FB2024_03 , released June 25, 2024
Gene: Dmel\dgt2
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General Information
Symbol
Dmel\dgt2
Species
D. melanogaster
Name
dim γ-tubulin 2
Annotation Symbol
CG16969
Feature Type
protein_coding_gene
FlyBase ID
FBgn0032390
Gene Model Status
Current
Stock Availability
6 publicly available
Gene Summary
As part of the augmin complex, plays a role in centrosome-independent generation of spindle microtubules (PubMed:18443220). The complex is required for mitotic spindle assembly through its involvement in localizing gamma-tubulin to spindle microtubules (PubMed:17412918). dgt2 binds to microtubules in vitro (PubMed:19289792). (UniProt, Q9VKD6)
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All Summaries
Key Links
Genomic Location
Cytogenetic map
33B5-33B5
Sequence location
Recombination map
2-45
RefSeq locus
NT_033779 REGION:11989936..11990979
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (8 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables microtubule binding
inferred from direct assay
(Meireles et al., 2009)
enables protein binding
inferred from physical interaction with FLYBASE:wac; FB:FBgn0035120
(Meireles et al., 2009)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in mitotic spindle-templated microtubule nucleation
inferred from direct assay
(Tariq et al., 2020)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in mitotic spindle assembly
inferred by curator from GO:0070652
(Chen et al., 2017)
involved_in mitotic spindle-templated microtubule nucleation
inferred by curator from GO:0070652
(Chen et al., 2017)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
part_of HAUS complex
inferred from direct assay
(Tariq et al., 2020, Chen et al., 2017)
located_in meiotic spindle
inferred from direct assay
(Colombié et al., 2013)
part_of protein-containing complex
inferred from direct assay
(Meireles et al., 2009)
inferred from genetic interaction with FLYBASE:wac; FB:FBgn0035120
(Meireles et al., 2009)
located_in spindle
inferred from direct assay
(Meireles et al., 2009, Goshima et al., 2007)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
    -
    Summaries
    Gene Group (FlyBase)
    AUGMIN COMPLEX -
    The augmin complex (known as homologous to augmin subunits (HAUS) complex in vertebrates) is an eight-subunit protein complex involved in the mitotic spindle assembly. The augmin complex acts as an adaptor, recruiting the gamma-tubulin ring complex (γ-TuRC) to existing spindle microtubules to nucleate branching to increase spindle density. (Adapted from PMID:36114186 and FBrf0235489.)
    Protein Function (UniProtKB)
    As part of the augmin complex, plays a role in centrosome-independent generation of spindle microtubules (PubMed:18443220). The complex is required for mitotic spindle assembly through its involvement in localizing gamma-tubulin to spindle microtubules (PubMed:17412918). dgt2 binds to microtubules in vitro (PubMed:19289792).
    (UniProt, Q9VKD6)
    Gene Model and Products
    Number of Transcripts
    2
    Number of Unique Polypeptides
    1

    Please see the JBrowse view of Dmel\dgt2 for information on other features

    To submit a correction to a gene model please use the Contact FlyBase form

    Protein Domains (via Pfam)
    Isoform displayed:
    Pfam protein domains
    InterPro name
    classification
    start
    end
    Protein Domains (via SMART)
    Isoform displayed:
    SMART protein domains
    InterPro name
    classification
    start
    end
    Structure
    Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VKD6)

    If you don't see a structure in the viewer, refresh your browser.
    Model Confidence:
    • Very high (pLDDT > 90)
    • Confident (90 > pLDDT > 70)
    • Low (70 > pLDDT > 50)
    • Very low (pLDDT < 50)

    AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

    Experimentally Determined Structures
    Crossreferences
    Comments on Gene Model

    Gene model reviewed during 5.51

    Sequence Ontology: Class of Gene
    nuclear_gene
    protein_coding_gene
    Transcript Data
    Annotated Transcripts
    Name
    FlyBase ID
    RefSeq ID
    Length (nt)
    Assoc. CDS (aa)
    dgt2-RA
    FBtr0080285
    NM_135687
    842
    231
    dgt2-RB
    FBtr0342580
    NM_001298927
    987
    231
    Additional Transcript Data and Comments
    Reported size (kB)
    Comments
    External Data
    Crossreferences
    Polypeptide Data
    Annotated Polypeptides
    Name
    FlyBase ID
    Predicted MW (kDa)
    Length (aa)
    Theoretical pI
    UniProt
    RefSeq ID
    GenBank
    dgt2-PA
    FBpp0079869
    25.8
    231
    6.27
    Q9VKD6
    NP_609531
    AAF53140
    dgt2-PB
    FBpp0309535
    25.8
    231
    6.27
    Q9VKD6
    NP_001285856
    AHN54370
    Polypeptides with Identical Sequences

    The group(s) of polypeptides indicated below share identical sequence to each other.

