FlyBase Gene Report: Dmel\Cbp53E

General Information

Symbol

Dmel\Cbp53E

Species

D. melanogaster

Name

Calbindin 53E

Annotation Symbol

CG6702

Feature Type

protein_coding_gene

FlyBase ID

FBgn0004580

Gene Model Status

Current

Stock Availability

25 publicly available

Gene Summary

Calbindin 53E (Cbp53E) encodes a 6 EF-hand domain-containing protein that regulates neuronal development. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Interactive Fly

Also Known As

cbn, calbindin-32, calbindin

Key Links

Genomic Location

Cytogenetic map

53E4-53E4

Sequence location

2R:16,973,927..16,996,057 [+]

Recombination map

2-81

RefSeq locus

NT_033778 REGION:16973927..16996057

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (10 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables calcium ion binding

traceable author statement

(Harteneck, 2003)

inferred from electronic annotation with InterPro:IPR002048

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN001461922

(GO Reference Genome Project, 2011-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in regulation of long-term synaptic potentiation

inferred from biological aspect of ancestor with PANTHER:PTN000463232

(GO Reference Genome Project, 2011-)

involved_in regulation of presynaptic cytosolic calcium ion concentration

inferred from biological aspect of ancestor with PANTHER:PTN000463232

(GO Reference Genome Project, 2011-)

Cellular Component (7 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in type II terminal bouton

inferred from direct assay

(Hagel et al., 2015)

located_in type III terminal bouton

inferred from direct assay

(Hagel et al., 2015)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

is_active_in cytosol

inferred from biological aspect of ancestor with PANTHER:PTN001461922

(GO Reference Genome Project, 2011-)

is_active_in dendrite

inferred from biological aspect of ancestor with PANTHER:PTN000463232

(GO Reference Genome Project, 2011-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN001461922

(GO Reference Genome Project, 2011-)

is_active_in synapse

inferred from biological aspect of ancestor with PANTHER:PTN001461922

(GO Reference Genome Project, 2011-)

is_active_in terminal bouton

inferred from biological aspect of ancestor with PANTHER:PTN000463232

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Belongs to the calbindin family. (P41044)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Calbindin 53E (Cbp53E) encodes a 6 EF-hand domain-containing protein that regulates neuronal development. [Date last reviewed: 2019-03-07]

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Cbp53E for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P41044)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Cbp53E-RA

FBtr0087056

NM_057490

2705

310

Cbp53E-RB

FBtr0340072

NM_001299582

1400

310

Additional Transcript Data and Comments

Reported size (kB)

2.8, 1.7 (northern blot)

(Reifegerste et al., 1993)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Cbp53E-PA

FBpp0086204

35.7

310

4.32

FBpp0309078

35.7

310

4.32

P41044

NP_001286511

AHN56307

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

310 aa isoforms: Cbp53E-PA, Cbp53E-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

310 (aa)

(Reifegerste et al., 1993)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cbp53E using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.71

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

eye

(Xu et al., 2004)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic head sensory system

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

embryonic/larval nervous system

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

embryonic stage 15 -- 17

presumptive embryonic/larval central nervous system | restricted

Comment: reference states >12 hr AEL

(Reifegerste et al., 1993)

presumptive embryonic/larval peripheral nervous system | restricted

Comment: reference states >12 hr AEL

(Reifegerste et al., 1993)

neuron

Comment: reference states >12 hr AEL

(Reifegerste et al., 1993)

embryonic stage 17

ventral midline of embryo

(Reifegerste et al., 1993)

third instar larval stage

embryonic/larval central nervous system | restricted

(Reifegerste et al., 1993)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

(Hagel et al., 2015)

Additional Descriptive Data

Eye-enriched transcripts determined by ratio of expression level in wild-type heads. versus expression level in so heads.

(Xu et al., 2004)

In late embryos, Cbp53E expression is observed in segmental pairs of cells along the midline and in other cells of the nervous system. In third instar larvae, expression is observed in the perikarya of the proximal part of the two brain hemispheres but not in the lateral hemispheres which contain the optic lobes. Expression is also observed in cells throughout the thoracic and abdominal ganglia. In adults, expression is mainly observed in the nervous system. Cbp53E is expressed in a large number of neurons in the brain and thoracic ganglion. In neurons, it is expressed in both the perikarya and in the processes. In the visual system, staining is observed in the outer lamina and in medulla layer 2. Expression is also observed in the antennal system and in two small muscles in the thorax which are thought to be direct flight muscles.

