FlyBase Gene Report: Dmel\fzy

FB2024_03 , released June 25, 2024

FB2024_03 , released June 25, 2024

Gene: Dmel\fzy

General Information

Symbol

Dmel\fzy

Species

D. melanogaster

Name

fizzy

Annotation Symbol

CG4274

Feature Type

protein_coding_gene

FlyBase ID

FBgn0001086

Gene Model Status

Stock Availability

21 publicly available

Gene Summary

fizzy (fzy) encodes WD40 domain protein required for the full ubiquitin ligase activity of the anaphase-promoting complex/cyclosome (APC/C) in mitosis and meiosis. The product of fzy functions to target substrates for destruction and drive metaphase and anaphase transition. [Date last reviewed: 2018-09-13] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Red Book
Interactive Fly

Also Known As

Cdc20, BG:DS02740.14

Key Links

Genomic Location

Cytogenetic map

Sequence location

2L:16,310,956..16,313,097 [+]

Recombination map

2-52

RefSeq locus

NT_033779 REGION:16310956..16313097

Sequence

Get Decorated FASTA

Genomic Maps

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (16 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables anaphase-promoting complex binding

inferred from electronic annotation with InterPro:IPR033010

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000460086

(GO Reference Genome Project, 2011-)

enables ubiquitin ligase activator activity

inferred from biological aspect of ancestor with PANTHER:PTN000460086

(GO Reference Genome Project, 2011-)

enables ubiquitin-protein transferase activator activity

inferred from electronic annotation with InterPro:IPR033010

(InterPro Project Members, 2004-)

Biological Process (9 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

involved_in anaphase-promoting complex-dependent catabolic process

inferred from mutant phenotype

(Swan and Schupbach, 2007)

inferred from direct assay

(Araki et al., 2005, Dawson et al., 1995)

involved_in female meiosis II

inferred from mutant phenotype

(Swan and Schupbach, 2007)

involved_in female meiosis I

inferred from genetic interaction with FLYBASE:cort; FB:FBgn0000351

(Swan and Schupbach, 2007)

involved_in germ-line stem cell population maintenance

inferred from mutant phenotype

(Chen et al., 2009)

involved_in mitotic cell cycle

inferred from high throughput mutant phenotype

(Ducat et al., 2008)

involved_in negative regulation of programmed necrotic cell death

inferred from mutant phenotype

(Kuang et al., 2014)

involved_in neuronal stem cell population maintenance

inferred from mutant phenotype

(Kuang et al., 2014)

involved_in protein catabolic process

inferred from mutant phenotype

(Raff et al., 2002)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in anaphase-promoting complex-dependent catabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000460086

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with HGNC:24824

(Gene Ontology Curators, 2002-)

involved_in positive regulation of anaphase-promoting complex-dependent catabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000460086

(GO Reference Genome Project, 2011-)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

part_of anaphase-promoting complex

inferred from direct assay

(Araki et al., 2005)

located_in centrosome

inferred from direct assay

(Raff et al., 2002)

located_in kinetochore

inferred from direct assay

(Raff et al., 2002)

located_in spindle

inferred from direct assay

(Raff et al., 2002)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of anaphase-promoting complex

inferred from biological aspect of ancestor with PANTHER:PTN000460086

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with HGNC:24824

(Gene Ontology Curators, 2002-)

Gene Group (FlyBase)

ANAPHASE-PROMOTING COMPLEX

Protein Family (UniProt)

-

Protein Signatures (InterPro)

Summaries

Gene Snapshot

fizzy (fzy) encodes WD40 domain protein required for the full ubiquitin ligase activity of the anaphase-promoting complex/cyclosome (APC/C) in mitosis and meiosis. The product of fzy functions to target substrates for destruction and drive metaphase and anaphase transition. [Date last reviewed: 2018-09-13]

Gene Group (FlyBase)

ANAPHASE-PROMOTING COMPLEX -: Anaphase-promoting complex/cyclosome (APC/C) is a multisubunit E3 ubiquitin ligase. Active during mitosis and G1 phases of the cell cycle, it mediates the degradation of cyclins and other key cell cycle regulators triggering the metaphase to anaphase transition and the exit from mitosis. (Adapted from PMID:12208841).

