FB2024_03 , released June 25, 2024
Allele: Dmel\loafΔ20
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General Information
Symbol
Dmel\loafΔ20
Species
D. melanogaster
Name
FlyBase ID
FBal0368455
Feature type
allele
Associated gene
Dmel\loaf
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Douthit et al., 2021)
Progenitor genotype
Cytology
Description

A deletion creating a frameshift and a stop codon after 30 novel amino acids, removing the LDLa, transmembrane and cytoplasmic domains of the protein.

(Douthit et al., 2021)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3L:11,737,157..11,737,804 [-]
(Douthit et al., 2021)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      loafΔ20 clones show mis-targeting of R7 axons from the medulla M6 layer to the M3 layer.

      While loafΔ33/loafΔ20 mutants show no R7 axon targeting defects, the additional expression of loafUAS.Tag:HA under the control of Scer\GAL4GMR9D03 alone, Scer\GAL4dve-NP3428 and Scer\GAL4vg.PM combined or Scer\GAL4ap-md544 and Scer\GAL4NP1624 combined leads to mis-targeting of R7 axons from the medulla M6 layer to the M3 layer.

      (Douthit et al., 2021)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      loafΔ20
      (Douthit et al., 2021)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)