(Reifegerste et al., 1993)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 15 -- 17

presumptive embryonic/larval central nervous system | restricted

Comment: reference states >12 hr AEL

(Reifegerste et al., 1993)

presumptive embryonic/larval peripheral nervous system | restricted

Comment: reference states >12 hr AEL

(Reifegerste et al., 1993)

neuron

Comment: reference states >12 hr AEL

(Reifegerste et al., 1993)

embryonic stage 17

ventral midline of embryo

(Reifegerste et al., 1993)

third instar larval stage

embryonic/larval central nervous system | restricted

(Reifegerste et al., 1993)

embryonic/larval brain | restricted

(Hagel et al., 2015)

embryonic/larval motor neuron | subset

(Hagel et al., 2015)

embryonic/larval neuromuscular junction | subset

(Hagel et al., 2015)

type II bouton | subset

(Hagel et al., 2015)

type III bouton | subset

(Hagel et al., 2015)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Cbp53E protein is detected in type III synaptic boutons at neuromuscular junctions (NMJs) with muscle 12 and in type II synaptic boutons at NMJs with muscles 4,6,7,12 and 13.

(Hagel et al., 2015)

(Reifegerste et al., 1993)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in type II terminal bouton

inferred from direct assay

(Hagel et al., 2015)

located_in type III terminal bouton

inferred from direct assay

(Hagel et al., 2015)

Expression Deduced from Reporters

Reporter: P{Cbp53E-GAL4.VP16.I}

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

interneuron | subset

(Bloomington Drosophila Stock Center, 2017)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Cbp53E in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Cbp53E

View exonic expression by tissue for Dmel\Cbp53E

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0004580

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36905/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004580

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004580

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004580

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6702

Flygut - An atlas of the Drosophila adult midgut

FBgn0004580

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Cbp53E

Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Cbp53E

Transgenic constructs containing regulatory region of Cbp53E

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2R)Exel6065

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Mef2.PR

Cbp53E^GD10584

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Cbp53E^GD10584

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Cbp53E^GD10584

viable, with Scer\GAL4^tin.CΔ4

Cbp53E^GD10584

Sterility

Allele

fertile

Cbp53E^f00876

Other Phenotypes

Allele

abnormal neuroanatomy | conditional

Cbp53E^f00876

abnormal neuroanatomy | third instar larval stage

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^how-24B

Cbp53E^UAS.cHa

abnormal neuroanatomy | third instar larval stage (with Cbp53E^Mi22)

Cbp53E^Mi41

abnormal neuroanatomy | third instar larval stage (with Cbp53E^Mi41)

Cbp53E^Mi22

abnormal neuroanatomy | third instar larval stage (with Df(2R)ED2751)

some die during pupal stage, with Scer\GAL4^pnr-MD237

Cbp53E^GD10584

visible, with Scer\GAL4^pnr-MD237

Cbp53E^GD10584

Phenotype manifest in

Allele

olfactory receptor neuron | conditional

Cbp53E^f00876

trichogen cell, with Scer\GAL4^pnr-MD237

Cbp53E^GD10584

type Ib terminal bouton | third instar larval stage

Cbp53E^Mi22

type Ib terminal bouton | third instar larval stage (with Cbp53E^Mi22)

Cbp53E^Mi41

type Ib terminal bouton | third instar larval stage (with Cbp53E^Mi41)

Cbp53E^Mi22

type Ib terminal bouton | third instar larval stage (with Df(2R)ED2751)

Cbp53E^Mi22

type II bouton | third instar larval stage

type II bouton | third instar larval stage, with Scer\GAL4^how-24B

Cbp53E^UAS.cHa

type II bouton | third instar larval stage (with Cbp53E^Mi22)

Cbp53E^Mi41

type II bouton | third instar larval stage (with Cbp53E^Mi41)

Cbp53E^Mi22

type II bouton | third instar larval stage (with Df(2R)ED2751)

type III bouton | third instar larval stage

type III bouton | third instar larval stage, with Scer\GAL4^how-24B

Cbp53E^UAS.cHa

type III bouton | third instar larval stage (with Cbp53E^Mi22)

Cbp53E^Mi41

type III bouton | third instar larval stage (with Cbp53E^Mi41)

Cbp53E^Mi22

type III bouton | third instar larval stage (with Df(2R)ED2751)

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (20)

