FB2024_03 , released June 25, 2024
Recombinant Construct: P{srp.Hemo-GAL4}
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General Information
Symbol
P{srp.Hemo-GAL4}
FlyBase ID
FBtp0023390
Feature type
transgenic_transposable_element
Component Allele(s)
Scer\GAL4srp.Hemo
Also Known As
srp-Gal4, srpHemo-Gal4, srpHemoGAL4, srp(hemo)Gal4, srp.Hemo-GAL4, serpentHemoGAL4, serpent-GAL4, serpentGal4
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Scer\GAL4srp.Hemo
Transgenic product class
Component
Use(s)
Regulatory region(s)
srp
Encoded product / tool
GAL4
Description

Expression of a GAL4 driver is under the control of 2 genomic sequences from upstream of srp amplified with AGGGTACCCTACTGCTTCCCACTCTAAGACTTCCAGTTTTAGGCTACG (sense) and GGAATTCGGCAATGCCCCACCCCTTGGCTGGACGG (antisense) as well as CGCGGTACCCAGCGGGAGCAACAGGATCAAATGCAGCAGCG (sense) and CGCGGTACCTATGGGATCCGTGCTGGGGTAGTGCTCGTAGAGC (antisense).

(Bruckner et al., 2004)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
embryonic/larval hemocyte
(Kuraishi et al., 2009)
embryonic stage 9
embryonic hemocyte anlage
(Bruckner et al., 2004)
embryonic stage 9 -- 17
procrystal cell
(Bruckner et al., 2004)
embryonic stage 10 -- 15
embryonic/larval hemocyte
(Bruckner et al., 2004)
embryonic/larval head mesoderm derived hemocyte
(Bruckner et al., 2004)
embryonic stage 10 -- 17
embryonic/larval plasmatocyte
(Bruckner et al., 2004)
embryonic/larval crystal cell
(Bruckner et al., 2004)
embryonic stage 12 -- 15
embryonic/larval plasmatocyte
(Weavers et al., 2016)
embryonic stage 12 -- 17
embryonic/larval hemocyte
(Martinek et al., 2008)
embryonic stage 14 -- 17
embryonic/larval hemocyte
(Wood et al., 2006)
embryonic stage 15 -- 17
embryonic/larval hemocyte
(Bruckner et al., 2004)
embryonic stage 16 -- 17
embryonic/larval hemocyte
(Bunt et al., 2011)
larval stage
embryonic/larval pericardial cell
(Pendse et al., 2013)
embryonic/larval garland cell
(Pendse et al., 2013)
embryonic/larval hemocyte
(Pendse et al., 2013, Matova and Anderson, 2006)
embryonic/larval lymph gland
(Matova and Anderson, 2006)
first instar larval stage -- third instar larval stage
embryonic/larval pericardial cell

Comment: from 48 hr AEL

(Das et al., 2008)
Additional Information
Statement
Reference

Drives expression in hemocytes and pericardial cells (nephrocytes) from embryonic stage 9 through all larval stages.

(FlyBase Curators, 2017)

ScerGAL4srp.Hemo drives expression in nephrocytes and hemocytes

(Pendse et al., 2013)

ScerGAL4srp.Hemo drives expression in all hemocytes and macrophages at all embryonic stages, as well as in the head mesoderm that gives rise to hemocytes beginning at embryonic stage 9.

(Bruckner et al., 2004)
 
Marker for
embryonic/larval hemocyte
(Bunt et al., 2011, Martinek et al., 2008)
embryonic/larval pericardial cell
(Das et al., 2008)
hemocyte
(Siekhaus et al., 2010, Kuraishi et al., 2009)
Reflects expression of
srp
(Weavers et al., 2016, Pendse et al., 2013, Bunt et al., 2011, Kuraishi et al., 2009, Das et al., 2008, Martinek et al., 2008, Wood et al., 2006, Bruckner et al., 2004)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
78565
y1 w*; P{srp.Hemo-GAL4}2, P{UAS-GFP.U}3
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (26)
Reported As
Symbol Synonym
(srphemo)-Gal4
(Liu et al., 2015)
P{srp.Hemo-GAL4}
(FlyBase, 1992-)
P{srp.Hemo-Gal4}
(Verboon et al., 2015)
Serpent-Gal4
(Kalamarz et al., 2012)
Serpent-hemocytes-Gal4
(Parisi et al., 2013)
SerpentHemo-Gal4
(Paddibhatla et al., 2010)
Srp-Gal4
(Gueguen et al., 2013, Akbar et al., 2011, Ninov et al., 2007)
SrpHemo-GAL4
(Martinek et al., 2008)
SrpHemo-Gal4
(Kalamarz et al., 2012)
serpent-GAL4
(Das et al., 2008)
serpentGal4
(Hakim-Mishnaevski et al., 2019, Hilu-Dadia et al., 2018)
serpentHemoGAL4
(Wood et al., 2006)
serpentHemoGal4
(Campbell et al., 2021)
srp hemo-GAL4
(Krieser et al., 2007)
srp(hemo)Gal4
(Bazzi et al., 2023, Pavlidaki et al., 2022, Cattenoz et al., 2020, Trébuchet et al., 2019)
srp-GAL4
(Coates et al., 2021, Kuraishi et al., 2009)
srp-Gal4
(Lehne and Bogdan, 2023, Campbell et al., 2021, Valoskova et al., 2019, Matsubayashi et al., 2017, Guillou et al., 2016, Weavers et al., 2016, Bausek and Zeidler, 2014, Rahman et al., 2012, Bunt et al., 2011, Love et al., 2011, Stramer et al., 2010, Zanet et al., 2009)
srp-gal4
(Stramer et al., 2008)
srp.Hemo-GAL4
(Verboon et al., 2015, Pendse et al., 2013)
srp.Hemo-Gal4
(Tsuyama et al., 2023, Verboon et al., 2015)
srpG4
(Hakim-Mishnaevski et al., 2019)
srpGal4
(Shlyakhover et al., 2018)
srpHemo-GAL
(Das et al., 2008)
srpHemo-GAL4
(Giordani et al., 2023, Emtenani et al., 2022, Coates et al., 2021, Sanchez Bosch et al., 2019, Valoskova et al., 2019, Makhijani et al., 2011, Nagaosa et al., 2011, Kuraishi et al., 2009)
srpHemo-Gal4
(Belyaeva et al., 2022, Matsubayashi et al., 2020, Fuwa et al., 2015, Bunt et al., 2010, Matova and Anderson, 2006)
srpHemoGAL4
(Matsubayashi et al., 2017, Nonaka et al., 2013, Tung et al., 2013, Fujita et al., 2012, Siekhaus et al., 2010, Wood et al., 2006, Bruckner et al., 2004, Bruckner et al., 2004)
Secondary FlyBase IDs
    References (54)