FlyBase Experimental Tool Report: RS(I-SceI)

FB2024_03 , released June 25, 2024

FB2024_03 , released June 25, 2024

Experimental tool: RS(I-SceI)

General Information

Symbol

RS(I-SceI)

FlyBase ID

FBto0000906

Name

I-SceI recognition site

Description

Description

The RS(I-SceI) entry in FlyBase represents the recognition site for the I-SceI DNA endonuclease. The recognition site is 18bp in length 5'-TAGGGATAACAGGGTAAT-3'). Cleavage of the RS(I-SceI) site by I-SceI DNA endonuclease results in a double-strand DNA break (PMID:2183191).

Uses

nuclease target site

External Crossreferences and Linkouts

Related experimental tools

Compatible tools (1)

Tool

Uses

Transgenic Constructs

Carrying the tool (14)

Transgenic construct(s)

Component allele

Reg. region

Encoded product / tool

Tagged with

Also carries

Stocks

P{Col4a1.TARG}

Col4a1^{TARG.DsRed(Unk)}

Col4a1

DsRed(Unk): red fluorescent protein

RS(I-SceI): nuclease target site

Avic\GFP^{DarkLime.3xP3.Scer\SceI.RS}

3xP3

EGFP: green fluorescent protein

RS(I-SceI): nuclease target site

P{fru.C-5'donor}

fru

FRT: recombinase target site
RS(I-CreI): nuclease target site
RS(I-SceI): nuclease target site

P{fru.Δtra-5'donor}

fru^{Δtra-5'donor}

fru

FRT: recombinase target site
RS(I-CreI): nuclease target site
RS(I-SceI): nuclease target site

P{fru.F-5'donor}

fru

FRT: recombinase target site
RS(I-CreI): nuclease target site
RS(I-SceI): nuclease target site

P{fru.M-5'donor}

fru

FRT: recombinase target site
RS(I-CreI): nuclease target site
RS(I-SceI): nuclease target site

P{fru.X-3'donor}

fru

FRT: recombinase target site
RS(I-CreI): nuclease target site
RS(I-SceI): nuclease target site

P{Lemon}

Avic\GFP^{Lemon.3xP3.Scer\SceI.RS}

3xP3

Avic\GFP

RS(I-SceI): nuclease target site

P{y.F.TARG}

y

RS(I-SceI): nuclease target site

P{y.F-R.TARG}

y

RS(I-SceI): nuclease target site

P{y.G.TARG}

y

RS(I-SceI): nuclease target site

P{y.J.TARG}

y

RS(I-SceI): nuclease target site

P{y.K.TARG}

y

RS(I-SceI): nuclease target site

P{y.N.TARG}

y

RS(I-SceI): nuclease target site

Insertions ( 59 )

Insertion

Encoded product / tool

Tagged with

Also carries

Stocks

Associated Alleles

RS(I-SceI): nuclease target site

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.Rab1-GAL4.ATG(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.Rab2-GAL4.ATG(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.Rab3-GAL4.ATG(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.Rab4-GAL4.ATG(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.Rab5-GAL4.ATG(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.Rab6-GAL4.ORF(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.Rab7-GAL4.ORF(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.Rab8-GAL4.ATG(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.Rab9-GAL4.ATG(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.Rab10-GAL4.ATG(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.Rab11-GAL4.ORF(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.Rab14-GAL4.ATG(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.Rab18-GAL4.ORF(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.Rab19-GAL4.ORF(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.Rab21-GAL4.ORF(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.Rab23-GAL4.ATG(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.Rab26-GAL4.ex1(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.Rab27-GAL4.ORF(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.Rab30-GAL4.ORF(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.Rab32-GAL4.ex1(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.Rab35-GAL4.ATG(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.Rab39-GAL4.ATG(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.Rab40-GAL4.ATG(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.RabX1-GAL4.ORF(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.RabX4-GAL4.ORF(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.RabX5-GAL4.ORF(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{I-SceI(FRT.RabX6-GAL4.ORF(loxP.3xP3-RFP))}VK00033

