FlyBase Insertion Report: Dmel\P{PZ}smash[07735]

General Information

Symbol

Dmel\P{PZ}smash⁰⁷⁷³⁵

Species

D. melanogaster

Name

FlyBase ID

FBti0003560

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

ms(3)07735

Affected gene(s)

Ecol\lacZ, shk, smash

Viability / fertility

Associated allele(s)

Ecol\lacZ⁰⁷⁷³⁵, shk⁰⁷⁷³⁵, smash⁰⁷⁷³⁵

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3R ( 82D1 )

Sequence Location

3R:4,659,601..4,659,601 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3R:4,659,601..4,659,601 [-]

(Gene Disruption Project members, 2015.10.8)

Orientation

(Gene Disruption Project members, 2015.10.8)

Cytological location (computed by FlyBase)

82D1 (inferred by FlyBase from sequence location)

Cytological location (reported)

82D1_r6 (reported as inferred from sequence location)

(Gene Disruption Project members, 2015.10.8)

82C1-82C5 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

KS308186

(Gene Disruption Project members, 2015.10.8)

KS308185

(Gene Disruption Project members, 2015.10.8)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Braun et al., 1997, Castrillon et al., 1993)

shk

(FlyBase, 1996-, Castrillon et al., 1993)

smash

(Gene Disruption Project members, 2015.10.8, FlyBase, 1996-)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ⁰⁷⁷³⁵

Phenotypic Class Phenotype Manifest in Detailed Description

shk⁰⁷⁷³⁵

Phenotypic Class Phenotype Manifest in Detailed Description

smash⁰⁷⁷³⁵

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

Additional Information

Statement

Reference

In climbing third instar larva, high levels of expression in the salivary glands and trachea; moderate levels in the gut and epidermis.

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

ms(3)07735

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11796

P{PZ}smash⁰⁷⁷³⁵ shk⁰⁷⁷³⁵ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

External Crossreferences and Linkouts ( 0 )

Comments

The P{PZ}CG43427⁰⁷⁷³⁵ insertion maps within the CG43427 transcription unit. It is not yet known whether or not the sterility associated with the chromosome maps to the insertion. Thus shk⁰⁷⁷³⁵ (representing the sterility) has been kept as a separate allele in FlyBase for now.

(FlyBase, 1996-)

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

P1796

(Meister and Braun, 1995.10)

P{PZ}CG43427⁰⁷⁷³⁵

P{PZ}shk1

(Ostrowski et al., 2002)

P{PZ}shk¹

(Castrillon et al., 1993, FlyBase, 1992-)

P{PZ}smash⁰⁷⁷³⁵

P{ry^+t7.2=PZ}shk¹

(FlyBase, 1992-)

ms(3)07735

(Gene Disruption Project members, 2015.10.8)

Name Synonyms

Secondary FlyBase IDs

FBti0000735

References (12)

Report Sections

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