Transgenic construct used to characterize a silencer from the Ubx gene. Contains a reporter in which the 0.6kb Ubx embryonic PBX enhancer is fused upstream of the 2.8kb Ubx imaginal disc enhancer IDE which is linked to the proximal Ubx promoter followed by sequence encoding lacZ. A 1.56kb Ubx fragment containing a Polycomb group response element (PRE) has been inserted in between the PBX and IDE enhancers.
Construct: 1.6kb StyI-EcoRI fragment containing the Ubx PRE (Polycomb group response element).