FB2024_03 , released April 23, 2024
Gene: Dmel\Dlst
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General Information
Symbol
Dmel\Dlst
Species
D. melanogaster
Name
Dihydrolipoamide S-succinyltransferase
Annotation Symbol
CG5214
Feature Type
FlyBase ID
FBgn0037891
Gene Model Status
Stock Availability
Enzyme Name (EC)
2-oxoglutarate dehydrogenase system (1.2.1.105)
dihydrolipoyllysine-residue succinyltransferase (2.3.1.61)
Gene Summary
Contribute a Gene Snapshot for this gene.
Key Links
Genomic Location
Cytogenetic map
Sequence location
Recombination map
3-50
RefSeq locus
NT_033777 REGION:11244302..11246862
Sequence
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
Gene Ontology (GO) Annotations (8 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR006255
inferred from biological aspect of ancestor with PANTHER:PTN000567956
inferred from sequence or structural similarity with UniProtKB:P36957
inferred from sequence or structural similarity with UniProtKB:Q9N0F1
Biological Process (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from mutant phenotype
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from sequence or structural similarity with UniProtKB:P36957
traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000567956
inferred from electronic annotation with InterPro:IPR006255
Cellular Component (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in Z disc
inferred from direct assay
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
is_active_in mitochondrion
inferred from biological aspect of ancestor with PANTHER:PTN000567957
located_in mitochondrion
inferred from sequence or structural similarity with UniProtKB:P36957
inferred from sequence or structural similarity with UniProtKB:P36957
non-traceable author statement
inferred from sequence or structural similarity with UniProtKB:P36957
inferred from electronic annotation with InterPro:IPR006255
Protein Family (UniProt)
-
Catalytic Activity (EC/Rhea)
dihydrolipoyllysine-residue succinyltransferase activity
N(6)-[(R)-dihydrolipoyl]-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2] + succinyl-CoA = CoA + N(6)-[(R)-S(8)- succinyldihydrolipoyl]-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2] (2.3.1.61)
RHEA 15213: oxoglutarate dehydrogenase (NAD+) activity
2-oxoglutarate + CoA + NAD(+) = CO2 + NADH + succinyl-CoA (1.2.1.105)
RHEA 27786:
Summaries
Gene Group (FlyBase)
SUCCINYLTRANSFERASES -
Succinyltransferases catalyze the transfer of succinyl (3-carboxypropanoyl) group to an acceptor molecule.
OXOGLUTARATE DEHYDROGENASE COMPLEX - OGDH1 VARIANT -
The oxoglutarate dehydrogenase complex catalyses the conversion of 2-oxoglutarate into succinyl-CoA in the tricarboxylic acid cycle, thereby fulfilling crucial functions in mitochondria by providing metabolites and reduced electron carriers for oxidative phosphorylation. It is a giant enzyme cluster composed of multiple copies of three subunits. The dihydrolipoyllysine-residue succinyltransferase subunit (DLST, also known as E2) serves as a structural core unit. The OGDH (also known as E1) and dihydrolipoyl dehydrogenase (DLD, also known as E3) subunits sit in the periphery of the E2 core. The OGDH subunit recognizes the 2-oxoglutarate substrate and provides specificity to the complex. (Adapted from FBrf0256925.)
OXOGLUTARATE DEHYDROGENASE COMPLEX - OGDH2 VARIANT -
The oxoglutarate dehydrogenase complex catalyses the conversion of 2-oxoglutarate into succinyl-CoA in the tricarboxylic acid cycle, thereby fulfilling crucial functions in mitochondria by providing metabolites and reduced electron carriers for oxidative phosphorylation. It is a giant enzyme cluster composed of multiple copies of three subunits. The dihydrolipoyllysine-residue succinyltransferase subunit (DLST, also known as E2) serves as a structural core unit. The OGDH (also known as E1) and dihydrolipoyl dehydrogenase (DLD, also known as E3) subunits sit in the periphery of the E2 core. The OGDH subunit recognizes the 2-oxoglutarate substrate and provides specificity to the complex. (Adapted from FBrf0256925.)
OXOADIPATE DEHYDROGENASE COMPLEX -
A multi-enzyme complex that catalyzes the oxidative decarboxylation of 2-oxoadipate to glutaryl-CoA, thereby acting in the final step of lysine and tryptophan catabolism within mitochondria. The complex comprises multiple copies of three enzymes referred to as E1, E2 and E3: 2-oxoadipate dehydrogenase (E1), dihydrolipoamide S-succinyltransferase (E2) and dihydrolipoamide dehydrogenase (E3). The 2-oxoadipate dehydrogenase (E1) component is analogous to the oxoglutarate dehydrogenase (E1) component of the OGDH complex, while the E2 and E3 components of the two complexes are identical. (Adapted from PMID:29191460, PMID:32695416, PMID:33045952.]
OXOADIPATE DEHYDROGENASES -
*D Oxoadipate dehydrogenases form the E1 component of the oxoadipate dehydrogenase complex that catalyzes the oxidative decarboxylation of 2-oxoadipate to glutaryl-CoA, thereby acting in the final step of lysine and tryptophan catabolism within mitochondria. (Adapted from PMID:33045952.)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Dlst for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VGQ1)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0082358
1817
468
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
FBpp0081834
49.9
468
10.02
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Dlst using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