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

fzy: fizzy

Homozygous lethal; ventral outside and central nervous system degenerate.

Summary (Interactive Fly)

WD40 domain protein - homolog of Cdc20 - required for the activity of the anaphase promoting complex in mitosis - functions to target the three mitotic cyclins A, B and B3 for destruction in the egg and drive anaphase progression during meiosis

Interactive Fly

Gene Model and Products

Number of Transcripts

1

Number of Unique Polypeptides

1

Please see the JBrowse view of Dmel\fzy for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q24044)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.48

Sequence Ontology: Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0080833

1996

526

Additional Transcript Data and Comments

Reported size (kB)

1.9 (longest cDNA)

(Dawson et al., 1995)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

FBpp0080391

57.1

526

8.98

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

526 (aa); 59 (kD observed)

(Dawson et al., 1995)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\fzy using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

fzy_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.97

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 11 -- 12

central brain primordium

(Fisher et al., 2012)

embryonic central brain surface glial cell

(Fisher et al., 2012)

embryonic neuroblast of ventral nerve cord primordium

(Fisher et al., 2012)

lateral cord surface glial cell

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

procephalic neuroblast

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic central brain surface glial cell

(Fisher et al., 2012)

embryonic/larval nervous system

(Fisher et al., 2012)

lateral cord surface glial cell

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

embryonic stage 15

ventral nerve cord

(Magalhaes et al., 2007)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 5 -- 10

organism | ubiquitous

(Dawson et al., 1995)

embryonic stage 11

presumptive embryonic/larval central nervous system | presumptive

(Dawson et al., 1995)

embryonic stage 14

neuroblast | restricted

(Dawson et al., 1995)

ganglion mother cell | restricted

(Dawson et al., 1995)

Additional Descriptive Data

fzy protein is ubiquitous in stage 5 to 10 embryos, but its expression is restricted to proliferating cells after stage 11. The highest level of fzy protein in stage 11 embryos is found in the CNS precursors. At stage 14, the neuroblasts and ganglion mother cells at the edges of the ventral nerve cord and the brain lobes contain fzy protein.

(Dawson et al., 1995)

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of anaphase-promoting complex

inferred from direct assay

(Araki et al., 2005)

located_in centrosome

inferred from direct assay

(Raff et al., 2002)

located_in kinetochore

inferred from direct assay

(Raff et al., 2002)

located_in spindle

inferred from direct assay

(Raff et al., 2002)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\fzy in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\fzy

View exonic expression by tissue for Dmel\fzy

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001086

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34968/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001086

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001086

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001086

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4274

Flygut - An atlas of the Drosophila adult midgut

FBgn0001086

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\fzy

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 13 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 19 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of fzy

Transgenic constructs containing regulatory region of fzy

Aberrations (Deficiencies and Duplications) ( 64 )

Inferred from experimentation ( 64 )

Gene disrupted in

(Ryder, 2004.4.26)

(Ashburner et al., 1999, Dawson et al., 1995, Roth et al., 1995)

(Roote, 1996.8)

(Ashburner et al., 1999, Bergmann et al., 1996, Roote, 1996.8, Roth et al., 1995)

Df(2L)k08106-rv4

(Roote, 2000.6.13)

Df(2L)k08106-rv6

(Roote, 2000.6.13, Roote, 1999.7.7)

Df(2L)k08106-rv15

(Roote, 2000.6.13, Roote, 1999.7.7)

Df(2L)k14608-mr15

(Roote, 2000.6.13)

(Dawson et al., 1993, Ashburner et al., 1990)

(Eberl et al., 1998, Ashburner et al., 1990)

(Ashburner et al., 1990)

(Eberl et al., 1998, Dawson et al., 1995, Sigrist et al., 1995, Dawson et al., 1993, Ashburner et al., 1990)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

(Dawson et al., 1995, Ashburner et al., 1990)

(Ashburner et al., 1990)

Gene not disrupted in

(Ashburner et al., 1990)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

Df(2L)cact-255rv64

(Roote, 1996.1.11, Roote, 1996.8, Dawson et al., 1995)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

Df(2L)k17027^rv1

(Roote, 2000.6.13)

