FlyBase Gene Report: Dmel\CG17575

General Information

Symbol

Dmel\CG17575

Species

D. melanogaster

Name

Annotation Symbol

CG17575

Feature Type

protein_coding_gene

FlyBase ID

FBgn0250842

Gene Model Status

Current

Stock Availability

6 publicly available

Gene Summary

CG17575 encodes a cysteine-rich secretory protein produced in the male accessory glands and transferred to females during mating. In mated females, it is required for the binding of the product of SP to sperm and for the consequent maintenance of long-term post-mating responses. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Interactive Fly

Also Known As

anon- SAGE:Wang-113

Key Links

Genomic Location

Cytogenetic map

49D4-49D4

Sequence location

2R:12,845,544..12,846,596 [-]

Recombination map

2-67

RefSeq locus

NT_033778 REGION:12845544..12846596

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (6 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (4 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in protein localization

inferred from mutant phenotype

(Ram and Wolfner, 2009)

involved_in regulation of female receptivity, post-mating

inferred from mutant phenotype

(Ram and Wolfner, 2007)

involved_in sexual reproduction

inferred from high throughput expression pattern

(Findlay et al., 2008, Mueller et al., 2005)

involved_in sperm competition

inferred from direct assay

(Avila and Wolfner, 2009)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in extracellular space

inferred from high throughput direct assay

(Findlay et al., 2008)

inferred from direct assay

(Walker et al., 2006)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in extracellular region

inferred from sequence model

(Kovalick and Griffin, 2005)

is_active_in extracellular space

inferred from biological aspect of ancestor with PANTHER:PTN000036124

(GO Reference Genome Project, 2011-)

located_in extracellular space

inferred from sequence model

(Mueller et al., 2005)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG17575 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A0A0B4JCT2)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG17575-RA

FBtr0087819

NM_136977

936

291

CG17575-RB

FBtr0302939

NM_001201988

957

298

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CG17575-PA

FBpp0086932

32.5

291

4.87

FBpp0292065

33.4

298

5.37

A0A0B4JCT2

NP_001188917

ADV37164

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG17575 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG17575_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.27

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

female reproductive system

Comment: mated females

(Ravi Ram et al., 2005)

adult hemolymph

Comment: mated females

(Ravi Ram et al., 2005)

uterus

Comment: mated females

(Ravi Ram et al., 2005)

oviduct

Comment: mated females

(Ravi Ram et al., 2005)

egg

Comment: mated females

(Ravi Ram et al., 2005)

Additional Descriptive Data

CG17575 is transferred to the female at the time of mating and persists for about 2-3 hours in the female reproductive tract. CG17575 enters the female hemolymph. It is detected in the uterus, mating plug, sperm mass, oviduct, and eggs.

(Ravi Ram et al., 2005)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in extracellular space

inferred from high throughput direct assay

(Findlay et al., 2008)

inferred from direct assay

(Walker et al., 2006)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CG17575 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG17575

View exonic expression by tissue for Dmel\CG17575

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0250842

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36414/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0250842

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0250842

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG17575

Flygut - An atlas of the Drosophila adult midgut

FBgn0250842

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG17575

Transgenic constructs containing regulatory region of CG17575

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Scer\GAL4^Mef2.PR

CG17575^GD5759

viable, with Scer\GAL4^pnr-MD237

CG17575^GD5759

viable, with Scer\GAL4^tin.CΔ4

CG17575^GD5759

Other Phenotypes

Allele

abnormal mating rhythm, with Scer\GAL4^αTub84B.PL

CG17575^RNAi.Sym.UAS

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (15)

Hsap\PI15

6 of 14

Yes

Hsap\R3HDML

6 of 14

Yes

5 of 14

5 of 14

5 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

Yes

4 of 14

3 of 14

3 of 14

3 of 14

2 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (13)

Rnor\Pi15

6 of 14

Yes

Rnor\R3hdml

6 of 14

Yes

5 of 14

5 of 14

5 of 14

5 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

2 of 14

2 of 14

Mus musculus (laboratory mouse) (15)

Mmus\Pi15

6 of 14

Yes

Mmus\R3hdml

6 of 14

Yes

5 of 14

5 of 14

5 of 14

5 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

3 of 14

2 of 14

Xenopus tropicalis (Western clawed frog) (21)

Xtro\crispld1

3 of 13

Yes

Xtro\crispld2

3 of 13

Yes

Xtro\pi15

3 of 13

Yes

Xtro\r3hdml

3 of 13

Yes

Xtro\crisp2-like

2 of 13

Xtro\LOC100490275

2 of 13

Xtro\crisp1.3

1 of 13

Yes

Xtro\crisp1.4

1 of 13

Yes

Xtro\crisp1.6

1 of 13

Yes

Xtro\crisp1.7

1 of 13

Yes

Xtro\crisp1.11

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (13)

Drer\pi15a

6 of 14

Yes

Drer\r3hdml

6 of 14

Yes

5 of 14

5 of 14

5 of 14

5 of 14

4 of 14

3 of 14

3 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (36)