    231 aa isoforms: dgt2-PA, dgt2-PB
    Additional Polypeptide Data and Comments
    Reported size (kDa)
    Comments
    External Data
    Subunit Structure (UniProtKB)

    Component of the augmin complex composed of dgt2, dgt3, dgt4, dgt5, dgt6, msd1, msd5 and wac (PubMed:18443220, PubMed:19289792). The complex interacts directly or indirectly with microtubules and is required for centrosome-independent generation of spindle microtubules (PubMed:18443220). dgt2 interacts directly with wac (via coiled coil) (PubMed:19289792).

    (UniProt, Q9VKD6)
    Crossreferences
    Linkouts
    Sequences Consistent with the Gene Model
    Mapped Features

    Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\dgt2 using the Feature Mapper tool.

    External Data
    Crossreferences
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Linkouts
    Expression Data
    Testis-specificity index

    The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

    2.27

    Transcript Expression
    in situ
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    embryonic stage 1 -- 3
    organism

    Comment: maternally deposited

    (Fisher et al., 2012)
    embryonic stage 7 -- 8
    organism | ubiquitous
    (Fisher et al., 2012)
    embryonic stage 9 -- 10
    organism | ubiquitous
    (Fisher et al., 2012)
    embryonic stage 11 -- 12
    organism | ubiquitous
    (Fisher et al., 2012)
    Additional Descriptive Data
    Marker for
     
    Subcellular Localization
    CV Term
    Polypeptide Expression
    Additional Descriptive Data
    Marker for
     
    Subcellular Localization
    CV Term
    Evidence
    References
    part_of HAUS complex
    inferred from direct assay
    (Tariq et al., 2020, Chen et al., 2017)
    located_in meiotic spindle
    inferred from direct assay
    (Colombié et al., 2013)
    part_of protein-containing complex
    inferred from direct assay
    (Meireles et al., 2009)
    inferred from genetic interaction with FLYBASE:wac; FB:FBgn0035120
    (Meireles et al., 2009)
    located_in spindle
    inferred from direct assay
    (Meireles et al., 2009, Goshima et al., 2007)
    Expression Deduced from Reporters
    High-Throughput Expression Data
    Associated Tools

    JBrowse - Visual display of RNA-Seq signals

    View Dmel\dgt2 in JBrowse
    RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
    Bulk Downloads
    RNA-Seq RPKM values for all genes
    Reference
    See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
    Developmental Proteome: Life Cycle
    Developmental Proteome: Embryogenesis
    External Data and Images
    Linkouts
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
    EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    Images
    FlyExpress - Embryonic expression images (BDGP data)
    • Stages(s) 1-3
    • Stages(s) 7-8
    • Stages(s) 9-10
    • Stages(s) 11-12
    FlyBase Wiki
    Image Based Resources
    Alleles, Insertions, Transgenic Constructs, and Aberrations
    Classical and Insertion Alleles ( 3 )
    For All Classical and Insertion Alleles Show
     
