FB2024_03 , released June 25, 2024
Allele: Dmel\fussdelDS
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General Information
Symbol
Dmel\fussdelDS
Species
D. melanogaster
Name
FlyBase ID
FBal0349586
Feature type
allele
Associated gene
Dmel\fuss
Associated Insertion(s)
TI{TI}fussdelDS
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Rass et al., 2019)
Mutagen
CRISPR/Cas9
(Rass et al., 2019)
gene targeting by homologous recombination
(Rass et al., 2019)
Progenitor genotype
Associated Insertion(s)
TI{TI}fussdelDS
Cytology
Description

Deletion of an 855bp fragment which is shared by all three fuss transcripts (B, C and D). An attP site is introduced in the open reading frame of all fuss transcripts, which also results in a premature stop codon.

(Rass et al., 2019)
Allele components
Component
Use(s)
Also carries
attP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
4:964,774..965,628 [-]
Comment:

Approximate boundaries (based on CRISPR guide RNAs) of an 855bp deletion that affects all fuss transcripts. An attP site is introduced into the reading frame of fuss that results in a premature stop codon.

(Rass et al., 2019)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Bitter sensing is impaired in fussdelDS/fussdelDS, fussdelDS/fussMI13731 and fussdelDS.MI13731-GAL4/fussdelDS adults.

      fussMI13731/fussdelDS adults have significantly less Gr33a-positive and Gr66a-positive neurons than controls.

      fussdelDS/fussdelDS and fussdelDS/+ flies have a similar lifespan to controls.

      fussdelDS/fussdelDS adults have a normal mushroom body morphology.

      (Rass et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      fussdelDS/fussdelDS.MI13731-GAL4 is rescued by Scer\GAL4fuss-MI13731-GAL4/fussUAS.B

      (Rass et al., 2019)
      Comments

      Expressing fussUAS.B under the control of Scer\GAL4fuss-MI13731-GAL4 (the intrinsic Gal4 driver of fussdelDS.MI13731-GAL4) rescues the bitter sensing impairment of fussdelDS/fussdelDS.MI13731-GAL4 adults.

      (Rass et al., 2019)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      fussdelDS
      (Rass et al., 2020, Rass et al., 2019)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)