FB2024_04 , released June 25, 2024
Aberration: Dmel\Df(2L)Prl
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General Information
Symbol
Df(2L)Prl
Species
D. melanogaster
Name
Deficiency (2L) Proxless
FlyBase ID
FBab0001522
Feature type
chromosomal_deletion
Also Known As
Df(2L)Pr1
Computed Breakpoints include

32F1-32F3;33F1-33F2

Features within 32F1--33F2
Genomic Maps
Sequence coordinates
2L:11,437,223..11,497,070 (Df(2L)Prl:bk1)
(Yamamoto et al., 2009.2.25)
2L:12,614,220..12,626,238 (Df(2L)Prl:bk2)
(Yamamoto et al., 2009.2.25)
Member of large scale dataset(s)
Nature of Aberration
Cytological Order
Progenitor
Mutagen
Class of aberration (relative to wild type)
chromosomal_deletion
(FlyBase, 2007)
Class of aberration (relative to progenitor)
chromosomal_deletion
(Bahri et al., 1997, Duffy et al., 1996, Musacchio and Perrimon, 1996, Harbecke and Lengyel, 1995, Pauli et al., 1995, Landecker et al., 1994)
Breakpoints

32F1-32F3;33F1-33F2

(Duffy et al., 1996, Musacchio and Perrimon, 1996, Harbecke and Lengyel, 1995, Pauli et al., 1995, Landecker et al., 1994)

[];[]

(Bahri et al., 1997)
Causes alleles
nubPrl
(Yamamoto et al., 2009.2.25, Ng et al., 1995, Yeo et al., 1995)
Prl1
(Carpenter, 1993.8.8)
Carries alleles
Transposon Insertions
Formalized genetic data

RpS13 << bk1 << Prl << bk2 << pdm2

Genetic mapping information
Comments

This deletion removes a single ribosomal protein-coding gene (though other genes are also removed).

(Marygold et al., 2007)

Breakpoint(s) molecularly mapped

(Frei et al., 1988)

Distal breakpoint mapped to the DNA at about -430 to -400 kb (Frei et al., 1988).

Comments on Cytology

The left Df(2L)Prl breakpoint lies in salm, sala or CG6488 or in a region between the genes, and lies in the range 2L:11437223..11497070 (R5) (predicted cytology: 32F1-2).

(Yamamoto et al., 2009.2.25)

The right Df(2L)Prl breakpoint lies within nub proximal to or within ref2, and lies in the range 2L:12614220..12626238 (R5) (predicted cytology: 33F1-2).

(Yamamoto et al., 2009.2.25)

Limits of break 1 from polytene analysis (FBrf0076124) Left limit of break 2 from inclusion of kek1 (FBrf0089758) Right limit of break 2 from polytene analysis (FBrf0076124)

Sequence Crossreferences
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
Gene Deletion and Duplication Data
Genes Deleted / Disrupted
Complementation Data
Completely deleted / disrupted
bru1
(Spradling et al., 1999, BDGP Project Members, 1994-1999, Schupbach and Wieschaus, 1991)
bun
(Gluderer et al., 2008, Spradling et al., 1999, BDGP Project Members, 1994-1999)
cana
(Spradling et al., 1999)
Ca-β
(Spradling et al., 1999, BDGP Project Members, 1994-1999)
cmet
(Spradling et al., 1999, BDGP Project Members, 1994-1999)
crol
(Spradling et al., 1999, BDGP Project Members, 1994-1999)
dmGlut
(Spradling et al., 1999, BDGP Project Members, 1994-1999)
eRF3
(Harris et al., 2003, Spradling et al., 1999, Basu et al., 1998, BDGP Project Members, 1994-1999)
esc
(Jurgens, 1988)
kek1
(Musacchio and Perrimon, 1996)
l(2)04518b
(Spradling et al., 1999, BDGP Project Members, 1994-1999)
l(2)08307a
(Spradling et al., 1999, BDGP Project Members, 1994-1999)
Mal-B1
(Parker and Roberts, 1996)
Mal-B2
(Parker and Roberts, 1996)
MESK2
(BDGP Project Members, 1994-1999)
nub
(Cifuentes and Garcia-Bellido, 1997, Musacchio and Perrimon, 1996, Yeo et al., 1995, Bhat and Schedl, 1994)
Nup154
(Spradling et al., 1999, BDGP Project Members, 1994-1999)
Pkd2
(Gao et al., 2004)
prd
(Harbecke and Lengyel, 1995, Yeo et al., 1995, Jurgens, 1988, Nusslein-Volhard et al., 1984)
Rab6
(Spradling et al., 1999, BDGP Project Members, 1994-1999)
rdgH
(Colley, 2016.6.20)
sala
(Frei et al., 1988, Nusslein-Volhard et al., 1984)
salm
(Barrio et al., 1999, Spradling et al., 1999, Harbecke and Lengyel, 1995, BDGP Project Members, 1994-1999, Jurgens, 1988, Tearle and Nusslein-Volhard, 1987, Nusslein-Volhard et al., 1984)
ThrRS
(Spradling et al., 1999, BDGP Project Members, 1994-1999)
zuc
(Chen et al., 2021, Schupbach and Wieschaus, 1991)
Partially deleted / disrupted
Molecular Data
Completely deleted
RpL7-like
(Marygold et al., 2007)
Partially deleted
nub
(Yamamoto et al., 2009.2.25)
Genes NOT Deleted / Disrupted
Genes Duplicated
Complementation Data
Completely duplicated
Partially duplicated
Molecular Data
Completely duplicated
Partially duplicated
Genes NOT Duplicated
Complementation Data
 
Molecular Data
 
Affected Genes Inferred by Location
Phenotypic Data
In combination with other aberrations

No effect on In(1)wm4h position-effect variegation.

