FB2024_03 , released June 25, 2024
Recombinant Construct: P{SUPor-P.PRE#2}
Open Close
General Information
Symbol
P{SUPor-P.PRE#2}
FlyBase ID
FBtp0009269
Feature type
transgenic_transposable_element
Component Allele(s)
gypsy\su(Hw)BR430
UbxPRE1.6
wBR.E.BR
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele wBR.E.BR
Transgenic product class
Component
Use(s)
Encoded product / tool
w
Description

P{SUPor-P-lt} contains a mini-w gene that has two flanking gypsy\su(Hw)BR sites, one of which is upstream of the w eye promoter.

(Bhadra et al., 1999)

Construct: w+mC minigene altered to include a su(Hw) binding region (all 12 binding sites, nucleotides 647 to 1077 of Marlor et al., Mol Cell Biol 6:1129-1134 ), upstream of the w eye enhancer (nucleotides -1084 to -1465 of Qian et al., Genetics 131:79-90 ), upstream of the w coding region, upstream of a second su(Hw) binding region.

(Roseman et al., 1993)

Two gypsy\su(Hw)BR binding sites flank the w+mET sequences to insulate the w gene from chromosomal position effects.

(Roseman et al., 1995)
Component allele UbxPRE1.6
Transgenic product class
Component
Use(s)
Encoded product / tool
UbxPRE
Description

Construct: 1.6kb StyI-EcoRI fragment containing the Ubx PRE (Polycomb group response element).

(Mallin et al., 1998)
Component allele gypsy\su(Hw)BR430
Transgenic product class
Component
Use(s)
Encoded product / tool
gypsy\su(Hw)BR
Description

Contains 12 su(Hw)-binding sites, corresponding to bp 647-1077 in the gypsy transposon.

(Kuhn et al., 2003)

Construct: Consists of a 430bp gypsy\su(Hw)BR, including all 12 binding sites.

(Patton et al., 1992)

Consists of a 430bp gypsy\su(Hw)BR, including all 12 binding sites, nucleotides 647 to 1077 of FBrf0045014.

(Roseman et al., 1993)

Consists of a 430bp gypsy\su(Hw)BR, gypsy sequences 647-1077 (coordinates of FBrf0045014).

(Roseman et al., 1995)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments

Constructs P{SUPor-P.PRE#2} and P{SUPor-P.PRE#6} differ in the orientation of the fragment containing the y enhancer, y coding region and UbxPRE. In construct P{SUPor-P.PRE#6} the y gene is transcribed in the opposite orientation to the w gene present in P{SUPor-P.PRE#6}. In construct P{SUPor-P.PRE#2}, the fragment containing the y enhancer, y coding region and UbxPRE has been reversed so that the y gene is transcribed in the same orientation as the w gene.

(Mallin et al., 1998)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{SUPor-P.PRE#2}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)