Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Interaction in vitro; bait produced by in vitro translation; prey produced and labeled by in vitro translation.
Interaction in vitro; bait produced by in vitro translation; prey produced and labeled by in vitro translation.
Source was cell extract of COS7 cell line; bait produced from transfected construct; prey produced from transfected construct.
G3599V and G3601V, coordinates relative to 3726 aa isoform
E3616A and Y3617A, coordinates relative to 3726 aa isoform
P3670A and N3671Y, coordinates relative to 3726 aa isoform
I3693A, coordinates relative to 3726 aa isoform
E3697A and E3698A, coordinates relative to 3726 aa isoform
R3630A and D3635Δ, coordinates relative to 3726 aa isoform
V3653A and D3654A, coordinates relative to 3726 aa isoform
R3662A and F3662A, coordinates relative to 3726 aa isoform
D3702A and Y3703A, coordinates relative to 3726 aa isoform
C3721A and R3722A, coordinates relative to 3726 aa isoform
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
