FB2024_04 , released June 25, 2024
Gene: Dmel\asf1
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General Information
Symbol
Dmel\asf1
Species
D. melanogaster
Name
anti-silencing factor 1
Annotation Symbol
CG9383
Feature Type
protein_coding_gene
FlyBase ID
FBgn0029094
Gene Model Status
Current
Stock Availability
11 publicly available
Gene Summary
Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly (PubMed:10591219, PubMed:12381660, PubMed:14561777, PubMed:16151251, PubMed:16396992). Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly (PubMed:11533245). Plays a role in the formation of silent heterochromatin (PubMed:12381660, PubMed:28320872). (UniProt, Q9V464)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

dASF1

Key Links
Genomic Location
Cytogenetic map
76B9-76B9
Sequence location
Recombination map
3-45
RefSeq locus
NT_037436 REGION:19618894..19620398
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (12 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables protein binding
inferred from physical interaction with UniProtKB:P25439
(Moshkin et al., 2002)
inferred from physical interaction with UniProtKB:Q9VF03
(Moshkin et al., 2002)
inferred from physical interaction with FLYBASE:Tlk; FB:FBgn0283657
(Carrera et al., 2003)
inferred from physical interaction with UniProtKB:Q24572
(Tyler et al., 2001)
inferred from physical interaction with FLYBASE:Bx42; FB:FBgn0004856
(Goodfellow et al., 2007)
inferred from physical interaction with UniProtKB:P02299
(Tyler et al., 1999)
inferred from physical interaction with UniProtKB:P84040
(Tyler et al., 1999)
enables RNA polymerase II-specific DNA-binding transcription factor binding
inferred from physical interaction with FLYBASE:H; FB:FBgn0001169
(Goodfellow et al., 2007)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables histone binding
inferred from biological aspect of ancestor with PANTHER:PTN000247832
(GO Reference Genome Project, 2011-)
Biological Process (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
involved_in chromatin organization
inferred from direct assay
(Tyler et al., 1999)
involved_in DNA replication-dependent chromatin assembly
inferred from mutant phenotype
(Tyler et al., 1999)
involved_in negative regulation of transcription by RNA polymerase II
inferred from mutant phenotype
(Goodfellow et al., 2007)
involved_in regulation of mRNA splicing, via spliceosome
inferred from mutant phenotype
(Blanchette et al., 2005)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in chromatin organization
inferred from electronic annotation with InterPro:IPR006818, InterPro:IPR036747
(InterPro Project Members, 2004-)
involved_in DNA replication-dependent chromatin assembly
inferred from biological aspect of ancestor with PANTHER:PTN000247832
(GO Reference Genome Project, 2011-)
Cellular Component (5 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
located_in polytene chromosome
inferred from direct assay
(Goodfellow et al., 2007)
part_of RCAF complex
inferred from direct assay
(Tyler et al., 2001)
inferred from direct assay
(Tyler et al., 1999)
part_of transcription repressor complex
inferred from direct assay
(Goodfellow et al., 2007)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
part_of chromatin
inferred from biological aspect of ancestor with PANTHER:PTN000247832
(GO Reference Genome Project, 2011-)
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000247832
(GO Reference Genome Project, 2011-)
located_in nucleus
inferred from electronic annotation with InterPro:IPR006818, InterPro:IPR036747
(InterPro Project Members, 2004-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the ASF1 family. (Q9V464)
Protein Signatures (InterPro)
Summaries
Protein Function (UniProtKB)
Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly (PubMed:10591219, PubMed:12381660, PubMed:14561777, PubMed:16151251, PubMed:16396992). Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly (PubMed:11533245). Plays a role in the formation of silent heterochromatin (PubMed:12381660, PubMed:28320872).
(UniProt, Q9V464)
Summary (Interactive Fly)

histone chaperone that assists in chromatin assembly and remodeling during replication, transcription activation, and gene silencing - contributes to the repression of Notch target genes - directly interacts Hairless

Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\asf1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9V464)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
asf1-RA
FBtr0074947
NM_079439
1505
218
asf1-RB
FBtr0302381
NM_001202202
1129
218
Additional Transcript Data and Comments
Reported size (kB)

1.611 (compiled cDNA)

(Tyler et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
asf1-PA
FBpp0074715
24.4
218
4.23
Q9V464
NP_524163
AAF49131
asf1-PB
FBpp0291576
24.4
218
4.23
Q9V464
NP_001189131
ADV37567
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