Cele\scl-23

7 of 14

Yes

6 of 14

6 of 14

6 of 14

6 of 14

6 of 14

6 of 14

6 of 14

6 of 14

6 of 14

6 of 14

6 of 14

6 of 14

6 of 14

6 of 14

6 of 14

6 of 14

6 of 14

5 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Yes

Cele\Y39G8B.10

1 of 14

Yes

Cele\Y46C8AM.1

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (22)

Agam\AgaP_AGAP000485

7 of 12

Yes

Agam\AgaP_AGAP004893

7 of 12

Yes

Agam\AgaP_AGAP006417

7 of 12

Yes

Agam\AgaP_AGAP006418

7 of 12

Yes

Agam\AgaP_AGAP006419

7 of 12

Yes

Agam\AgaP_AGAP006421

7 of 12

Yes

Agam\AgaP_AGAP006443

7 of 12

Yes

Agam\AgaP_AGAP010550

7 of 12

Yes

Agam\AgaP_AGAP000356

6 of 12

Yes

Agam\AgaP_AGAP000357

6 of 12

Yes

6 of 12

6 of 12

6 of 12

5 of 12

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

Yes

Arabidopsis thaliana (thale-cress) (24)

Atha\AT3G19690

3 of 13

Yes

Atha\AT5G02730

3 of 13

Yes

Atha\PR1

3 of 13

Yes

Atha\PRB1

3 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

Yes

Atha\AT3G50685

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\PRY2

3 of 13

Yes

Scer\PRY1

2 of 13

Scer\PRY3

2 of 13

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CG17575. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (37)

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

9 of 13

8 of 13

8 of 13

8 of 13

8 of 13

7 of 13

7 of 13

6 of 13

6 of 13

5 of 13

5 of 13

5 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

3 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PI15; peptidase inhibitor 15

6 of 14

607076

R3HDML; R3H domain containing like

6 of 14

CRISPLD1; cysteine rich secretory protein LCCL domain containing 1

5 of 14

CRISPLD2; cysteine rich secretory protein LCCL domain containing 2

5 of 14

612434

GLIPR1L1; GLIPR1 like 1

5 of 14

610395

CRISP1; cysteine rich secretory protein 1

4 of 14

601193

CRISP2; cysteine rich secretory protein 2

4 of 14

187430

CRISP3; cysteine rich secretory protein 3

4 of 14

618062

GLIPR1; GLI pathogenesis related 1

4 of 14

602692

GLIPR1L2; GLIPR1 like 2

4 of 14

610394

PI16; peptidase inhibitor 16

4 of 14

CLEC18A; C-type lectin domain family 18 member A

3 of 14

616571

CLEC18B; C-type lectin domain family 18 member B

3 of 14

616572

CLEC18C; C-type lectin domain family 18 member C

3 of 14

616573

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

62186

DroID - A comprehensive database of gene and protein interactions.

FBgn0250842

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-67

Cytogenetic map

49D4-49D4

Sequence location

2R:12,845,544..12,846,596 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

49D4-49D4

Limits computationally determined from genome sequence between P{PZ}Nacα⁰⁴³²⁹ and P{lacW}bic^k10712

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-67

(FlyBase, 2016)

2-70.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (6)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

62888

y¹ v¹; P{TRiP.HMJ24128}attP40/CyO

55958

y¹ sc^* v¹ sev²¹; P{TRiP.HMC04247}attP40

NIG-Fly

HMJ24128

y¹ v¹; P{TRiP.HMJ24128}attP40/CyO

2RG-0372

y² VhaAC39-1^cho-1 v¹; P{U6-CG17575.NIG.sgRNA}attP40/CyO

VDRC

v105323

P{KK110897}VIE-260B

v30900

w¹¹¹⁸; P{GD5759}v30900

Genomic Clones (12)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (4)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

AT08432

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0250842

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

AT08444

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0250842

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

CG17575 is essential for sustained egg-laying, reduced receptivity of the mated female, and for the release of sperm from storage.

(Ram and Wolfner, 2007)

Isolated in a screen for seminal fluid proteins.

(Mueller et al., 2004)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG17575 anon- SAGE:Wang-113

(FlyBase, 1996-)

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (2)

Reported As

Name Synonyms

anon- SAGE:Wang-113

Secondary FlyBase IDs

FBgn0033776
FBgn0066885

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 33 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36414

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A0A0B4JCT2

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A0A0B4JCT2

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0250842

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36414/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0250842

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0250842

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG17575

MARRVEL_MODEL - MARRVEL (model organism gene)

36414

Linkouts

BioGRID - A database of protein and genetic interactions.

62186

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0250842

DroID - A comprehensive database of gene and protein interactions.

FBgn0250842

DRSC - Results frm RNAi screens

FBgn0250842

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG17575_1

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0250842

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG17575

Flygut - An atlas of the Drosophila adult midgut

FBgn0250842

References (55)

The following external sites may use different assemblies or annotations than FlyBase.

0.27

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