Hsap\CALB2

12 of 14

Yes

Hsap\CALB1

11 of 14

Yes

Hsap\SCGN

8 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Hsap\LOC118142757

1 of 14

Hsap\NCALD

1 of 14

Hsap\OCM2

1 of 14

Yes

Hsap\OCM

1 of 14

Yes

Hsap\PVALB

1 of 14

Yes

Hsap\PVALEF

1 of 14

Yes

Hsap\SLC25A24

1 of 14

Hsap\TNNC1

1 of 14

Hsap\TNNC2

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (13)

Rnor\Calb2

12 of 14

Yes

Rnor\Calb1

11 of 14

Yes

Rnor\Scgn

8 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Pvalb

1 of 14

Yes

Rnor\Tnnc1

1 of 14

Rnor\Tnnc2

1 of 14

Mus musculus (laboratory mouse) (14)

Mmus\Calb2

12 of 14

Yes

Mmus\Calb1

10 of 14

Yes

Mmus\Scgn

8 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Pvalb

1 of 14

Yes

Mmus\Tnnc1

1 of 14

Mmus\Tnnc2

1 of 14

Xenopus tropicalis (Western clawed frog) (12)

Xtro\calb2

9 of 13

Yes

Xtro\scgn

7 of 13

Yes

Xtro\calb1

5 of 13

Yes

5 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (26)

Drer\scgn

11 of 14

Yes

Drer\calb2a

10 of 14

Yes

Drer\calb2b

10 of 14

Yes

Drer\calb1

8 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\pvalb2

1 of 14

Yes

Drer\pvalb3

1 of 14

Yes

Drer\pvalb4

1 of 14

Yes

Drer\pvalb5

1 of 14

Yes

Drer\pvalb6

1 of 14

Yes

Drer\pvalb7

1 of 14

Yes

Drer\pvalb8

1 of 14

Yes

Drer\pvalb9

1 of 14

Yes

Drer\tnnc1a

1 of 14

Drer\tnnc1b

1 of 14

Drer\tnnc2.1

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (7)

Cele\B0563.7

1 of 14

Yes

Cele\cal-1

1 of 14

Yes

Cele\cal-2

1 of 14

Yes

Cele\cal-4

1 of 14

Yes

Cele\cmd-1

1 of 14

Yes

Cele\pat-10

1 of 14

Yes

Cele\tnc-2

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (8)

Agam\AgaP_AGAP012179

12 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (32)

Atha\AT1G05990

1 of 13

Yes

Atha\AT1G18210

1 of 13

Yes

Atha\AT1G18530

1 of 13

Yes

Atha\AT1G32250

1 of 13

Yes

Atha\AT3G03000

1 of 13

Yes

Atha\AT3G10190

1 of 13

Yes

Atha\AT3G25600

1 of 13

Yes

Atha\AT3G47480

1 of 13

Yes

Atha\AT4G03290

1 of 13

Yes

Atha\CAM1

1 of 13

Yes

Atha\CAM2

1 of 13

Yes

Atha\CAM3

1 of 13

Yes

Atha\CAM4

1 of 13

Yes

Atha\CAM5

1 of 13

Yes

Atha\CAM6

1 of 13

Yes

Atha\CAM7

1 of 13

Yes

Atha\CAM8

1 of 13

Yes

Atha\CAM9

1 of 13

Yes

Atha\CDPK6

1 of 13

Yes

Atha\CML11

1 of 13

Yes

Atha\CML23

1 of 13

Yes

Atha\CML42

1 of 13

Yes

Atha\CML43

1 of 13

Yes

Atha\CPK2

1 of 13

Yes

Atha\CPK7

1 of 13

Yes

Atha\CPK9

1 of 13

Yes

Atha\CPK13

1 of 13

Yes

Atha\CPK15

1 of 13

Yes

Atha\CPK22

1 of 13

Yes

Atha\CPK32

1 of 13

Yes

Atha\TCH2

1 of 13

Yes

Atha\TCH3

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\CMD1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Cbp53E. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (5)

azot

1 of 13

Cam

1 of 13

CG17493

1 of 13

CG31802

1 of 13

Nca

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

12 of 14

11 of 14

SCGN; secretagogin, EF-hand calcium binding protein

8 of 14

609202

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Cbp53E - orb2

anti tag coimmunoprecipitation, Identification by mass spectrometry, western blot

(White-Grindley et al., 2014)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

fzr

suppressible

Cbp53E

(Neuert et al., 2017)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0004580

