene

Dmel\tgo

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Sonnenfeld et al., 1997)

Mutagen

ethyl methanesulfonate

(Sonnenfeld et al., 1997)

Progenitor genotype

Cytology

Description

Termination codon at amino acid 532 that deletes a Pro-rich region, the PRD repeat and a Gln-rich region.

(Sonnenfeld et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:9,018,093..9,018,093 [-]

Nucleotide change:

T9018093R

Amino acid change:

Y530term | tgo-PA; Y530term | tgo-PB

Reported amino acid change:

Y530term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Thanawala et al., 2013, Sonnenfeld et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration | oogenesis stage S9 | somatic clone

(Doronkin et al., 2010)

abnormal cell migration | oogenesis stage S10 | somatic clone

(Doronkin et al., 2010)

lethal | recessive

(Sonnenfeld et al., 1997)

visible | recessive | somatic clone

(Emmons et al., 1999)

Phenotype Manifest In

antenna | somatic clone

(Emmons et al., 1999)

arista | somatic clone

(Emmons et al., 1999)

axon

(Sonnenfeld et al., 1997)

basal cylinder | somatic clone

(Emmons et al., 1999)

border follicle cell | oogenesis stage S9 | somatic clone

(Doronkin et al., 2010)

border follicle cell | oogenesis stage S10 | somatic clone

(Doronkin et al., 2010)

embryonic/larval tracheal system

(Sonnenfeld et al., 1997)

larval VUM neuron

(Sonnenfeld et al., 1997)

leg | distal | somatic clone

(Emmons et al., 1999)

macrochaeta | somatic clone

(Emmons et al., 1999)

maxillary palp | somatic clone

(Emmons et al., 1999)

midline glial cell

(Sonnenfeld et al., 1997)

neuroblast MNB

(Sonnenfeld et al., 1997)

pretarsus | somatic clone

(Emmons et al., 1999)

tarsal segment 4 | somatic clone

(Emmons et al., 1999)

tarsal segment | somatic clone

(Emmons et al., 1999)

third segment of antenna | distal | somatic clone

(Emmons et al., 1999)

unguis | ectopic | somatic clone

(Emmons et al., 1999)

wing | somatic clone

(Emmons et al., 1999)

Detailed Description

Statement

Reference

During stage 9 of oogenesis, 80-90% of border cell clusters with tgo¹-mutant cells migrate faster than the corresponding anterior follicle cells, reaching the nurse cell and oocyte border prematurely. In contrast, the predominant phenotype observed during stage 10 is a delay in migration.

(Doronkin et al., 2010)

Intermediate allele. Homozygous clones have no effect in the proximal antennal segments (segments 1 and 2). However, they transform the distal part of antennal segment 3, the basal cylinder and the arista to a well-segmented tarsus that usually has a partially formed claw. The proximal part of antennal segment 3 is unaffected. Homozygous clones in the leg can show weak pattern deletions. Defects include shortening of tarsal segments and a partial fusion of tarsal segments 4 and 5. Clones in the proximal part of the leg have no effect. Homozygous clones in the maxillary palps result in weak deletions in the palp. Homozygous clones in the wing hinge region cause the wing to be held out away from the body. Homozygous clones have an intermediate effect on bristle size compared to tgo⁵ clones.

(Emmons et al., 1999)

Reduction in the number of midline glia to 1 per segment. The number of VUM neurons and MNB progeny are reduced to 60% of wild type and do not migrate into the ventral regions of the VNC. Flies exhibit severe defects in tracheal tubule formation. Some embryos show a mild defect in axon morphology.

(Sonnenfeld et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

tgo¹/tgo[+] is an enhancer of tracheal section phenotype of jing[+]/jing⁰¹⁰⁹⁴, trh¹

(Sedaghat et al., 2002)

tgo¹/tgo[+] is an enhancer of symmetrical commissure phenotype of jing⁰¹⁰⁹⁴

(Sedaghat et al., 2002)

tgo¹/tgo[+] is an enhancer of tracheal section phenotype of jing⁰¹⁰⁹⁴, trh¹/trh[+]

(Sedaghat et al., 2002)

tgo¹/tgo[+] is an enhancer of presumptive embryonic/larval central nervous system phenotype of jing⁰¹⁰⁹⁴, sim²/sim[+]

(Sedaghat et al., 2002)

tgo¹/tgo[+] is an enhancer of presumptive embryonic/larval central nervous system phenotype of jing[+]/jing⁰¹⁰⁹⁴, sim²

(Sedaghat et al., 2002)

Other

Statement

Reference

btl^H82Δ3, tgo¹, trh¹ has tracheal section phenotype

(Sedaghat et al., 2002)

jing⁰¹⁰⁹⁴, tgo¹/tgo[+] has tracheal section phenotype

(Sedaghat et al., 2002)

jing⁰¹⁰⁹⁴, tgo¹ has tracheal section phenotype

(Sedaghat et al., 2002)

jing⁰¹⁰⁹⁴, sim²/sim[+], tgo¹/tgo[+] has ventral midline of embryo phenotype

(Sedaghat et al., 2002)

jing[+]/jing⁰¹⁰⁹⁴, sim², tgo¹/tgo[+] has ventral midline of embryo phenotype

(Sedaghat et al., 2002)

jing⁰¹⁰⁹⁴, sim²/sim[+], tgo¹ has ventral midline of embryo phenotype

(Sedaghat et al., 2002)

sim², tgo¹ has central nervous system phenotype

(Sonnenfeld et al., 1997)

tgo¹, trh⁸ has embryonic/larval tracheal system phenotype

(Sonnenfeld et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

In almost 40% tgo¹, jing⁰¹⁰⁹⁴ double homozygotes fused commissures are seen. 2/3 of tgo¹, jing⁰¹⁰⁹⁴, sim² triple heterozygotes exhibit a 'collapsed axon' phenotype, about 10% have fused commissures. The midline cells are also displaced dorsally and ventrally in these embryos. jing⁰¹⁰⁹⁴, tgo¹ double heterozygous embryos display a loss of about 1/5 of their trachea. When trh¹ is also added, 69% of embryos display a loss of about half of their trachea.