(Wustmann et al., 1989)
NOT in combination with other aberrations

Flies heterozygous for the deletion do not show a Minute bristle phenotype.

(Marygold et al., 2007)

Heterozygosity for Df(2L)Prl results in 1.5% X chromosome nondisjunction and 1.5% fourth chromosome nondisjunction in In(1)FM7/X ; svspa-pol females.

(Harris et al., 2003)

Df(2L)Prl in combination with a pair of introgressions from D.simulans spanning 30F1-31E7 to 35D7-36A14 Dsim\Int(2L)S and 21A1 to 22D1--23A2 Dsim\Int(2L)D produces sterile male flies. These flies are female sterile.

(Sawamura et al., 2000)

Shows dominant enhancement of dominant haltere phenotype caused by Ubx195 and Ubx9.22.

(Burnette et al., 1999)

Does not cause unconditional lethality in hybrid females when heterozygous with D.simulans chromosome.

(Coyne et al., 1998)

No second site non-complementing phenotype with zipEbr and zipmhc-c6.1.

(Halsell and Kiehart, 1998)

Shows no maternal enhancement of dpphr4.

(Nicholls and Gelbart, 1998)

Dominantly enhances the rough eye phenotype of Df(1)Δ59 hemizygotes.

(Bahri et al., 1997)

Mutants exhibit slight reduction in wing blade size and lack of wing vein L5.

(Cifuentes and Garcia-Bellido, 1997)

Dominantly causes tergite defects in less than 50% of run3 heterozygotes.

(Duffy et al., 1996)

Mutant cuticular phenotype of prd4/Df(2L)Prl transheterozygous embryos is rescued partially by one copy of and completely by two copies of P{prd-Gsb} and P{prd-Pax3}, two copies of P{prd-Gsb} can rescue embryos to adult flies.

(Xue and Noll, 1996)

Approximately 70% of homozygotes lack cells from the RP2/sib lineage, generally in only one or two hemisegments. All Df(2L)prd1.7/Df(2L)Prl embryos have hemisegments which show RP2/sib lineage defects.

(Bhat et al., 1995)

Deficient embryos show a variably penetrant mutant midgut phenotype: constrictions absent.

(Bilder and Scott, 1995)

Homozygous embryos do not complete head involution and tracheae and salivary glands are variable in size. The midgut does not constrict and the hindgut is slightly reduced in length. The Malpighian tubules are variably reduced in length.

(Harbecke and Lengyel, 1995)

Heterozygosity for this deletion has no effect on the mutant ovarian phenotype of ovoD2.

(Pauli et al., 1995)

RP2 lineage is normal in deficiency embryos.

(Bhat and Schedl, 1994)

prd4/Df(2L)Prl transheterozygotes only survive to adulthood in the presence of a copy of P{prd-SN20}. These adults give rise to fertile offspring.

(Gutjahr et al., 1994)
Stocks (3)
Notes on Origin
Discoverer

B.S. Baker.

(Carpenter, 1993.8.8)
 

The stock contains a second-site deletion uncovering l(2)gl.

(Roegiers et al., 2009)

In addition to the medial 2L deletion, the Df(2L)Prl chromosome studied is deleted for a number of genes at the tip of 2L, most likely due to a terminal deletion with its breakpoint in Ir21a or CG31973 or in the region between them - the breakpoint lies in the range 2L:22535..28754 (R5) (predicted cytology: 21B1). It is not known if all Df(2L)Prl chromosomes carry this terminal deletion.

(Yamamoto et al., 2009.2.25)
Balancer / Genotype Variants of the Aberration
 
Separable Components
 
Other Comments
 

The Df(2L)Prl chromosome contains a lesion in the 21A region, in addition to the deficiency at 32F1-33F2.

(Brumby et al., 2004)

The Df(2L)Prl chromosome may act as a dominant suppressor of telomeric silencing (assayed using the effect of the chromosome on the eye colour phenotype of flies carrying "P{wvar}KR3-2", a stable "brown-red" variant of the P{3'WP-2,wvar}2Lt insertion), but the eye colour phenotype in the presence of Df(2L)Prl overlaps the eye colour phenotype in a wild-type background so it cannot be unequivocally demonstrated that the deficiency chromosome uncovers a suppressor of telomeric silencing. In addition, the deficiency chromosome fails to complement lethal mutations of l(2)gl.

(Mason et al., 2004)
Synonyms and Secondary IDs (9)
References (83)