Constructs P{SUPor-P.PRE#2} and P{SUPor-P.PRE#6} differ in the orientation of the fragment containing the y enhancer, y coding region and UbxPRE. In construct P{SUPor-P.PRE#6} the y gene is transcribed in the opposite orientation to the w gene present in P{SUPor-P.PRE#6}. In construct P{SUPor-P.PRE#2}, the fragment containing the y enhancer, y coding region and UbxPRE has been reversed so that the y gene is transcribed in the same orientation as the w gene.
Constructs P{SUPor-P.PRE#2} and P{SUPor-P.PRE#6} differ in the orientation of the fragment containing the y enhancer, y coding region and UbxPRE. In construct P{SUPor-P.PRE#6} the y gene is transcribed in the opposite orientation to the w gene present in P{SUPor-P.PRE#6}. In construct P{SUPor-P.PRE#2}, the fragment containing the y enhancer, y coding region and UbxPRE has been reversed so that the y gene is transcribed in the same orientation as the w gene.