FlyBase Allele Report: Dmel\w[BR.E.BR]

General Information

Symbol

Dmel\w^BR.E.BR

Species

D. melanogaster

Name

FlyBase ID

FBal0033130

Feature type

allele

Associated gene

Dmel\w

Associated Insertion(s)

Carried in Construct

P{SUPor-P.PRE#2}

P{SUPor-P.PRE#6}

P{SUPor-P}

Also Known As

P{SUPor-P}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Roseman et al., 1993)

Progenitor genotype

w^+mET

(Roseman et al., 1993)

Carried in construct

P{SUPor-P.PRE#2}

P{SUPor-P.PRE#6}

P{SUPor-P}

Cytology

Description

P{SUPor-P-lt} contains a mini-w gene that has two flanking gypsy\su(Hw)BR sites, one of which is upstream of the w eye promoter.

(Bhadra et al., 1999)

Two gypsy\su(Hw)BR binding sites flank the w^+mET sequences to insulate the w gene from chromosomal position effects.

(Roseman et al., 1995)

Construct: w^+mC minigene altered to include a su(Hw) binding region (all 12 binding sites, nucleotides 647 to 1077 of Marlor et al., Mol Cell Biol 6:1129-1134 ), upstream of the w eye enhancer (nucleotides -1084 to -1465 of Qian et al., Genetics 131:79-90 ), upstream of the w coding region, upstream of a second su(Hw) binding region.

(Roseman et al., 1993)

Allele components

Component

Use(s)

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eye color

(Roseman et al., 2001, Kurenova et al., 1998)

visible | recessive

(Roseman et al., 1993)

Phenotype Manifest In

pigment cell

(Roseman et al., 2001, Kurenova et al., 1998, Roseman et al., 1993)

Detailed Description

Statement

Reference

Eye color: red

(Roseman et al., 2001)

Eye colour: flies carrying w^BR.E.BR have red eyes.

(Soldatov et al., 1999)

Eye colour: Orange to dark orange (depending on insertion site).

(Kurenova et al., 1998)

Eye colour: highly pigmented, in the majority of flies.

(Roseman et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has abnormal eye color phenotype, enhanceable by Pc^{ZnF.hs.Tag:FLAG}

(Roseman et al., 2001)

su(Hw)^V/su(Hw)^E8, w^BR.E.BR has abnormal eye color phenotype, enhanceable by Pc^{ZnF.hs.Tag:FLAG}

(Roseman et al., 2001)

su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has abnormal eye color phenotype, enhanceable by esc^{ZnF.hs.Tag:FLAG}

(Roseman et al., 2001)

su(Hw)^V/su(Hw)^E8, w^BR.E.BR has abnormal eye color phenotype, enhanceable by esc^{ZnF.hs.Tag:FLAG}

(Roseman et al., 2001)

su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has abnormal eye color phenotype, enhanceable by Scm^{ZnF.hs.Tag:FLAG}

(Roseman et al., 2001)

su(Hw)^V/su(Hw)^E8, w^BR.E.BR has abnormal eye color phenotype, enhanceable by Scm^{ZnF.hs.Tag:FLAG}

(Roseman et al., 2001)

w^BR.E.BR, z^v77h has abnormal eye color phenotype, enhanceable by Taf9¹

(Soldatov et al., 1999)

su(Hw)^V/su(Hw)², w^BR.E.BR, z^v77h has abnormal eye color phenotype, enhanceable by Taf9¹

(Soldatov et al., 1999)

Taf9¹, w^BR.E.BR, z^v77h has abnormal eye color phenotype, enhanceable by su(Hw)^V/su(Hw)²

(Soldatov et al., 1999)

NOT Enhanced by

Statement

Reference

su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has abnormal eye color phenotype, non-enhanceable by esc^{ZnF.hs.Tag:FLAG}

(Roseman et al., 2001)

su(Hw)^V/su(Hw)^E8, w^BR.E.BR has abnormal eye color phenotype, non-enhanceable by esc^{ZnF.hs.Tag:FLAG}

(Roseman et al., 2001)

su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has abnormal eye color phenotype, non-enhanceable by Scm^{ZnF-ΔSPM.hs.Tag:FLAG}

(Roseman et al., 2001)

su(Hw)^V/su(Hw)^E8, w^BR.E.BR has abnormal eye color phenotype, non-enhanceable by Scm^{ZnF-ΔSPM.hs.Tag:FLAG}

(Roseman et al., 2001)

Suppressed by

Statement

Reference

Scm^{ZnF.hs.Tag:FLAG}, su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has abnormal eye color phenotype, suppressible by ph-p⁴⁰⁹

(Roseman et al., 2001)

Scm^{ZnF.hs.Tag:FLAG}, su(Hw)^V/su(Hw)^E8, w^BR.E.BR has abnormal eye color phenotype, suppressible by ph-p⁴⁰⁹

(Roseman et al., 2001)

Pc^{ZnF.hs.Tag:FLAG}, su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has abnormal eye color phenotype, suppressible by ph-p⁴⁰⁹

(Roseman et al., 2001)

Pc^{ZnF.hs.Tag:FLAG}, su(Hw)^V/su(Hw)^E8, w^BR.E.BR has abnormal eye color phenotype, suppressible by ph-p⁴⁰⁹