Df(2L)k17027^rv3

(Roote, 2000.6.13)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

(Eberl et al., 1998)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

Df(2L)TE35BC-34

(Ashburner et al., 1990)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

(Eberl et al., 1998, Dawson et al., 1995, Ashburner et al., 1990)

(Eberl et al., 1998, Ashburner et al., 1990)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

(Ashburner et al., 1990)

In(2L)dpp^s22Ldpp^d36R

(Ashburner et al., 1990)

(Eberl et al., 1998, Dawson et al., 1995, Ashburner et al., 1990)

In(2LR)Sco^rv1-DV7

(Ashburner et al., 1990)

(Ashburner et al., 1990)

Gene duplicated in

(Ashburner et al., 1990)

Gene not duplicated in

Dp(2;1)Sco^rv23

(Ashburner et al., 1990)

(Ashburner et al., 1990)

Dp(2;2)TE35B-54^L

(Ashburner et al., 1990)

In(2L)C163.41^LC158^R

(Ashburner et al., 1990)

In(2L)Sco^rv17LC158^R

(Ashburner et al., 1990)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^insc-Mz1407

fzy^GD11268

lethal, with Scer\GAL4^pnr-MD237

fzy^GD11268

lethal | recessive | heat sensitive

lethal - all die before end of pupal stage, with Scer\GAL4^ptc-559.1

fzy^UAS.cSa

lethal - all die before end of pupal stage | heat sensitive, with Scer\GAL4^en-e16E

fzy^UAS.cSa

lethal - all die during embryonic stage | recessive

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

fzy^GD11268

viable, with Scer\GAL4^tin.CΔ4

fzy^GD11268

Sterility

Allele

female sterile | recessive

Other Phenotypes

Allele

abnormal locomotor behavior | adult stage (with fzy¹)

fzy⁵⁰³²

abnormal locomotor behavior | adult stage (with fzy⁵⁰³²)

fzy¹

abnormal meiotic cell cycle | heat sensitive (with fzy⁶)

fzy⁷

abnormal meiotic cell cycle | heat sensitive (with fzy⁷)

fzy⁶

abnormal neuroanatomy | adult stage (with fzy¹)

fzy⁵⁰³²

abnormal neuroanatomy | adult stage (with fzy⁵⁰³²)

fzy¹

abnormal neuroanatomy | larval stage

fzy⁵⁰³²

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

fzy^GD11268

abnormal neuroanatomy | larval stage (with fzy¹)

fzy⁵⁰³²

abnormal neuroanatomy | larval stage (with fzy⁵⁰³²)

fzy¹

abnormal neuroanatomy | larval stage | progressive (with fzy¹)

fzy^AC-10

abnormal neuroanatomy | larval stage | progressive (with fzy^AC-10)

fzy¹

abnormal neuroanatomy | larval stage | somatic clone

abnormal size | adult stage, with Scer\GAL4^ey.PU

abnormal size | larval stage | somatic clone

fzy⁴

decreased cell number | larval stage (with fzy¹)

fzy⁵⁰³²

decreased cell number | larval stage (with fzy⁵⁰³²)

fzy¹

decreased occurrence of cell division | embryonic stage

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

fzy^GD11268

some die during embryonic stage | recessive

some die during pupal stage, with Scer\GAL4^ptc-559.1

fzy^UAS.cSa

some die during pupal stage | heat sensitive, with Scer\GAL4^en-e16E

fzy^UAS.cSa

visible, with Scer\GAL4^Bx-MS1096

fzy^HM04081

visible, with Scer\GAL4^en-e16E

fzy^UAS.cSa

visible, with Scer\GAL4^pnr-MD237

fzy^GD11268

fzy^tDa

Phenotype manifest in

Allele

adult head bristle, with Scer\GAL4^GMR.PU

embryonic/larval optic lobe | larval stage | somatic clone

fzy⁴

embryonic ventral epidermis

embryonic ventral nerve cord

fzy^unspecified

female germline stem cell | germline clone

fzy¹

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

fzy^GD11268

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

fzy^GD11268

mitosis & nuclear chromosome

fzy³

mitotic cell cycle

fzy³

mitotic cell cycle & epidermis & embryo | ventral

fzy¹

mitotic cell cycle & spindle & epidermis & embryo | ventral

fzy¹

neuroblast | larval stage

fzy⁵⁰³²

neuroblast | larval stage (with fzy¹)

