FlyBase Allele Report: Dmel\ash1[1]

General Information

Symbol

Dmel\ash1¹

Species

D. melanogaster

Name

FlyBase ID

FBal0000731

Feature type

allele

Associated gene

Dmel\ash1

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

ICR 170

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Tripoulas et al., 1996, LaJeunesse and Shearn, 1995)

Mutagen

ICR 170

(Tripoulas et al., 1996, LaJeunesse and Shearn, 1995, Tripoulas et al., 1994)

Progenitor genotype

Cytology

Description

Insertion of a C after nucleotide 4108, causing a frame shift at amino acid residue 1032.

(Tripoulas et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Tripoulas et al., 1996, LaJeunesse and Shearn, 1995, Tripoulas et al., 1994)

visible | homeotic

(Ragab, 2006, Tripoulas et al., 1996, Tripoulas et al., 1994, Shearn, 1989)

Phenotype Manifest In

haltere

(Ragab, 2006, Shearn, 1989)

ovary | heat sensitive (with ash1¹⁴)

(Tripoulas et al., 1994)

wing

(Shearn, 1989)

wing | ectopic

(Ragab, 2006)

Detailed Description

Statement

Reference

ash1¹ flies show a haltere to wing transformation.

(Ragab, 2006)

Lethality occurs during the larval and pupal stages. Double heterozygotes with Ubx^abx-1, trx^el2 or brm² exhibit transformation of third leg to second leg identity and haltere to wing transformation.

(Tripoulas et al., 1996)

ash1¹/ash1¹⁴ females show a reversible block in oogenesis at 27^oC: an increasing number of stage 14 egg chambers is evident per ovary with increasing temperature.

(Tripoulas et al., 1994)

50% penetrance of the posterior wing to anterior wing transformation.

(Shearn, 1989)

The frequency of variegated bithorax transformations seen in Df(3R)red-P52 heterozygotes is increased in flies also containing a copy of ash1¹.

(Capdevila and Garcia-Bellido, 1981)

Lethality acts at the prepupal/pupal interface.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

ash1¹ has visible | homeotic phenotype, non-suppressible by Df(2R)ED3921

(Ragab, 2006)

Other

Statement

Reference

ash1[+]/ash1¹, brm⁶ has visible | homeotic phenotype

(Tripoulas et al., 1994)

ash1[+]/ash1¹, brm² has visible | homeotic phenotype

(Tripoulas et al., 1994)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

ash1¹ has haltere phenotype, non-suppressible by Df(2R)ED3921

(Ragab, 2006)

ash1¹ has wing | ectopic phenotype, non-suppressible by Df(2R)ED3921

(Ragab, 2006)

Enhancer of

Statement

Reference

ash2¹/ash1¹ is an enhancer of adult abdominal segment 4 | ectopic phenotype of E(z)^Trm

(Bajusz et al., 2001)

ash2¹/ash1¹ is an enhancer of adult abdominal segment 5 phenotype of E(z)^Trm

(Bajusz et al., 2001)

ash2¹/ash1¹ is an enhancer of adult mesothoracic segment | ectopic phenotype of E(z)^Trm

(Bajusz et al., 2001)

ash2¹/ash1¹ is an enhancer of adult metathoracic segment phenotype of E(z)^Trm

(Bajusz et al., 2001)

ash2¹/ash1¹ is an enhancer of adult prothoracic segment phenotype of E(z)^Trm

(Bajusz et al., 2001)

Suppressor of

Statement

Reference

ash1¹ is a suppressor of wing phenotype of Scer\GAL4^OK386, nej^UAS.cMa

(Bantignies et al., 2000)

Other

Statement

Reference

ash1¹, brm[+]/brm² has scutellum phenotype

(Tripoulas et al., 1994)

ash1¹, brm[+]/brm² has metathoracic leg phenotype

(Tripoulas et al., 1994)

ash1[+]/ash1¹, brm⁶ has scutellum phenotype

(Tripoulas et al., 1994)

ash1[+]/ash1¹, brm⁶ has metathoracic leg phenotype

(Tripoulas et al., 1994)

ash1[+]/ash1¹, brm⁶ has haltere phenotype

(Tripoulas et al., 1994)

ash1[+]/ash1¹, brm⁶ has prothoracic leg phenotype

(Tripoulas et al., 1994)

ash1¹, brm⁵/brm[+] has metathoracic leg phenotype

(Tripoulas et al., 1994)

ash1¹, brm[+]/brm² has haltere phenotype

(Tripoulas et al., 1994)

ash1¹, brm[+]/brm² has prothoracic leg phenotype

(Tripoulas et al., 1994)

ash1[+]/ash1¹, brm² has scutellum phenotype

(Tripoulas et al., 1994)

ash1[+]/ash1¹, brm² has metathoracic leg phenotype

(Tripoulas et al., 1994)

ash1[+]/ash1¹, brm² has haltere phenotype

(Tripoulas et al., 1994)

ash1[+]/ash1¹, brm² has prothoracic leg phenotype

(Tripoulas et al., 1994)

Additional Comments

Genetic Interactions

Statement

Reference

Df(2R)ED3921 fails to suppress the haltere to wing transformation of ash1¹ flies.

(Ragab, 2006)

Heterozygotes of ash1¹, ash1⁶, and ash1²⁸ with brm² have homeotic transformations of the third to the second leg. The expressivity of the transformed leg is variable, some have only a second leg apical bristle while others have a second leg preapical bristle as well. There is a lower penetrance of third leg transformations among flies heterozygous for ash1¹, ash1⁶, and ash1²⁸ with brm⁵. Degree of penetrance corresponds to the severity of the ash1 allele (ash1¹ < ash1⁶ < ash1²⁸). There is a lower penetrance of partial homeotic transformation of the haltere to the wing, or dorsal metathoracic cuticle to mesothoracic cuticle, and an even lower penetrance of first leg to second leg transformations.

(Tripoulas et al., 1994)

Flies heterozygous for both Df(3R)red-P93 and ash1¹ express a weak transformation of halteres to wings or third legs to second legs.

(Shearn, 1989)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Range of lethality for transheterozygotes extends from the third larval instar for the most severe alleles to the pharate adult stage for the least severe alleles.

(Tripoulas et al., 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

III-10

ash-1^III-10

(Shearn, 1989)

ash1¹

ash¹

(Bengani et al., 2013)

dash¹

(Shearn et al., 1971)

l(3)III-10

(Capdevila and Garcia-Bellido, 1981)

l(3)SG29a¹

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

Open Close