FB2024_03 , released June 25, 2024
Allele: Dmel\ash114
Open Close
General Information
Symbol
Dmel\ash114
Species
D. melanogaster
Name
FlyBase ID
FBal0000746
Feature type
allele
Associated gene
Dmel\ash1
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

heat sensitive hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

heat sensitive hypomorphic allele - genetic evidence

(Tripoulas et al., 1996)
Mutagen
ethyl methanesulfonate
(Tripoulas et al., 1996, Tripoulas et al., 1994)
Progenitor genotype
Cytology
Description

Mutation alters the consensus 5' splice site of the second intron from GT to GA. If no splicing occurs, then because of stop codons in the intron, the protein product would terminate after 2001 amino acids and would be missing the C terminal 143 amino acids.

(Tripoulas et al., 1996)

Nucleotide substitution: T7127A.

(Tripoulas et al., 1996)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:19,591,816..19,591,816 [-]
Nucleotide change:

T19591816A

Reported nucleotide change:

T7127A

Comment:

T to A alteration in consensus splice donor site.

(Tripoulas et al., 1996)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      ash114/Df(3L)kto2 lethality occurs during late pupal or pharate stages.

      (Tripoulas et al., 1996)

      Significant viability when raised at 20oC, with reduced viability at higher temperatures. Temperature-sensitive period for viability is primarily during the third larval instar. Exposure to 29oC for as little as one day during the third larval instar causes a high frequency of homeotic transformations of the third thoracic segment to the second thoracic segment with no significant loss of viability. ash11/ash114 or ash114/ash128 females show a reversible block in oogenesis at 27oC: an increasing number of stage 14 egg chambers is evident per ovary with increasing temperature. Pole cell transplantation reveals that embryogenesis requires germ line autonomous, maternal expression of ash1.

      (Tripoulas et al., 1994)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Suppressor of
      Statement
      Reference

      ash114 is a non-suppressor of wing phenotype of Scer\GAL4OK386, nejUAS.cMa

      (Bantignies et al., 2000)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Double heterozygotes with trxel2 or brm2 exhibit transformation of third leg to second leg identity and haltere to wing transformation.

      (Tripoulas et al., 1996)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Range of lethality for transheterozygotes extends from the third larval instar for the most severe alleles to the pharate adult stage for the least severe alleles.

      (Tripoulas et al., 1994)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      VD234
      ash114
      (Tripoulas et al., 1996)
      dash14
      (Lindsley and Zimm, 1992)
      dashV234
      (Shearn et al., 1971)
      Name Synonyms
      Secondary FlyBase IDs
        References (6)