neuroblast | larval stage (with fzy⁵⁰³²)

fzy¹

neuroblast | larval stage (with fzy^AC-10)

fzy¹

neuroblast | larval stage | somatic clone

neuropil | adult stage (with fzy¹)

fzy⁵⁰³²

neuropil | adult stage (with fzy⁵⁰³²)

fzy¹

retina, with Scer\GAL4^ey.PU

spindle | embryonic stage

trichogen cell, with Scer\GAL4^pnr-MD237

fzy^GD11268

wing, with Scer\GAL4^Bx-MS1096

fzy^HM04081

wing blade posterior compartment, with Scer\GAL4^en-e16E

fzy^UAS.cSa

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (14)

13 of 14

Yes

Yes

5 of 14

No

Yes

5 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1

1 of 14

No

No

1 of 14

No

No

1

1 of 14

No

No

11

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (9)

14 of 14

Yes

Yes

5 of 14

No

No

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Mus musculus (laboratory mouse) (10)

13 of 14

Yes

Yes

2

5 of 14

No

Yes

5 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Xenopus tropicalis (Western clawed frog) (10)

8 of 13

Yes

Yes

3 of 13

No

Yes

2 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

Danio rerio (Zebrafish) (10)

13 of 14

Yes

Yes

5 of 14

No

No

5 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Caenorhabditis elegans (Nematode, roundworm) (7)

7 of 14

Yes

Yes

3 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Anopheles gambiae (African malaria mosquito) (14)

Agam\AgaP_AGAP009338

12 of 12

Yes

Yes

Agam\AgaP_AGAP002114

5 of 12

No

No

Agam\AgaP_AGAP011622

2 of 12

No

No

Agam\AgaP_AGAP000479

1 of 12

No

No

Agam\AgaP_AGAP001362

1 of 12

No

No

Agam\AgaP_AGAP002019

1 of 12

No

No

Agam\AgaP_AGAP002221

1 of 12

No

No

Agam\AgaP_AGAP004827

1 of 12

No

No

Agam\AgaP_AGAP005359

1 of 12

No

No

Agam\AgaP_AGAP007125

1 of 12

No

No

Agam\AgaP_AGAP010318

1 of 12

No

No

Agam\AgaP_AGAP010685

1 of 12

No

No

Agam\AgaP_AGAP011621

1 of 12

No

No

Agam\AgaP_AGAP012731

1 of 12

No

No

Arabidopsis thaliana (thale-cress) (21)

12 of 13

Yes

Yes

12 of 13

Yes

Yes

12 of 13

Yes

Yes

12 of 13

Yes

Yes

12 of 13

Yes

Yes

10 of 13

No

Yes

5 of 13

No

No

5 of 13

No

No

5 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

Yes

Saccharomyces cerevisiae (Brewer's yeast) (9)

9 of 13

Yes

Yes

4 of 13

No

Yes

4 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

Schizosaccharomyces pombe (Fission yeast) (8)

11 of 12

Yes

Yes

4 of 12

No

No

3 of 12

No

Yes

Spom\SPAC13G6.08

3 of 12

No

Yes

3 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:fzy. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (11)

7 of 13

7 of 13

3 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Butzlaff et al., 2015)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CDC20; cell division cycle 20

13 of 14

OOCYTE/ZYGOTE/EMBRYO MATURATION ARREST 14; OZEMA14

CDC20B; cell division cycle 20B

5 of 14

FZR1; fizzy and cell division cycle 20 related 1

5 of 14

DEVELOPMENTAL AND EPILEPTIC ENCEPHALOPATHY 109; DEE109

developmental and epileptic encephalopathy 109

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

(Kuang et al., 2014)

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

On

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

anti bait coimmunoprecipitation, western blot, pull down, anti tag western blot

(Cunha-Silva et al., 2020, Derive et al., 2015, Conde et al., 2013, Li et al., 2010)

experimental knowledge based, pull down, anti tag western blot, anti tag coimmunoprecipitation