FRT: recombinase target site
RS(I-SceI): nuclease target site

M{DR-white.mu}ZH-51C

RS(I-SceI): nuclease target site

M{DR-white}ZH-51C

RS(I-SceI): nuclease target site

M{W+G+R+.TS51D2xSce}ZH-51D

RS(I-SceI): nuclease target site

M{W+G+R+.TS51D}ZH-51D

RS(I-CreI): nuclease target site
RS(I-SceI): nuclease target site

M{W+G+R+.TS58A2xSce}ZH-58A

RS(I-SceI): nuclease target site

Mi{256xlacO.RS(I-SceI)}CG43428^{MI00794-256xlacO.RS(I-SceI)}

lacO: specific binding tool
RS(I-SceI): nuclease target site

CG43428^{MI00794-256xlacO.RS(I-SceI)}

Mi{256xlacO.RS(I-SceI)}MI03233-256xlacO.RS(I-SceI)

lacO: specific binding tool
RS(I-SceI): nuclease target site

Mi{256xlacO.RS(I-SceI)}MI03854-256xlacO.RS(I-SceI)

lacO: specific binding tool
RS(I-SceI): nuclease target site

Mi{DR-white.3xP3-DsRed}CG17167^{MI02024-DR-white}

RS(I-SceI): nuclease target site

CG17167^{MI02024-DR-white}

Mi{DR-white.3xP3-DsRed}CG43428^{MI00794-DR-white}

RS(I-SceI): nuclease target site

CG43428^{MI00794-DR-white}

Mi{DR-white.3xP3-DsRed}MI00192-DR-white

RS(I-SceI): nuclease target site

Mi{DR-white.3xP3-DsRed}MI00257-DR-white

RS(I-SceI): nuclease target site

Mi{DR-white.3xP3-DsRed}MI03233-DR-white

RS(I-SceI): nuclease target site

Mi{DR-white.3xP3-DsRed}MI03854-DR-white

RS(I-SceI): nuclease target site

Mi{DR-white.3xP3-DsRed}nAChRα4^{MI02830-DR-white}

RS(I-SceI): nuclease target site

nAChRα4^{MI02830-DR-white}

Mi{DR-white.3xP3-DsRed}Nubpl^{MI00127-DR-white}

RS(I-SceI): nuclease target site

Nubpl^{MI00127-DR-white}

P{DEL-qtc}attP40

attB: integrase target site
RS(I-CreI): nuclease target site
RS(I-SceI): nuclease target site

P{FRT(X97,I-site)RS3r}51F

FRT: recombinase target site
RS(I-SceI): nuclease target site

P{FRT(X97,I-site)RS3r}75CD

FRT: recombinase target site
RS(I-SceI): nuclease target site

RS(I-SceI): nuclease target site

RS(I-SceI): nuclease target site

RS(I-SceI): nuclease target site

FRT: recombinase target site
RS(I-SceI): nuclease target site

FRT: recombinase target site
RS(I-SceI): nuclease target site

PBac{wIw.2kb-3'}VK00031

RS(I-SceI): nuclease target site

PBac{wIw.2kb-5'}VK00031

RS(I-SceI): nuclease target site

PBac{wIw.3.5kb}VK00031

RS(I-SceI): nuclease target site

PBac{wIw.50bp-3'}VK00031

RS(I-SceI): nuclease target site

PBac{wIw.500bp-3'}VK00031

RS(I-SceI): nuclease target site

PBac{wIw.500bp-5'}VK00031

RS(I-SceI): nuclease target site

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

RS(I-SceI)

Scer\SceI.RS

(FlyBase, 2018-)

Name Synonyms

I-SceI recognition site

Secondary FlyBase IDs

FBgn0027366

References (40)

Report Sections