FlyBase Allele Report: Dmel\loaf[Δ33]

General Information

Symbol

Dmel\loaf^Δ33

Species

D. melanogaster

Name

FlyBase ID

FBal0368454

Feature type

allele

Associated gene

Dmel\loaf

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Douthit et al., 2021)

Progenitor genotype

Cytology

Description

An insertion-deletion creating a stop codon immediately following Thr 208 at the end of the CUB domain, removing the LDLa, transmembrane and cytoplasmic domains of the protein.

(Douthit et al., 2021)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

3L:11,737,157..11,737,799 [-]

Comment:

An insertion-deletion creating a stop codon immediately following Thr 208 at the end of the CUB domain. GATT is inserted at the site of the deletion.

Inserted_sequence:

GATT

(Douthit et al., 2021)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable

(Douthit et al., 2021)

viable (with loaf^Δ20)

(Douthit et al., 2021)

Phenotype Manifest In

axon terminus | adult stage, with Scer\GAL4^ap-md544, loaf^UAS.Tag:HA

(Douthit et al., 2021)

axon terminus | adult stage, with Scer\GAL4^ChAT.7.4, loaf^UAS.Tag:HA

(Douthit et al., 2021)

axon terminus | adult stage, with Scer\GAL4^VGlut-OK371, loaf^UAS.Tag:HA

(Douthit et al., 2021)

axon terminus | adult stage (with loaf^Δ20), with Scer\GAL4^dve-NP3428, Scer\GAL4^vg.PM, loaf^UAS.Tag:HA

(Douthit et al., 2021)

axon terminus | adult stage (with loaf^Δ20), with Scer\GAL4^GMR9D03, loaf^UAS.Tag:HA

(Douthit et al., 2021)

axon terminus | adult stage (with loaf^Δ20), with Scer\GAL4^NP1624, Scer\GAL4^ap-md544, loaf^UAS.Tag:HA

(Douthit et al., 2021)

axon terminus | adult stage | somatic clone

(Douthit et al., 2021)

outer medulla | adult stage, with Scer\GAL4^ap-md544, loaf^UAS.Tag:HA

(Douthit et al., 2021)

outer medulla | adult stage, with Scer\GAL4^ChAT.7.4, loaf^UAS.Tag:HA

(Douthit et al., 2021)

outer medulla | adult stage, with Scer\GAL4^VGlut-OK371, loaf^UAS.Tag:HA

(Douthit et al., 2021)

outer medulla | adult stage (with loaf^Δ20), with Scer\GAL4^dve-NP3428, Scer\GAL4^vg.PM, loaf^UAS.Tag:HA

(Douthit et al., 2021)

outer medulla | adult stage (with loaf^Δ20), with Scer\GAL4^GMR9D03, loaf^UAS.Tag:HA

(Douthit et al., 2021)

outer medulla | adult stage (with loaf^Δ20), with Scer\GAL4^NP1624, Scer\GAL4^ap-md544, loaf^UAS.Tag:HA

(Douthit et al., 2021)

outer medulla | adult stage | somatic clone

(Douthit et al., 2021)

photoreceptor cell R7 | adult stage, with Scer\GAL4^ap-md544, loaf^UAS.Tag:HA

(Douthit et al., 2021)

photoreceptor cell R7 | adult stage, with Scer\GAL4^ChAT.7.4, loaf^UAS.Tag:HA

(Douthit et al., 2021)

photoreceptor cell R7 | adult stage, with Scer\GAL4^VGlut-OK371, loaf^UAS.Tag:HA

(Douthit et al., 2021)

photoreceptor cell R7 | adult stage (with loaf^Δ20), with Scer\GAL4^dve-NP3428, Scer\GAL4^vg.PM, loaf^UAS.Tag:HA

(Douthit et al., 2021)

photoreceptor cell R7 | adult stage (with loaf^Δ20), with Scer\GAL4^GMR9D03, loaf^UAS.Tag:HA

(Douthit et al., 2021)

photoreceptor cell R7 | adult stage (with loaf^Δ20), with Scer\GAL4^NP1624, Scer\GAL4^ap-md544, loaf^UAS.Tag:HA

(Douthit et al., 2021)

photoreceptor cell R7 | adult stage | somatic clone

(Douthit et al., 2021)

Detailed Description

Statement

Reference

loaf^Δ33 clones show mis-targeting of R7 axons from the medulla M6 layer to the M3 layer.

While loaf^Δ33 mutants show no R7 axon targeting defects, the additional expression of loaf^UAS.Tag:HA under the control of Scer\GAL4^ap-md544, Scer\GAL4^ChAT.7.4 or Scer\GAL4^VGlut-OK371 leads to mis-targeting of R7 axons from the medulla M6 layer to the M3 layer.

While loaf^Δ33/loaf^Δ20 mutants show no R7 axon targeting defects, the additional expression of loaf^UAS.Tag:HA under the control of Scer\GAL4^GMR9D03 alone, Scer\GAL4^dve-NP3428 and Scer\GAL4^vg.PM combined or Scer\GAL4^ap-md544 and Scer\GAL4^NP1624 combined leads to mis-targeting of R7 axons from the medulla M6 layer to the M3 layer.