(Whitfield et al., 2013, Guruharsha et al., 2011, Chen et al., 2009)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Meghini et al., 2016)

anti bait coimmunoprecipitation, western blot

(Conde et al., 2013, Li et al., 2010)

anti tag coimmunoprecipitation, western blot, pull down, autoradiography

(Meghini et al., 2016)

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Kuang et al., 2014)

suppressible

(Kuang et al., 2014)

suppressible

(Jacobs et al., 2002)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Kuang et al., 2014)

suppressible

(Kuang et al., 2014)

suppressible

(Kuang et al., 2014)

enhanceable

(Leismann et al., 2000)

suppressible

(Friedman et al., 2011)

suppressible

(Kuang et al., 2014)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60979

DroID - A comprehensive database of gene and protein interactions.

FBgn0001086

MIST (genetic) - An integrated Molecular Interaction Database

34968

MIST (protein-protein) - An integrated Molecular Interaction Database

34968

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Ubiquitin mediated proteolysis

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q24044

Genomic Location and Detailed Mapping Data

Chromosome (arm)

2L

Recombination map

2-52

Cytogenetic map

Sequence location

2L:16,310,956..16,313,097 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

35F1-35F1

Limits computationally determined from genome sequence between P{lacW}heix^k11403&P{lacW}heix^k12401 and P{EP}fzy^EP1028&P{lacW}mRpL4^k14608

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

35F8-35F11

(determined by in situ hybridisation)

(Ashburner et al., 1999)

Experimentally Determined Recombination Data

Location

2-52

(FlyBase, 2016)

2-49.7

(Comeron et al., 2012)

2-51

(Tearle and Nusslein-Volhard, 1987)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (21)

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (133)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE39287

Other clones

FI02843

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0001086

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0001086

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Dawson et al., 1995)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Eggs derived from mutant females almost invariably arrest in anaphase of meiosis II.

(Swan and Schupbach, 2007)

cort and fzy are required for anaphase progression in both female meiotic divisions, performing partially redundant roles in meiosis I and non-redundant roles in meiosis II.

(Swan and Schupbach, 2007)

SL2 cells treated with dsRNA against fzy show a clear increase in the mitotic index compared to control cells.

(Muller et al., 2006)

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

(Stroschein-Stevenson et al., 2006)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R⁺ cells: cell morphology is aberrant and there is an increased frequency of microtubule-based mitotic spindles, indicative of a failure in mitosis.

(Kiger et al., 2003)

fzy is required for timely disengagement of mother and daughter centrioles in embryos.

(Vidwans et al., 1999)

The metaphase arrest seen in fzy mutants persists in the absence of pim or thr function.

(Philp and Glover, 1997)

fzy has been cloned and sequenced, and its expression pattern has been analysed.

(Dawson et al., 1995)

Mutations in fzy result in metaphase arrest, neither CycA, CycB or CycB3 are degraded in this arrest. Comparison of the fzy mutant phenotype with the phenotype resulting from expression of N-terminal truncated CycA, CycB or CycB3 suggests that fzy is not only required for mitotic cyclin degradation because fzy mutations, but not truncated cyclins, block chromosome separation. Inactivation of a temperature sensitive cdc2 in fzy metaphase arrest causes a reversion to interphase morphology. This reversion is not accompanied by completion of mitosis so there is no increase in anaphase cells or change in cell number or size.

(Sigrist et al., 1995)

The fizzy gene function is required maternally and zygotically for dividing cells to exit metaphase and complete mitosis. Zygotic fzy is required for normal differentiation of the ventral neurogenic ectoderm. Double mutant analysis shows that stg is epistatic to fzy.

(Dawson et al., 1993)

3 additional alleles are discussed but are not named.

