McLamarrah, T.A., Speed, S.K., Ryniawec, J.M., Buster, D.W., Fagerstrom, C.J., Galletta, B.J., Rusan, N.M., Rogers, G.C. (2020). A molecular mechanism for the procentriole recruitment of Ana2. J. Cell Biol. 219(2): e201905172.
FlyBase ID
FBrf0246771
Publication Type
Research paper
Abstract
During centriole duplication, a preprocentriole forms at a single site on the mother centriole through a process that includes the hierarchical recruitment of a conserved set of proteins, including the Polo-like kinase 4 (Plk4), Ana2/STIL, and the cartwheel protein Sas6. Ana2/STIL is critical for procentriole assembly, and its recruitment is controlled by the kinase activity of Plk4, but how this works remains poorly understood. A structural motif called the G-box in the centriole outer wall protein Sas4 interacts with a short region in the N terminus of Ana2/STIL. Here, we show that binding of Ana2 to the Sas4 G-box enables hyperphosphorylation of the Ana2 N terminus by Plk4. Hyperphosphorylation increases the affinity of the Ana2-G-box interaction, and, consequently, promotes the accumulation of Ana2 at the procentriole to induce daughter centriole formation.
