nc82, nc-82, mAb nc82
cytoskeletal protein critical for intact presynaptic active zone structure and normal-evoked neurotransmitter release at chemical synapses - establishes proximity between Ca2+ channels and vesicles to allow efficient transmitter release and patterned synaptic plasticity
Please see the JBrowse view of Dmel\brp for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Gene model reviewed during 5.48
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.42
Gene model reviewed during 5.56
Gene model reviewed during 6.46
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\brp using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
brp protein is expressed in larva and adults as discrete puncta in the presynatic active zone of the mushroom body alpha/beta, alpha'/beta' and gamma neurons and calyx.
brp protein labels the presynaptic terminals in the medulla and lamina. Co-expression of brp and ScerGAL4cry.PE labelled cell projections was observed in the medulla, corresponding to DN3 neurons, but not in the lamina.
brp localizes opposite to clusters of glutamate receptors in the neuromuscular junction of late-stage embryos.
In lamina cross section, expression of brp is largely restricted to the ring of R1-R6 terminals within the photoreceptor cartridge, and shows a punctate distribution. Using immunoelectron microscopy, immunosignals are localized to regions of the T-bar ribbon in photoreceptor tetrads, specifically at the edge of the ribbon's platform. It is also expressed in the neuropils of the central brain.
brp protein is expressed in the presynaptic active zone. In the larval neuromuscular junction, it defines the core of the active zone, being surrounded by clusters of Liprin-α and RhoGAP100F.
nc82 is used as a generic marker for neuropil structures.
JBrowse - Visual display of RNA-Seq signals
View Dmel\brp in JBrowse2-60
2-59.2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
monoclonal
brp plays a role in promoting calcium channel clustering at the active zone.
RNAi knockdown flies show synaptic defects, impairment of walking behaviour and unstable flight.
Encodes a protein which is expressed in the neuropil structures of the adult brain.
Source for merge of: brp CG12933 CG30336 CG30337
Source for merge of: brp Mabnc82
Source for merge of: brp CG12932
Annotations CG34146 and CG12932 merged as CG42344 in release 5.9 of the genome annotation.
Annotations CG12933, CG30336 and CG30337 merged as CG34146 (which corresponds to brp) in release 5.1 of the genome annotation.
Annotation CG1931 split into CG30337 and CG30336 in release 3 of the genome annotation.
Named "bruchpilot" as mutants for this gene display unstable flight and quickly crash to the ground.
'bruchpilot' is German for 'crash pilot'.