(Ashburner et al., 1990)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: fzy CG4274

(Zhong, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (21)

Reported As

Symbol Synonym

BG:DS02740.14

(Ashburner et al., 1999, Ashburner et al., 1999.10.12)

CDC20

(Papoulas et al., 2010, Swan et al., 2005, Peters, 1999)

CG4274

(Bourquard et al., 2023, National Institute of Genetics Fly Stocks, 2022-, Çiçek et al., 2016, Butzlaff et al., 2015, Hosono et al., 2015, Schertel et al., 2013, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Pan and Zhang, 2009, Ducat et al., 2008, Ni et al., 2008)

Cdc20

(Cunha-Silva et al., 2020, Garrido et al., 2020, Moreno-Moreno et al., 2019, Martins et al., 2017, Derive et al., 2015, Kuang et al., 2014, Conde et al., 2013, Puram and Bonni, 2013, Li et al., 2010, Oliveira et al., 2010, Chia et al., 2008, Narbonne-Reveau et al., 2008, Somma et al., 2008, Swan and Schupbach, 2007, Tanaka-Matakatsu et al., 2007, Araki et al., 2005, Karess, 2005, Araki et al., 2003, Raff et al., 2002)

Cdc20/Fizzy

(van Roessel et al., 2004)

Cdc20/Fzy

(Swan and Schüpbach, 2007)

Cdc20^Fzy

(Grosskortenhaus and Sprenger, 2002)

Cdc20^fizzy

(Marshall, 2001)

Cdc20FZY

(Kohzaki et al., 2018)

FZY

(Joyce et al., 2012, Papoulas et al., 2010)

Fizzy

(Lehner, 2003)

Fzy

(Khalili et al., 2023, Bailey et al., 2021, Landmann et al., 2020, Martins et al., 2017, Duranteau et al., 2016, Meghini et al., 2016, Whitfield et al., 2013, Park and Asano, 2011, Buttitta et al., 2010, Khan et al., 2009, Narbonne-Reveau et al., 2008, Calzone et al., 2007, Li et al., 2007, Pandey et al., 2007, Acquaviva and Pines, 2006, Araki et al., 2005, Araki et al., 2003, Huang and Raff, 2002)

Fzy/Cdc20

(Garcia et al., 2007)

cdc2

(Singh et al., 2019)

cdc20

(Ly and Wang, 2020, Silva et al., 2018, Buttitta et al., 2010, Chen et al., 2009, Slack et al., 2007, Muller et al., 2006)

cdc20/fizzy

(Li and White, 2003)

dCDC20

(Chen et al., 2009)

dFZY

(Dobie et al., 1999)

fzy

(National Institute of Genetics Fly Stocks, 2022-, Li et al., 2021, Garrido et al., 2020, Blake-Hedges and Megraw, 2019, Hall et al., 2019, Hughes et al., 2018, Silva et al., 2018, Neuert et al., 2017, Transgenic RNAi Project members, 2017-, Das et al., 2016, Kong et al., 2016, Sarov et al., 2016, Butzlaff et al., 2015, Derive et al., 2015, Van Bortle et al., 2015, Kuang et al., 2014, Bonke et al., 2013, Schertel et al., 2013, Friedman et al., 2011, Neumüller et al., 2011, Zhang et al., 2011, Li et al., 2010, Chen et al., 2009, Dworkin et al., 2009, Scaria et al., 2008, Somma et al., 2008, Hueber et al., 2007, Magalhaes et al., 2007, Pesin and Orr-Weaver, 2007, Slack et al., 2007, Swan and Schupbach, 2007, Tanaka-Matakatsu et al., 2007, Ma et al., 2006, Stroschein-Stevenson et al., 2006, Liu et al., 2005, Loop et al., 2004, Savoian and Rieder, 2002)

fzy/cdc20

(Weber and Mlodzik, 2017)

Name Synonyms

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 83 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34968

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q24044

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q24044

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001086

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34968/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001086

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001086

FlyMine - An integrated database for Drosophila genomics

FBgn0001086

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG4274

MARRVEL_MODEL - MARRVEL (model organism gene)

34968

Linkouts

BioGRID - A database of protein and genetic interactions.

60979

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0001086

DroID - A comprehensive database of gene and protein interactions.

FBgn0001086

DRSC - Results frm RNAi screens

FBgn0001086

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

fzy_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001086

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0001086

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4274

Flygut - An atlas of the Drosophila adult midgut

FBgn0001086

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0001086

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC001383

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Ubiquitin mediated proteolysis

MIST (genetic) - An integrated Molecular Interaction Database

34968

MIST (protein-protein) - An integrated Molecular Interaction Database

34968

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q24044

References (200)