FB2024_03 , released June 25, 2024
Clone: Dmel\Nfly-113
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General Information
Symbol
Dmel\Nfly-113
Species
D. melanogaster
Name
Nfly-113
FlyBase ID
FBcl0006403
Feature type
Computed gene(s)
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    CB_cDNA
    • CB cDNA library of D. melanogaster, iso-1 strain, mixed stages.
    Strain
      Vector
      Tissue Source
      Sequence Data of the Insert
      Partial Sequence
      Total bases
      232
      NCBI
      Partial sequence dataSequence Downloader
      TTTTTTTTTTAAAGTCCTTTCATTTTTATTAAAGTTAGTGATTGGCCTTGGAATTGCAGAGTCCGCCAGTTCAGAAGAGG
      CCAGCCAGGTTGAGAAGTCGTAGATCTCGTTGACGGAGCACAGGTTAATAAAAACCGGATTCCGTCCGCTGCGGCATTCG
      GTGGCCGGACGATCGCCGCTCCGGTAGACCGGGGCATTGACATCATTTGTCCGAACAAAGTTGCAGGTCATG
      
      Library Information (1)
      Library: CB_cDNA
      Description

      Partial cDNAs generated with the GLGI technique; extend from the 3' poly(A) tail to the first CATG.

      Sample preparation

      A combination of embryos, second and third larvae, third day pupae, and young and aged adults, irradiated embryos and testis were used.

      Protocol

      Four SAGE libraries were constructed: (1) pooled sample including equal amounts of total RNA from embryos, second and third larvae, third day pupae, and young and aged adults, (2) embryos, (3) irradiated embryos and (4) testis. Unmatched (to annotated transcripts) SAGE tags from the four libraries were converted into 3' cDNAs using the GLGI (generation of longer 3' cDNA from SAGE tag for gene identification) method. Sequences extend from the poly(A) tail to the first CATG site.

      Comment

      GenBank CB305186-CB305318.

      Reported As
      Symbol Synonyms
      CB
      CB_cDNA
      References (1)
      Research paper (1)
      Lee et al., 2005, RNA 11(6): 939--946
      Detecting novel low-abundant transcripts in Drosophila. [FBrf0188306]
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)