FB2024_03 , released June 25, 2024
Allele: Dmel\Ist151
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General Information
Symbol
Dmel\Ist151
Species
D. melanogaster
Name
FlyBase ID
FBal0353596
Feature type
allele
Associated gene
Dmel\Ist1
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Bäumers et al., 2019)
Mutagen
P-element activity
(Bäumers et al., 2019)
Progenitor genotype
P{EP}Ist1G6550
(Bäumers et al., 2019)
Cytology
Description

972bp of the Ist1 coding region, including the transcriptional start site, is deleted. Generated by imprecise excision of P{EP}Ist1G6550.

(Bäumers et al., 2019)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3L:6,755,483..6,756,454 [-]
Comment:

Curator inferred coordinates of a 972bp deletion resulting from the imprecise excision of P{EP}Ist1G6550 that extends from the insertion site into Ist1, removing the ATG and most of the coding region.

(Bäumers et al., 2019)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Vps609.2 wing disc clones do not show defects in cell polarity (assessed by DE-Cad and Dlg localization in immuno-stainings, and by Zonula adherens and septate junction integrity in TEM) or multivesicle body size.

      (Bäumers et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      Ist1[+]/Ist151, Vps609.2 has increased mortality during development phenotype, suppressible | partially by shrb[+]/shrb4

      (Bäumers et al., 2019)
      Enhancer of
      Statement
      Reference

      Ist1[+]/Ist151 is an enhancer of increased mortality during development phenotype of Vps609.2

      (Bäumers et al., 2019)

      Ist1[+]/Ist151 is an enhancer of increased mortality during development | cold sensitive phenotype of Vps609.2

      (Bäumers et al., 2019)
      Other
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Vps609.2, Ist151 double mutant wing disc clones raised at 29[o]C bulge out of the disc epithelium, fail to exhibit multivesicular bodies and instead exhibit enlarged endosomes and large vesicles that contain membraneous structures and electro-dense, but do not show cell polarity defects (assessed by DE-Cad and Dlg localization in immuno-stainings, and by Zonula adherens and septate junction integrity in TEM). When reared at 18[o]C, however, these double mutants are cell lethal in the wing disc, as no clones are obtained.

      (Bäumers et al., 2019)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Dist151
      (Bäumers et al., 2019)
      Ist151
      Name Synonyms
      Secondary FlyBase IDs
        References (1)