    Other relevant insertions
    Transgenic Constructs ( 6 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of dgt2
    Transgenic constructs containing regulatory region of dgt2
    Aberrations (Deficiencies and Duplications) ( 1 )
    Inferred from experimentation ( 1 )
    Gene disrupted in
    Df(2L)Exel6031
    (Ryder, 2004.4.26)
    Inferred from location ( 0 )
    Variants
    Variant Molecular Consequences
    Alleles Representing Disease-Implicated Variants
    Phenotypes
    For more details about a specific phenotype click on the relevant allele symbol.
    Phenotype manifest in
    Allele
    Orthologs
    Downloads
    Download All DIOPT Orthologs
    Human Orthologs (via DIOPT v9.1)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Source
    Compara
    Domainoid
    eggNOG
    Hieranoid
    Homologene
    Inparanoid
    OMA
    OrthoDB
    OrthoFinder
    OrthoInspector
    orthoMCL
    Panther
    Phylome
    SonicParanoid
    Alignment
    Complementation?
    Transgene?
    Homo sapiens (Human) (0)
    Model Organism Orthologs (via DIOPT v9.1)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Source
    Compara
    Domainoid
    eggNOG
    Hieranoid
    Homologene
    Inparanoid
    OMA
    OrthoDB
    OrthoFinder
    OrthoInspector
    orthoMCL
    Panther
    Phylome
    SonicParanoid
    Alignment
    Complementation?
    Transgene?
    Rattus norvegicus (Norway rat) (0)
    Mus musculus (laboratory mouse) (0)
    Xenopus tropicalis (Western clawed frog) (0)
    Danio rerio (Zebrafish) (0)
    Caenorhabditis elegans (Nematode, roundworm) (0)
    Anopheles gambiae (African malaria mosquito) (1)
    Agam\AgaP_AGAP003225
    7 of 12
    Yes
    Yes
    Arabidopsis thaliana (thale-cress) (0)
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    Schizosaccharomyces pombe (Fission yeast) (0)
    Escherichia coli (enterobacterium) (0)
    Other Organism Orthologs (via OrthoDB)
    Data provided directly from OrthoDB:dgt2. Refer to their site for version information.
    Paralogs
    Downloads
    Download All DIOPT Paralogs
    Paralogs (via DIOPT v9.1)
    Human Disease Associations
    FlyBase Human Disease Model Reports
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Evidence
      References
      Potential Models Based on Orthology ( 0 )
      Human Ortholog
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Interaction
      References
      Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      DO term
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Show neighbor-neighbor interactions:
      Show/hide secondary interactors 
      (data from AllianceMine provided by esyN)
      Select Layout:
      Legend:
      Protein
      RNA
      Selected Interactor(s)
      Other Interaction Browsers
      View in FlyBase Interactions Browser   View in MIST tool (external link)
      Please see the Physical Interaction reports below for full details
      protein-protein
      Physical Interaction
      Assay
      References
      dgt2 - dgt3
      experimental knowledge based, anti tag coimmunoprecipitation, western blot, cosedimentation, molecular sieving
      (Guruharsha et al., 2011, Goshima et al., 2008)
      dgt2 - dgt4
      experimental knowledge based, anti tag coimmunoprecipitation, western blot, cosedimentation, molecular sieving
      (Guruharsha et al., 2011, Goshima et al., 2008)
      dgt2 - dgt5
      experimental knowledge based, molecular sieving, anti tag coimmunoprecipitation, western blot, cosedimentation
      (Guruharsha et al., 2011, Goshima et al., 2008)
      dgt2 - dgt6
      molecular sieving, cosedimentation, experimental knowledge based, anti tag coimmunoprecipitation, western blot
      (Guruharsha et al., 2011, Goshima et al., 2008)
      dgt2 - msd1
      anti tag coimmunoprecipitation, cross-linking study, Identification by mass spectrometry, molecular weight estimation by staining, experimental knowledge based
      (Tariq et al., 2020, Chen et al., 2017, Guruharsha et al., 2011)
      dgt2 - msd5
      experimental knowledge based
      (Guruharsha et al., 2011)
      dgt2 - wac
      pull down, autoradiography, experimental knowledge based, anti bait coimmunoprecipitation, western blot
      (Guruharsha et al., 2011, Meireles et al., 2009)
      Summary of Genetic Interactions
      esyN Network Diagram
      Show/hide secondary interactors 
      (data from AllianceMine provided by esyN)
      esyN Network Key:
      Suppression
      Enhancement
      Other Interaction Browsers
      View in FlyBase Interactions Browser   View in MIST tool (external link)
      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Ras85D
      enhanceable
      dgt2
      (Friedman et al., 2011)
      External Data
      Subunit Structure (UniProtKB)
      Component of the augmin complex composed of dgt2, dgt3, dgt4, dgt5, dgt6, msd1, msd5 and wac (PubMed:18443220, PubMed:19289792). The complex interacts directly or indirectly with microtubules and is required for centrosome-independent generation of spindle microtubules (PubMed:18443220). dgt2 interacts directly with wac (via coiled coil) (PubMed:19289792).
      (UniProt, Q9VKD6 )
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      External Data
      Linkouts
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      2L
      Recombination map
      2-45
      Cytogenetic map
      33B5-33B5
      Sequence location
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      33B5-33B5
      Limits computationally determined from genome sequence between P{EP}MESK1EP980 and P{PZ}l(2)0181001810&P{EP}CG6785EP340
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Experimentally Determined Recombination Data
      Location

      2-45

      (FlyBase, 2016)

      2-39.0

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (6)
      Genomic Clones (14)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (34)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        Antibody Information
        Laboratory Generated Antibodies
         

        polyclonal

        (Meireles et al., 2009, Goshima et al., 2008)
        Commercially Available Antibodies
         
        Cell Line Information
        Publicly Available Cell Lines
         
          Other Stable Cell Lines
           
            Other Comments

            RNAi screen using dsRNA made from templates generated with primers directed against this gene results in decreased γ-tubulin staining of the centrosome and spindle and the formation of an aberrantly long spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

            (Goshima et al., 2007)
            Relationship to Other Genes
            Source for database merge of
            Additional comments

            FlyBase curator comment: the insertion in the "d01514" Exelixis line (P{XP}escld01514) was originally assigned to the CG16969 gene in FBrf0179797, resulting in the "CG16969d01514" (FBal0162083) allele. However, FBrf0184340 shows that the insertion is actually within escl.

            (Bloomington Drosophila Stock Center, 2004.11.6)
            Nomenclature History
            Source for database identify of
            Nomenclature comments
            Etymology

            Named "dim γ-tubulin 2" for the decreased γ-tubulin staining of the spindle pole following treatment of S2 cells with dgt2.

            (Goshima et al., 2007)
            Synonyms and Secondary IDs (4)
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 30 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            FlyMine - An integrated database for Drosophila genomics
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            MIST (protein-protein) - An integrated Molecular Interaction Database
            References (38)