2.13

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in Z disc
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Dlst in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 2 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Dlst
Transgenic constructs containing regulatory region of Dlst
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Inferred from location ( 0 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (4)
13 of 14
Yes
Yes
2 of 14
No
No
2 of 14
No
No
2 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (4)
13 of 14
Yes
Yes
2 of 14
No
No
2 of 14
No
No
1 of 14
No
No
Mus musculus (laboratory mouse) (4)
12 of 14
Yes
Yes
2 of 14
No
No
2 of 14
No
No
2 of 14
No
No
Xenopus tropicalis (Western clawed frog) (4)
6 of 13
Yes
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Danio rerio (Zebrafish) (4)
13 of 14
Yes
Yes
2 of 14
No
No
2 of 14
No
No
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (4)
13 of 14
Yes
Yes
2 of 14
No
No
2 of 14
No
No
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (3)
10 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (8)
12 of 13
Yes
Yes
12 of 13
Yes
Yes
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (3)
12 of 13
Yes
Yes
2 of 13
No
No
2 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (3)
11 of 12
Yes
Yes
2 of 12
No
No
1 of 12
No
No
Escherichia coli (enterobacterium) (2)
11 of 11
Yes
Yes
3 of 11
No
No
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Dlst. Refer to their site for version information.
Paralogs
Paralogs (via DIOPT v9.1)
Drosophila melanogaster (Fruit fly) (3)
3 of 13
3 of 13
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Show/hide secondary interactors 
    (data from AllianceMine provided by esyN)
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Other Interaction Browsers

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    Show/hide secondary interactors 
    (data from AllianceMine provided by esyN)
    esyN Network Key:
    Suppression
    Enhancement
    Other Interaction Browsers

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
    Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
    External Data
    Linkouts
    KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3R
    Recombination map
    3-50
    Cytogenetic map
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    86D9-86D9
    Limits computationally determined from genome sequence between P{EP}EP3340EP3340 and P{PZ}tho1&P{PZ}l(3)0462905275
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (8)
    Genomic Clones (21)
    cDNA Clones (94)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Cell Line Information
    Publicly Available Cell Lines
     
      Other Stable Cell Lines
       
        Other Comments
        Relationship to Other Genes
        Source for database merge of
        Additional comments
        Nomenclature History
        Source for database identify of

        Source for identity of: Dlst CG5214

        Nomenclature comments
        Etymology

        Named 'Dlst' after the human ortholog.

        Synonyms and Secondary IDs (8)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        Study result (0)
        Result
        Result Type
        Title
        External Crossreferences and Linkouts ( 60 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
        DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
        EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
        FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
        FlyMine - An integrated database for Drosophila genomics
        KEGG Genes - Molecular building blocks of life in the genomic space.
        MARRVEL_MODEL - MARRVEL (model organism gene)
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
        References (65)