218 aa isoforms: asf1-PA, asf1-PB
Additional Polypeptide Data and Comments
Reported size (kDa)

218 (aa)

(Tyler et al., 1999)
Comments
External Data
Subunit Structure (UniProtKB)

Component of the replication coupling assembly factor (RCAF), composed of asf1, histone H3 and histone H4 (PubMed:10591219). Interacts with the Caf1-105 subunit of the CAF-1 complex (PubMed:11533245). Interacts with brm, mor and tlk (PubMed:12381660, PubMed:14561777). Interacts with Daxx (PubMed:28320872).

(UniProt, Q9V464)
Post Translational Modification

Phosphorylated on undefined residues by tlk.

(UniProt, Q9V464)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\asf1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.28

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 7 -- 8
anterior endoderm anlage
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 9 -- 10
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
anterior endoderm
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 11 -- 12
anterior midgut primordium
(Fisher et al., 2012)
central brain primordium
(Fisher et al., 2012)
posterior midgut primordium
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic central brain surface glial cell
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage -- adult stage
(Tyler et al., 1999)
Additional Descriptive Data

asf1 protein is highly abundant in embryos and less abundant in larvae, pupae, and adults.

(Tyler et al., 1999)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in polytene chromosome
inferred from direct assay
(Goodfellow et al., 2007)
part_of RCAF complex
inferred from direct assay
(Tyler et al., 2001)
inferred from direct assay
(Tyler et al., 1999)
part_of transcription repressor complex
inferred from direct assay
(Goodfellow et al., 2007)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\asf1 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 5 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of asf1
Transgenic constructs containing regulatory region of asf1
Aberrations (Deficiencies and Duplications) ( 27 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4Act5C.PI
lethal, with Scer\GAL4ey.PH
lethal | female limited, with Scer\GAL4dome-PG14
lethal | recessive
lethal - all die before end of larval stage (with Df(3L)kto2)
lethal - all die before end of larval stage | heat sensitive, with Scer\GAL4hs.2sev
partially lethal - majority die, with Scer\GAL4nub-AC-62
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
female sterile, with Scer\GAL4VP16.otu
female sterile | germline clone
Other Phenotypes
Allele
some die during larval stage (with Df(3L)kto2)
some die during larval stage | heat sensitive, with Scer\GAL4hs.2sev
suppressor of variegation | dominant, with In(1)wm4h
suppressor of variegation | dominant, with In(2)Heidi/+:
visible, with Scer\GAL4bbg-C96
visible, with Scer\GAL4ey.PH
visible, with Scer\GAL4nub-AC-62
visible, with Scer\GAL4pnr-MD237
Phenotype manifest in
Allele
embryonic/larval salivary gland, with Scer\GAL4hs.2sev
eye, with Scer\GAL4ey.PH
larval salivary gland & nucleus, with Scer\GAL4hs.2sev
macrochaeta, with Scer\GAL4hs.2sev
ovary | germline clone
trichogen cell, with Scer\GAL4pnr-MD237
wing, with Scer\GAL4bbg-C96
wing, with Scer\GAL4nub-AC-62
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (2)
Hsap\ASF1A
13 of 14
Yes
Yes
1  
Hsap\ASF1B
13 of 14
Yes
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (2)
Rnor\Asf1a
13 of 14
Yes
Yes
Rnor\Asf1b
13 of 14
Yes
Yes
Mus musculus (laboratory mouse) (2)
Mmus\Asf1a
14 of 14
Yes
Yes
Mmus\Asf1b
12 of 14
No
Yes
Xenopus tropicalis (Western clawed frog) (4)
Xtro\asf1b
11 of 13
Yes
Yes
Xtro\asf1a
7 of 13
No
Yes
Xtro\mdn1
1 of 13
No
No
Xtro\tll1
1 of 13
No
No
Danio rerio (Zebrafish) (2)
Drer\asf1bb
14 of 14
Yes
Yes
Drer\asf1ba
13 of 14
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\asfl-1
14 of 14
Yes
Yes
Cele\unc-85
13 of 14
No
Yes
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP005920
12 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (2)
Atha\ASF1B
13 of 13
Yes
Yes
Atha\SGA2
13 of 13
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\ASF1
13 of 13
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\cia1
11 of 12
Yes
Yes
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:asf1. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    ASF1A; anti-silencing function 1A histone chaperone
    13 of 14
    609189
         