MIST (protein-protein) - An integrated Molecular Interaction Database

36905

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-81

Cytogenetic map

53E4-53E4

Sequence location

2R:16,973,927..16,996,057 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

53E4-53E4

Limits computationally determined from genome sequence between P{lacW}CG6426^k10209&P{lacW}veg^k07202 and P{lacW}l(2)k07408^k07408

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

53E-53E

(determined by in situ hybridisation)

(Reifegerste et al., 1993)

Experimentally Determined Recombination Data

Location

2-81

(FlyBase, 2016)

2-84.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (25)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

39403

w¹¹¹⁸; P{GMR67B03-GAL4}attP2

39406

w¹¹¹⁸; P{GMR67C01-GAL4}attP2

39410

w¹¹¹⁸; P{GMR67C09-GAL4}attP2

39438

w¹¹¹⁸; P{GMR67D12-GAL4}attP2

49405

w¹¹¹⁸; P{GMR60F05-GAL4}attP2

52823

w¹¹¹⁸; P{GMR60H04-lexA}attP40

54920

w¹¹¹⁸; P{GMR67C09-lexA}attP40

69594

w¹¹¹⁸; P{R60H04-p65.AD}attP40

69157

w¹¹¹⁸; P{R60F05-GAL4.DBD}attP2

69663

w¹¹¹⁸; P{R60H04-GAL4.DBD}attP2

VDRC

v42535

w¹¹¹⁸; P{GD10584}v42535

v41812

w¹¹¹⁸; P{GD10584}v41812/CyO

More stocks available...

Genomic Clones (34)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (137)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH11671

Other clones

DPiM_GH11671

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0004580

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0004580

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Reifegerste et al., 1993)

Commercially Available Antibodies

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

DCB32(pok13)

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Cbp53E has been cloned and molecularly characterised.

(Reifegerste et al., 1993, Reifegerste et al., 1991)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (17)

Reported As

Symbol Synonym

CALB2

(Wishart et al., 2012)

CALB2/calretinin

(Wishart et al., 2012)

CG6702

(National Institute of Genetics Fly Stocks, 2022-, Neuert et al., 2017, Çiçek et al., 2016, Hosono et al., 2015, White-Grindley et al., 2014, Keleman et al., 2009.8.5, Perkins et al., 2009)

Calb

(Fontenele et al., 2009)

Cbp33

(Fontenele et al., 2009)

Cbp53E

(Jetti et al., 2023, Gong et al., 2022, Velten et al., 2022, Kawamura et al., 2021, Al-Ramahi et al., 2018, Joos et al., 2018, Martinez et al., 2017, Neuert et al., 2017, Gene Disruption Project members, 2016-, Hagel et al., 2015, Bischof and FlyORF project members, 2014.6.20, White-Grindley et al., 2014, Japanese National Institute of Genetics, 2012.5.21, Mizrak et al., 2012, Jumbo-Lucioni et al., 2010, Fontenele et al., 2009)

DCBP

(Buchner, 1991)

Dcbp

Dcp

(Reifegerste et al., 1991)

cbn

(Reifegerste, 1993.6.11, Reifegerste, 1993.6.11, Reifegerste et al., 1993, Reifegerste, 1992.10.5)

cbp53E

(Prokop, 1999)

Name Synonyms

Calbindin

(Fontenele et al., 2009)

Calbindin 32

(Reifegerste et al., 1993)

Calbindin 53E

(Manh et al., 2005)

Calretinin

(Wishart et al., 2012)

calbindin

(Tessier and Broadie, 2011, Harteneck, 2003)

calbindin-32

(Reifegerste, 1993.6.11, Reifegerste, 1993.6.11)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 46 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36905

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P41044

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P41044

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A0B4LFM0

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P41044

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0004580

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36905/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004580

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004580

FlyMine - An integrated database for Drosophila genomics

FBgn0004580

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG6702

MARRVEL_MODEL - MARRVEL (model organism gene)

36905

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0004580

DroID - A comprehensive database of gene and protein interactions.

FBgn0004580

DRSC - Results frm RNAi screens

FBgn0004580

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

DCB32(pok13)

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004580

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0004580

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6702

Flygut - An atlas of the Drosophila adult midgut

FBgn0004580

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC008758

MIST (protein-protein) - An integrated Molecular Interaction Database

36905

References (70)

The following external sites may use different assemblies or annotations than FlyBase.

-0.71

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