FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Sas-4, SAK
FlyBase ID
FBig0000120588
Interaction Network
Interactions Browser links
Sas-4 network
SAK network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0246771-12.coIP.WB
Reference
McLamarrah et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
SAK 

Plk4

bait

GFP tag

SAK 
Sas-4 

Sas4

prey

His tag

Sas-4 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
SAK 
interaction domain
sufficient binding region
aa 381-602

coordinates relative to SAK-PA

Sas-4 
interaction domain
sufficient binding region
aa 708-901

coordinates relative to Sas-4-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was bacterial lysate; proteins coproduced as a recombinant fusion proteins.

(McLamarrah et al., 2020)
FBrf0246771-16.MST
Reference
McLamarrah et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
SAK 

Plk4

neutral component

His tag

SAK 
SAK 

Plk4

neutral component

His tag

SAK 
SAK 

Plk4

neutral component

His tag

Sas-4 
Sas-4 

Sas4

neutral component

MBP tag

Sas-4 

Sas4

neutral component

MBP tag

Sas-4 
Sas-4 

Sas4

neutral component

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
SAK 
interaction domain
sufficient binding region
aa 382-602

coordinates relative to SAK-PA

Sas-4 
G-box domain
sufficient binding region
aa 708-901

coordinates relative to Sas-4-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

(McLamarrah et al., 2020)

Kd = 28.2uM

(McLamarrah et al., 2020)
FBrf0246771-6.PD.WB
Reference
McLamarrah et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

Sas4

bait

His tag

Sas-4 
SAK 

Plk4

prey

GFP tag

SAK 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
SAK 
interaction domain
sufficient binding region
aa 381-602

coordinates relative to SAK-PA

Sas-4 
interaction domain
sufficient binding region
aa 708-901

coordinates relative to Sas-4-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was bacterial lysate; proteins coproduced as a recombinant fusion proteins.

(McLamarrah et al., 2020)
FBrf0255743-11.PD.A
Reference
Ábrahám et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
SAK 

Plk4

bait

MBP tag

Sas-4 

Sas4

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

SAK was de-phosphorylated by PpD3 prior to binding reaction with Sas-4.

(Ábrahám et al., 2023)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Ábrahám et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (2)