3xP3 regulatory sequences drive expression of EGFP. An in-frame RS(I-SceI) site has been introduced into the EGFP open reading frame.
A frameshift has been introduced into an internal NcoI site, inactivating the mini-w marker.
The mini-w gene has been inactivated by introducing a frameshift into an internal NcoI site.
Target construct for homing.
The mini-w gene has been inactivated by introducing a frameshift into an internal NcoI site.
Target construct for homing.