        ASF1B; anti-silencing function 1B histone chaperone
        13 of 14
        609190
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            esyN Network Diagram
            Show neighbor-neighbor interactions:
            Show/hide secondary interactors 
            (data from AllianceMine provided by esyN)
            Select Layout:
            Legend:
            Protein
            RNA
            Selected Interactor(s)
            Other Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please see the Physical Interaction reports below for full details
            protein-protein
            Physical Interaction
            Assay
            References
            asf1 - CG15356
            anti bait coimmunoprecipitation, western blot, Identification by mass spectrometry
            (Moshkin et al., 2009)
            asf1 - CG3815
            anti bait coimmunoprecipitation, Identification by mass spectrometry, western blot
            (Moshkin et al., 2009)
            asf1 - Caf1-105
            anti bait coimmunoprecipitation, Identification by mass spectrometry, western blot
            (Moshkin et al., 2009)
            asf1 - Daxx
            anti tag coimmunoprecipitation, western blot
            (Fromental-Ramain et al., 2017)
            asf1 - H
            two hybrid, pull down, anti tag western blot, autoradiography, isothermal titration calorimetry, predetermined participant
            (Maier et al., 2023, Goodfellow et al., 2007)
            asf1 - Hira
            anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot, Identification by mass spectrometry
            (Horard et al., 2018, Moshkin et al., 2009)
            asf1 - His3
            cosedimentation through density gradient, molecular weight estimation by staining, tandem affinity purification, Identification by mass spectrometry, western blot
            (Fromental-Ramain et al., 2017, Tyler et al., 1999)
            asf1 - His3.3A
            tandem affinity purification, Identification by mass spectrometry, western blot
            (Fromental-Ramain et al., 2017)
            asf1 - His3.3B
            tandem affinity purification, western blot, Identification by mass spectrometry
            (Fromental-Ramain et al., 2017)
            asf1 - His4
            cosedimentation through density gradient, molecular weight estimation by staining
            (Tyler et al., 1999)
            asf1 - Kdm5
            anti bait coimmunoprecipitation, Identification by mass spectrometry, western blot
            (Moshkin et al., 2009)
            asf1 - MRG15
            anti bait coimmunoprecipitation, Identification by mass spectrometry, western blot
            (Moshkin et al., 2009)
            asf1 - Sin3A
            anti bait coimmunoprecipitation, western blot, Identification by mass spectrometry
            (Moshkin et al., 2009)
            asf1 - Su(H)
            anti bait coimmunoprecipitation, western blot
            (Moshkin et al., 2009, Goodfellow et al., 2007)
            asf1 - Tlk
            enzymatic study, autoradiography
            (Pilyugin et al., 2009)
            asf1 - Ubc6
            anti tag coimmunoprecipitation, western blot, pull down
            (Wang et al., 2015)
            asf1 - brm
            anti bait coimmunoprecipitation, western blot, pull down
            (Moshkin et al., 2002)
            asf1 - gro
            anti bait coimmunoprecipitation, western blot
            (Goodfellow et al., 2007)
            asf1 - hd
            experimental knowledge based
            (Guruharsha et al., 2011)
            asf1 - mor
            anti bait coimmunoprecipitation, western blot
            (Moshkin et al., 2002)
            asf1 - nej
            anti bait coimmunoprecipitation, western blot
            (Das et al., 2009)
            asf1 - p53
            pull down, autoradiography
            (Lunardi et al., 2010)
            Summary of Genetic Interactions
            esyN Network Diagram
            Show/hide secondary interactors 
            (data from AllianceMine provided by esyN)
            esyN Network Key:
            Suppression
            Enhancement
            Other Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            asf1
            suppressible
            brm
            (Moshkin et al., 2002)
            asf1
            suppressible
            Kdm5
            (Moshkin et al., 2009)
            asf1
            suppressible
            mor
            (Moshkin et al., 2002)
            asf1
            suppressible
            osa
            (Moshkin et al., 2002)
            asf1
            suppressible
            Sin3A
            (Moshkin et al., 2009)
            asf1
            enhanceable
            Tlk
            (Carrera et al., 2003)
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            brm
            enhanceable
            asf1
            (Moshkin et al., 2002)
            bw
            suppressible
            asf1
            (Schneiderman et al., 2010)
            Caf1-180
            suppressible
            asf1
            (Song et al., 2007)
            dom
            enhanceable
            asf1
            (Ellis et al., 2015)
            Tlk
            enhanceable
            asf1
            (Carrera et al., 2003)
            Tlk
            suppressible
            asf1
            (Carrera et al., 2003)
            External Data
            Subunit Structure (UniProtKB)
            Component of the replication coupling assembly factor (RCAF), composed of asf1, histone H3 and histone H4 (PubMed:10591219). Interacts with the Caf1-105 subunit of the CAF-1 complex (PubMed:11533245). Interacts with brm, mor and tlk (PubMed:12381660, PubMed:14561777). Interacts with Daxx (PubMed:28320872).
            (UniProt, Q9V464 )
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            External Data
            Linkouts
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            3L
            Recombination map
            3-45
            Cytogenetic map
            76B9-76B9
            Sequence location
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            76B9-76B9
            Limits computationally determined from genome sequence between P{lacW}l(3)L3809L3809 and P{lacW}l(3)L1243L1243&P{lacW}Mi-2j3D4
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            76C1-76C3
            (determined by in situ hybridisation)
            (Tyler et al., 1999)
            76C1-76C3
            (Tyler, 1999.9.23)
            Experimentally Determined Recombination Data
            Location