(Roseman et al., 2001)

esc^{ZnF.hs.Tag:FLAG}, su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has abnormal eye color phenotype, suppressible by ph-p⁴⁰⁹

(Roseman et al., 2001)

esc^{ZnF.hs.Tag:FLAG}, su(Hw)^V/su(Hw)^E8, w^BR.E.BR has abnormal eye color phenotype, suppressible by ph-p⁴⁰⁹

(Roseman et al., 2001)

Other

Statement

Reference

w^BR.E.BR, z^v77h has abnormal eye color phenotype

(Soldatov et al., 1999)

su(Hw)^V/su(Hw)², w^BR.E.BR, z^v77h has abnormal eye color phenotype

(Soldatov et al., 1999)

Phenotype Manifest In

Enhanced by

Statement

Reference

su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, enhanceable by Pc^{ZnF.hs.Tag:FLAG}

(Roseman et al., 2001)

su(Hw)^V/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, enhanceable by Pc^{ZnF.hs.Tag:FLAG}

(Roseman et al., 2001)

su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, enhanceable by esc^{ZnF.hs.Tag:FLAG}

(Roseman et al., 2001)

su(Hw)^V/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, enhanceable by esc^{ZnF.hs.Tag:FLAG}

(Roseman et al., 2001)

su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, enhanceable by Scm^{ZnF.hs.Tag:FLAG}

(Roseman et al., 2001)

su(Hw)^V/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, enhanceable by Scm^{ZnF.hs.Tag:FLAG}

(Roseman et al., 2001)

w^BR.E.BR, z^v77h has pigment cell phenotype, enhanceable by Taf9¹

(Soldatov et al., 1999)

su(Hw)^V/su(Hw)², w^BR.E.BR, z^v77h has pigment cell phenotype, enhanceable by Taf9¹

(Soldatov et al., 1999)

Taf9¹, w^BR.E.BR, z^v77h has pigment cell phenotype, enhanceable by su(Hw)^V/su(Hw)²

(Soldatov et al., 1999)

NOT Enhanced by

Statement

Reference

su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, non-enhanceable by esc^{ZnF.hs.Tag:FLAG}

(Roseman et al., 2001)

su(Hw)^V/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, non-enhanceable by esc^{ZnF.hs.Tag:FLAG}

(Roseman et al., 2001)

su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, non-enhanceable by Scm^{ZnF-ΔSPM.hs.Tag:FLAG}

(Roseman et al., 2001)

su(Hw)^V/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, non-enhanceable by Scm^{ZnF-ΔSPM.hs.Tag:FLAG}

(Roseman et al., 2001)

Suppressed by

Statement

Reference

esc^{ZnF.hs.Tag:FLAG}, su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, suppressible by ph-p⁴⁰⁹

(Roseman et al., 2001)

esc^{ZnF.hs.Tag:FLAG}, su(Hw)^V/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, suppressible by ph-p⁴⁰⁹

(Roseman et al., 2001)

Scm^{ZnF.hs.Tag:FLAG}, su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, suppressible by ph-p⁴⁰⁹

(Roseman et al., 2001)

Scm^{ZnF.hs.Tag:FLAG}, su(Hw)^V/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, suppressible by ph-p⁴⁰⁹

(Roseman et al., 2001)

Pc^{ZnF.hs.Tag:FLAG}, su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, suppressible by ph-p⁴⁰⁹

(Roseman et al., 2001)

Pc^{ZnF.hs.Tag:FLAG}, su(Hw)^V/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, suppressible by ph-p⁴⁰⁹

(Roseman et al., 2001)

Other

Statement

Reference

w^BR.E.BR, z^v77h has pigment cell phenotype

(Soldatov et al., 1999)

su(Hw)^V/su(Hw)², w^BR.E.BR, z^v77h has pigment cell phenotype

(Soldatov et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of w^BR.E.BR is reduced (in a background of reduced su(Hw)⁺) by the expression of tethered Pc, Scm or esc. In the case of Pc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG}, the effect is seen without the use of heat shock. For Scm^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} and esc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} heat shock must be applied daily. In the case of esc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} the effect is dependent on the site of insertion of P{SUPor-P}. ph-p⁴⁰⁹ completely suppresses the strong silencing of w^BR.E.BR in P{SUPor-P}22F caused by tethered Pc, and substantially suppresses the strong silencing of w^BR.E.BR in P{SUPor-P}22F caused by tethered Scm or esc.

(Roseman et al., 2001)

The eye colour is not affected if w^BR.E.BR flies are also carrying e(y)1¹ and z^v77h. Flies that are also carrying e(y)1¹, z^v77h and su(Hw)²/su(Hw)^V have reduced eye pigmentation.

(Soldatov et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1,968)

13035

13173

13233

13240

13246

13524

13639

13675

13797

13834

13847

13894

More stocks available...

Notes on Origin

Discoverer

Comments

Three insertion lines carrying this allele show variegation for w, and behave as though the insertions are heterochromatic. In situ hybridization confirms that the insertion in one of these lines is in the second chromosome centric heterochromatin. In a su(Hw) mutant background the variegating phenotype of these insertions becomes more pronounced, and the su(Hw) mutation acts dominantly in this interaction.

(Roseman et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

w^BR.E.BR

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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