Source was cell extract of C131 cell line; bait produced from transfected construct; prey produced from endogenous gene.

This interaction is not affected by the L841K mutation.

Constructs were expressed in acentrosomal cell line derived from Sas-4 null homozygous mutant flies.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Smaller regions of the TCP domain (i.e., aa 771-813) were not sufficient for binding in this assay.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Both SAK-phosphorylated and non-phosphorylated forms of ana2 bind to Sas-4.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

ana2 was treated with functional or kinase-dead SAK to assess the effects of SAK phosphorylation on ana2 binding of other proteins.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

The ana2-Sas-4 interaction is increased upon coexpression of a stabilized version of SAK. This increase was not observed with a non-phosphorylatable S38A mutant of ana2 suggesting Sas-4 binds more strongly to phosphorylated ana2.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Tightest Sas-4-ana2 binding demonstrated with Sas-4 G-box and pre-phosphorylated full-length ana2 with a Kd of 6.7nM

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct or endogenous gene.

Coexpression of SAK increases the Sas-4-ana2 interaction in a manner that requires its kinase activity.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

ana2 oligomerizes and was thus depleted by RNAi to eliminate its effect on the binding reaction.