            3-45

            (FlyBase, 2016)

            3-43.1

            (Comeron et al., 2012)

            3-46.2

            (Moshkin et al., 2002)
            Left of (cM)
            (Moshkin et al., 2002)
            Right of (cM)
            (Moshkin et al., 2002)
            Notes
            Stocks and Reagents
            Stocks (11)
            Genomic Clones (16)
             

            Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

            cDNA Clones (91)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            Antibody Information
            Laboratory Generated Antibodies
             
            Commercially Available Antibodies
             
            Cell Line Information
            Publicly Available Cell Lines
             
              Other Stable Cell Lines
               
                Other Comments

                DNA-protein interactions: genome-wide binding profile assayed for asf1 protein in Kc167 cells; see Chromatin_types_NKI collection report. Individual protein-binding experiments listed under "Samples" at GEO_GSE22069 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22069).

                (Filion et al., 2010)

                dsRNA has been made from templates generated with primers directed against this gene. RNAi of asf1 results in increased arborization of ddaD and ddaE neurons, defects in muscle and defects in dendrite morphogenesis.

                (Parrish et al., 2006)

                The tlk-asf1 pathway is important for both DNA replication and chromatin assembly.

                (Carrera et al., 2003)

                asf1 may have a crucial role in both chromatin assembly and SWI/SNF-mediated chromatin remodelling.

                (Moshkin et al., 2002)

                Mutations in asf1 de-repress silencing at heterochromatin.

                (Moshkin et al., 2002)

                Replication-coupling assembly factor (RCAF) is a protein complex that facilitates the assembly of nucleosomes onto newly replicated DNA in vitro. RCAF is comprised of asf1, His3 and His4.

                (Tyler et al., 1999)
                Relationship to Other Genes
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                Additional comments
                Nomenclature History
                Source for database identify of
                Nomenclature comments
                Etymology
                Synonyms and Secondary IDs (12)
                Datasets (1)
                Study focus (1)
                Experimental Role
                Project
                Project Type
                Title
                • transgene_used
                FBGSE22069
                Protein profiling reveals five principal chromatin types in Drosophila cells.
                Study result (0)
                Result
                Result Type
                Title
                External Crossreferences and Linkouts ( 40 )
                Sequence Crossreferences
                NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                Other crossreferences
                AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                FlyMine - An integrated database for Drosophila genomics
                InterPro - A database of protein families, domains and functional sites
                KEGG Genes - Molecular building blocks of life in the genomic space.
                MARRVEL_MODEL - MARRVEL (model organism gene)
                Linkouts
                BioGRID - A database of protein and genetic interactions.
                Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                DroID - A comprehensive database of gene and protein interactions.
                DRSC - Results frm RNAi screens
                Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                Flygut - An atlas of the Drosophila adult midgut
                iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                MIST (genetic) - An integrated Molecular Interaction Database
                MIST (protein-protein) - An integrated Molecular Interaction Database
                References (86)