FB2024_03 , released June 25, 2024
Allele: Dmel\Vps609.2
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General Information
Symbol
Dmel\Vps609.2
Species
D. melanogaster
Name
FlyBase ID
FBal0353594
Feature type
allele
Associated gene
Dmel\Vps60
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Bäumers et al., 2019)
Mutagen
P-element activity
(Bäumers et al., 2019)
Progenitor genotype
P{EP}Vps60G4547
(Bäumers et al., 2019)
Cytology
Description

Deletion within Vps60 that removes the coding sequence downstream of exon 2. The predicted truncated protein lacks 154 of the 226 amino acid residues of wild-type Vps60. Generated by imprecise excision of P{EP}Vps60G4547. The deletion does not affect the adjacent downstream Eip74EF gene (determined by complementation analysis).

(Bäumers et al., 2019)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The rare Vps609.2 homozygous third instar larvae that survive when raised at 18[o]C develop melanotic tumors and their wing imaginal discs are very small, as compared to controls. In larvae reared at 25[o]C until the end of L2 and then shifted to 18[o]C until the end of L3, wing discs are larger than those kept constantly at 25[o]C and also larger than discs of wild type controls. Multivesicle bodies in wing disc cells are significantly larger than in wild-type controls, regardless of temperature, and contain more electron-dense material.

      Vps609.2 wing disc clones do not show cell polarity defects (assessed by DE-Cad and Dlg localization in immuno-stainings, and by Zonula adherens and septate junction integrity in TEM).

      (Bäumers et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Vps609.2, Ist151 double mutant wing disc clones raised at 29[o]C bulge out of the disc epithelium, fail to exhibit multivesicular bodies and instead exhibit enlarged endosomes and large vesicles that contain membraneous structures and electro-dense, but do not show cell polarity defects (assessed by DE-Cad and Dlg localization in immuno-stainings, and by Zonula adherens and septate junction integrity in TEM).When reared at 18[o]C, however, these double mutants are cell lethal in the wing disc, as no clones are obtained.

      (Bäumers et al., 2019)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Dchmp59.2
      (Bäumers et al., 2019)
      Vps609.2
      Name Synonyms
      Secondary FlyBase IDs
        References (1)