Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.
"CCCD" construct, coordinates relative to ana2-PA
Interaction in vitro; protein produced as a recombinant fusion proteins in bacterial system.
The ana2 CCCD region forms a very stable tetramer in vitro at a wide range of concentrations (36-900 uM).
"CCCD" construct, coordinates relative to ana2-PA
Interaction in vitro; protein produced as a recombinant fusion proteins in bacterial system.
The ana2 CCCD region forms a parallel, symmetrical 4-helix bundle.
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
coordinates relative to ana2-PA
coordinates relative to ana2-PA
Source was bacterial lysate; proteins coproduced as a recombinant fusion proteins.
coordinates relative to ana2-PA
coordinates relative to ana2-PA
Source was bacterial lysate; proteins coproduced as a recombinant fusion proteins.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
ana2 oligomerizes and was thus depleted by RNAi to eliminate its effect on the binding reaction.
T33E and S38D; coordinates relative to ana2-PA
amino acids mutated to aspartate or glutamate; coordinates relative to ana2-PA
Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.
Two-hybrid system: yeast GAL4-BD/VP16-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
